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Journal of Integrative Agriculture  2020, Vol. 19 Issue (9): 2265-2273    DOI: 10.1016/S2095-3119(20)63186-0
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Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes
YANG Xiao-xiang1, 2, HUANG Xiao-qin1, 2, WU Wen-xian1, XIANG Yun-jia1, DU Lei1, ZHANG Lei1, 2, LIU Yong1, 2 
1 Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
2 Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 610066, P.R.China
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Clubroot disease, caused by Plasmodiophora brassicae, is one of the most destructive soil-borne diseases in cruciferous crops worldwide.  New strategies are urgently needed to control this disease, as no effective disease-resistant varieties or chemical control agents exist.  Previously, we found that the incidence rate and disease index of clubroot in oilseed rape decreased by 50 and 40%, respectively, when oilseed rape was planted after soybean.  In order to understand how different rotation patterns affect the occurrence of clubroot in oilseed rape, high-throughput sequencing was used to analyze the rhizosphere microbial community of oilseed rape planted after leguminous (soybean, clover), gramineous (rice, maize) and cruciferous (oilseed rape, Chinese cabbage) crops.  Results showed that planting soybeans before oilseed rape significantly increased the population density of microbes that could inhibit P. brassicae (e.g., Sphingomonas, Bacillus, Streptomyces and Trichoderma).  Conversely, consecutive cultivation of cruciferous crops significantly accumulated plant pathogens, including P. brassicae, Olpidium and Colletotrichum (P<0.05).  These results will help to develop the most effective rotation pattern for reducing clubroot damage.
Keywords:  oilseed rape        clubroot        Gramineae        Leguminosae        Cruciferae        rhizosphere soil  
Received: 15 October 2019   Accepted: 04 August 2020
Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0200600), the Financial Innovation Capacity Enhancement Project in Sichuan Province, China (2019QNJJ-011), and the National Modern Agricultural Industry Technology System of Sichuan Rape Innovation Team, China (2019–2023).
Corresponding Authors:  Correspondence ZHANG Lei, Tel/Fax: +86-28-84504089, E-mail:; LIU Yong, Tel/Fax: +86-28-84504089, E-mail:   
About author:  YANG Xiao-xiang, E-mail:;

Cite this article: 

YANG Xiao-xiang, HUANG Xiao-qin, WU Wen-xian, XIANG Yun-jia, DU Lei, ZHANG Lei, LIU Yong. 2020. Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes. Journal of Integrative Agriculture, 19(9): 2265-2273.

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