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Special Issue:
油料作物合辑Oil Crops
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Exogenous strigolactones promote lateral root growth by reducing the endogenous auxin level in rapeseed |
MA Ni, WAN Lin, ZHAO Wei, LIU Hong-fang, LI Jun, ZHANG Chun-lei |
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs/Key Laboratory of Crop Physiology and Production, Ministry of Agriculture and Rural Affairs, Wuhan 430062, P.R.China |
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Abstract Strigolactones (SLs) are newly discovered plant hormones which regulate the normal development of different plant organs, especially root architecture. Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield. Here, we treated the seedlings of Zhongshuang 11 (ZS11), an elite conventional rapeseed cultivar, with different concentrations of GR24 (a synthetic analogue of strigolactones), and found that a low concentration (0.18 µmol L–1) of GR24 could significantly increase the lateral root growth, shoot growth, and root/shoot ratio of seedlings. RNA-Seq analysis of lateral roots at 12 h, 1 d, 4 d, and 7 d after GR24 treatment showed that 2 301, 4 626, 1 595, and 783 genes were significantly differentially expressed, respectively. Function enrichment analysis revealed that the plant hormone transduction pathway, tryptophan metabolism, and the phenylpropanoid biosynthesis pathway were over-represented. Moreover, transcription factors, including AP2/ERF, AUX/IAA, NAC, MYB, and WRKY, were up-regulated at 1 d after GR24 treatment. Metabolomics profiling further demonstrated that the amounts of various metabolites, such as indole-3-acetic acid (IAA) and cis-zeatin were drastically altered. In particular, the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8% at 12 h and 1 d after GR24 treatment, respectively. Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones, which provides useful clues for the effects of SLs on root architecture and crop productivity.
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Received: 08 October 2018
Accepted:
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| Fund: Funds were provided by the National Key Research and Development Program of China (2018YFD1000900). |
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Corresponding Authors:
Correspondence MA Ni, E-mail: mani@caas.cn; ZHANG Chun-lei, Tel: +86-27-86739796, Fax: +86-27-86816451, E-mail: zhangchunlei@caas.cn
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Cite this article:
MA Ni, WAN Lin, ZHAO Wei, LIU Hong-fang, LI Jun, ZHANG Chun-lei.
2020.
Exogenous strigolactones promote lateral root growth by reducing the endogenous auxin level in rapeseed . Journal of Integrative Agriculture, 19(2): 465-482.
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