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Special Issue:
玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources
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| Comparative transcriptome analysis of different nitrogen responses in low-nitrogen sensitive and tolerant maize genotypes |
DU Qing-guo1, 2*, YANG Juan1*, Shah SYED MUHAMMAD SADIQ1, YANG Rong-xin1, YU Jing-juan2, LI Wen-xue1 |
1 National Engineering Laboratory for Crop Molecular Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China |
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摘要
尽管目前的研究极大地促进了我们对于植物适应低氮胁迫的认知，但是关于不同作物基因型适应低氮胁迫能力不同的机制仍需要进一步探讨。本文中，我们根据田间条件下304份玉米自交系对低氮胁迫的耐受性，从中选择了Ye478（低氮胁迫敏感材料）和Qi319（耐受低氮胁迫材料）进行进一步的研究。首先我们对Ye478和Qi319正常氮水培和低氮水培的地上部和根部构建了16个转录组文库，并进行高通量测序。结果分析发现Qi319根系中特异上调表达的基因主要富集在代谢能相关途径，包括三羧酸代谢过程和烟酰胺代谢过程。在低氮胁迫处理5天后，仅在Ye478中观察到老叶变黄的表型；与Qi319相比，在Ye478地上部特异下调表达的基因主要与类囊体、叶绿体、光合膜和叶绿体基质等有关。对转录因子进行分析，共有216个转录因子在Ye478和Qi319之间差异表达，表明氮胁迫响应路径中的转录调控在不同作物基因型适应低氮胁迫中起重要作用。此外，在Ye478和Qi319中还发现了15个差异表达的miRNAs。综上所述，我们的研究有助于了解玉米耐受低氮胁迫的遗传变异和分子基础。
Abstract Although previous researches have greatly increased our general knowledge on plant responses to nitrogen (N) stress, a comprehensive understanding of the different responses in crop genotypes is still needed. This study evaluated 304 maize accessions for low-N tolerance under field conditions, and selected the low-N sensitive Ye478 and low-N tolerant Qi319 for further investigations. After a 5-day low-N treatment, the typical N-deficient phenotype with yellowing older leaves was observed in Ye478 but not in Qi319. After the 5-day low-N stress, 16 RNA libraries from leaf and root of Ye478 and Qi319 were generated. The differentially expressed genes (DEGs) in the root of Qi319 up-regulated by special N deficiency were mainly enriched in energy-related metabolic pathways, including tricarboxylic acid metabolic process and nicotinamide metabolic process. Consistent with yellowing older leaves only observed in Ye478, the special N deficiency-responsive DEGs related to thylakoid, chloroplast, photosynthetic membrane, and chloroplast stroma pathways were repressed by low-N stress in Ye478. A total of 216 transcription factors (TFs), including ZmNLP5, were identified as special N deficiency-responsive TFs between Qi319 and Ye478, indicating the importance of transcriptional regulation of N stress-responsive pathway in different tolerance to low-N stress between crop genotypes. In addition, 15 miRNAs were identified as DEGs between Qi319 and Ye478. Taken together, this study contributes to the understanding of the genetic variations and molecular basis of low-N tolerance in maize.
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Received: 05 February 2020
Accepted:
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| Fund: This work was supported by grants from the Ministry of Agriculture of China for Transgenic Research (2018ZX0800916B), the National Natural Science Foundation of China (31861143004) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences. |
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Corresponding Authors:
Correspondence LI Wen-xue, Tel: +86-10-82105799, E-mail: liwenxue@caas.cn
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| About author: DU Qing-guo, E-mail: duqingguo2011@163.com; YANG Juan, E-mail: 13051377865@126.com; * These authors contributed equally to this study. |
Cite this article:
DU Qing-guo, YANG Juan, Shah SYED MUHAMMAD SADIQ, YANG Rong-xin, YU Jing-juan, LI Wen-xue.
2021.
Comparative transcriptome analysis of different nitrogen responses in low-nitrogen sensitive and tolerant maize genotypes. Journal of Integrative Agriculture, 20(8): 2043-2055.
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