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Journal of Integrative Agriculture  2017, Vol. 16 Issue (10): 2239-2245    DOI: 10.1016/S2095-3119(16)61594-0
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Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea
CHEN Guo-hua1, TIAN Xue-liang2, WANG Dian-dong3, LING Jian1, MAO Zhen-chuan1, YANG Yu-hong1, XIE Bing-yan1
1 Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture, Beijing 100081, P.R.China
2 College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, P.R.China
3 Life Science and Technology Institute, Yangtze Normal University, Fuling 408100, P.R.China
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Abstract  The expression of mitogen-activated protein kinase (mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita.  However, little is known about the ecological effects of transgenic plants.  Here, we analyzed the diversity of 16S rDNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change.  A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk dsRNA-expressing plants, respectively.  No significant difference was observed between the two groups according to Shannon and Simpson indices.  In soil samples of the two rhizospheres, the dominant group was Crenarchaeota at the phylum level, with Staphylothermus, Methanococcus, Pyrodictium and Sulfolobus the abundant taxa at the genus level.  These results suggest that expressing mapk double-stranded (ds) RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea, and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk dsRNA.   
Keywords:  cucumber        16S rDNA        diversity        rhizosphere archaea        mapk dsRNA  
Received: 19 December 2016   Accepted:

This research was supported by the National Natural Science Foundation of China (30900926), funded by the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the China Agriculture Research System (CARS-23-D01).

Corresponding Authors:  Correspondence XIE Bing-yan, Tel: +86-10-82109546, Fax: +86-10-62174123, E-mail:   
About author:  CHEN Guo-hua, E-mail:;

Cite this article: 

CHEN Guo-hua, TIAN Xue-liang, WANG Dian-dong, LING Jian, MAO Zhen-chuan, YANG Yu-hong, XIE Bing-yan. 2017. Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea. Journal of Integrative Agriculture, 16(10): 2239-2245.

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