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Special Issue:
植物抗病遗传Plant Disease-resistance Genetics
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| Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm |
Sher MUHAMMAD1*, Muhammad SAJJAD2*, Sultan Habibullah KHAN3, Muhammad SHAHID1, Muhammad ZUBAIR1, Faisal Saeed AWAN3, Azeem Iqbal KHAN4, Muhammad Salman MUBARAK3, Ayesha TAHIR2, Muhammad UMER2, Rumana KEYANI2, Muhammad Inam AFZAL2, Irfan MANZOOR1, Javed Iqbal WATTOO5, Aziz-ur REHMAN6 |
1 Department of Bioinformatics and Biotechnology, GC University, Faisalabad 38090, Pakistan
2 Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad 45550, Pakistan
3 Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38000, Pakistan
4 Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan
5 Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54000, Pakistan
6 Wheat Research Institute, Ayub Agricultural Research Institute (AARI), Faisalabad 38950, Pakistan |
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Abstract Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance (APR) genes in newly developing wheat cultivars is the most judicious strategy to combat this disease. Herein, we dissected the genetics underpinning stripe rust resistance in Pakistani wheat germplasm. An association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic SSRs. Based on D´ measure, linkage disequilibrium (LD) exhibited between loci distant up to 45 cM. Marker-trait associations (MTAs) were determined using mixed linear model (MLM). Total 31 quantitative trait loci (QTLs) were observed on all 21 wheat chromosomes. Twelve QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat germplasm. The major QTLs were QYr.uaf.2AL and QYr.uaf.3BS (PVE, 11.9%). Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.
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Received: 08 August 2019
Accepted:
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| Fund: The authors acknowledge Higher Education Commission (HEC), Islamabad, Pakistan for providing for funding (21-179/SRGP/R&D/HEC/2014) and the International Research Support Initiative Program (IRSIP) Scholarship for this research work. |
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Corresponding Authors:
Correspondence Muhammad Sajjad, Tel: +92-334-6609080, E-mail: muhammad.sajjad@comsats.edu.pk; Sultan Habibullah KHAN, Tel: +92-333-9917733, E-mail: sultan@uaf.edu.pk
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| About author: * These authors contributed equally to this study. |
Cite this article:
Sher MUHAMMAD, Muhammad SAJJAD, Sultan Habibullah KHAN, Muhammad SHAHID, Muhammad ZUBAIR, Faisal Saeed AWAN, Azeem Iqbal KHAN, Muhammad Salman MUBARAK, Ayesha TAHIR, Muhammad Umer, Rumana KEYANI, Muhammad Inam AFZAL, Irfan MANZOOR, Javed Iqbal WATTOO, Aziz-ur REHMAN.
2020.
Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm. Journal of Integrative Agriculture, 19(8): 2035-2043.
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