maize (Zea mays L.) , kernel , RNA-seq , mitosis-storage accumulation , transition , endosperm," /> maize (Zea mays L.) , kernel , RNA-seq , mitosis-storage accumulation , transition , endosperm,"/> maize (Zea mays L.) , kernel , RNA-seq , mitosis-storage accumulation , transition , endosperm,"/>
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Journal of Integrative Agriculture  2018, Vol. 17 Issue (07): 1574-1584    DOI: 10.1016/S2095-3119(18)61956-2
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq
ZHOU Yu-qian1*, WANG Qin-yang2*, ZHAO Hai-liang2, GONG Dian-ming2, SUN Chuan-long2, REN Xue-mei2, LIU Zhong-xiang1, HE Hai-jun1, QIU Fa-zhan
1 Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China
2 National Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China
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Seed size play a significant role in maize yield production.  Two maize inbred lines with distinct seed size and weight, V671 (a large-seed inbred line) and Mc (a small-seed inbred line), were investigated by RNA-seq at 14 days after pollination (DAP), when maize endosperm undergoes an active transition from mitosis to storage accumulation.  RNA-seq expression data showed that the small-seed line Mc had a higher storage accumulation activity, whereas the large-seed kernel line V671 possessed a higher DNA synthesis activity.  An investigation of the pattern of increasing kernel width at serial DAPs showed that V671 experienced an increased kernel width later than did Mc, but the rate and duration of increase were longer in V671.  SDS-PAGE of the storage proteins and quantitative RT-PCR of cell cycle-related genes and indole-3-acetic (IAA) synthesis genes certified that the transition from mitosis to storage accumulation starts earlier in Mc.  We hypothesized that the difference in the mitosis-to-storage accumulation transition accounts for the larger seed size in V671 vs. Mc.
Keywords:  maize (Zea mays L.) ')" href="#">  
Received: 21 December 2017   Accepted: 05 July 2018
Fund: This research was supported by the National Natural Science Foundation of China (91735306), the National Basic Research Program of China (973 Program, 2014CB138203), and the National Key Research and Development Program of China (2016YFD0100103-19).
Corresponding Authors:  Correspondence QIU Fa-zhan, Tel: +86-27-87286870, Fax: +86-27-87281006, E-mail:    
About author:  * These authors contributed equally to this study.
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ZHOU Yu-qian
WANG Qin-yang
ZHAO Hai-liang
GONG Dian-ming
SUN Chuan-long
REN Xue-mei
LIU Zhong-xiang
HE Hai-jun
QIU Fa-zhan

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ZHOU Yu-qian, WANG Qin-yang, ZHAO Hai-liang, GONG Dian-ming, SUN Chuan-long, REN Xue-mei, LIU Zhong-xiang, HE Hai-jun, QIU Fa-zhan. 2018. Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq. Journal of Integrative Agriculture, 17(07): 1574-1584.

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