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Special Issue:
农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
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| Modification of total and phosphorus mineralizing bacterial communities associated with Zea mays L. through plant development and fertilization regimes |
XIN Yuan-yuan1*, Anisur RAHMAN1*, LI Hui-xiu1, XU Ting1, 2, DING Guo-chun1, 2, LI Ji1, 2 |
1 College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R.China
2 Organic Recycling Institute (Suzhou), China Agricultural University, Suzhou 215128, P.R.China |
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摘要
根际微生物群及其中的解磷细菌在维持集约化农业系统的可持续性和生产力方面具有重要潜力。施肥制度能够影响土壤微生物群落，然而目前仍不清楚土壤中哪些群落的变化会影响有益菌群在根际的富集。本研究基于曲周实验站的一个长期定位实验，该实验始于1993年，含有堆肥、生物堆肥、化肥或不施肥的四个不同处理。利用选择性培养法、16S rRNA基因的Illumina测序，比较了不同处理下玉米根际总细菌和解磷细菌的多样性和群落结构的动态。研究结果显示：玉米的发育期是影响根际细菌和解磷细菌种群结构的主要因子，分别能够解释29%和13%的变异，作用高于不同的施肥。施用堆肥或生物堆肥的土壤中副球菌属的相对丰度显著高于施用化肥或不施肥处理。在根际富集的属中，海洋芽胞杆菌属、芽孢杆菌属、无色杆菌属、剑菌属、 副球菌属、Ramlibacter和藤黄单胞菌属的相对丰度与其在土壤中的相对丰度呈正相关。解磷细菌中剑菌属、芽孢杆菌属和链霉菌属的相对丰度也表现出上述规律。综上所述，尽管玉米发育期是影响根际微生物群的主要因素，但施肥制度或也能够影响根际有益微生物群，如芽孢杆菌属和剑菌属。
Abstract Harnessing the rhizospheric microbiome, including phosphorus mineralizing bacteria (PMB), is a promising technique for maintaining sustainability and productivity in intensive agricultural systems. However, it is unclear as to which beneficial taxonomic group populations in the rhizosphere are potentially associated with the changes in soil microbiomes shifted by fertilization regimes. Herein, we analyzed the diversity and community structure of total bacteria and PMB in the rhizosphere of maize (Zea mays L.) grown in soils under 25 years of four fertilization regimes (compost, biocompost, chemical, or non-fertilized) via selective culture and Illumina sequencing of the 16S rRNA genes. Plant development explained more variations (29 and 13%, respectively) in the composition of total bacteria and PMB in the rhizosphere of maize than the different fertilization regimes. Among those genera enriched in the rhizosphere of maize, the relative abundances of Oceanobacillus, Bacillus, Achromobacter, Ensifer, Paracoccus, Ramlibacter, and Luteimonas were positively correlated with those in the bulk soil. The relative abundance of Paracoccus was significantly higher in soils fertilized by compost or biocompost than the other soils. Similar results were also observed for PMB affiliated with Ensifer, Bacillus, and Streptomyces. Although plant development was the major factor in shaping the rhizospheric microbiome of maize, fertilization regimes might have modified beneficial rhizospheric microbial taxa such as Bacillus and Ensifer.
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Received: 09 June 2020
Accepted:
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| Fund: This work was supported by the National Key R&D Program of China (2019YFD1002000, 2016YFD0800602 and 2016YFD0501404). |
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Corresponding Authors:
Correspondence DING Guo-chun, Tel: +86-10-62732017, E-mail: gc_ding@cau.edu.cn
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| About author: XIN Yuan-yuan, E-mail: 1213446227@qq.com; Anisur RAHMAN, E-mail: anisdu05@yahoo.com; * These authors contributed equally to this study. |
Cite this article:
XIN Yuan-yuan, Anisur RAHMAN, LI Hui-xiu, XU Ting, DING Guo-chun, LI Ji.
2021.
Modification of total and phosphorus mineralizing bacterial communities associated with Zea mays L. through plant development and fertilization regimes. Journal of Integrative Agriculture, 20(11): 3026-3038.
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