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Journal of Integrative Agriculture  2021, Vol. 20 Issue (10): 2706-2715    DOI: 10.1016/S2095-3119(21)63609-2
Special Issue: 植物病理合辑Plant Protection—Plant Pathology 线虫合辑Nematology
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Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria
Fisayo Y. DARAMOLA1, Osarenkhoe O. OSEMWEGIE1, Stephen O. OWA1, Samuel B. ORISAJO2, Evbuomwan IKPONMWOSA1, Elizabeth T. ALORI3 
1 Department of Microbiology, Landmark University, Omu Aran 370102, Kwara State, Nigeria
2 Crop Protection Division, Cocoa Research Institute of Nigeria, Ibadan 200257, Oyo State, Nigeria
3 Department of Crop and Soil Sciences, Landmark University, Omu Aran 370102, Kwara State, Nigeria
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The occurrence of entomopathogenic nematodes (EPNs) in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait (White’s trap) techniques.  Isolates were tested for infectivity using the larvae of Galleria mellonella (greater moth) and Tenebrio molitor (mealworm).  The study revealed a new species of Heterorhabditis (MT371593) in soil samples that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University, Nigeria.   Amplification of the internal transcribed spacer region (ITS) of the ribosomal DNA produced a nucleotide sequence of 933 base pairs (bp).  A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99% similarity to that of Heterorhabditis sp. from Thailand.   Infectivity test of the isolate showed 100% mortality against T. molitor larvae within 48 h of exposure while only 80% mortality was recorded for G. mellonella after 1 week of exposure.  This is the first account of Heterorhabditis sp. in Nigeria.  The varying degrees of infectivity against mealworm and greater moth observed in this study proved that the Nigerian isolate of Heterorhabditis sp. could potentially be an attractive option in the management of insect pests of cash crops.
Keywords:  biological control        EPNs        Heterorhabditis sp.        ribosomal DNA        Tenebrio molitor  
Received: 27 May 2020   Accepted:
Fund: The authors are grateful to the Management of Landmark University for financial assistance and to Prof. A. P. Malan of the Department of Conservation Ecology & Entomology, Stellenbosch University, South Africa for providing guidance with molecular identification.
Corresponding Authors:  Correspondence Fisayo Y. DARAMOLA, E-mail:,   

Cite this article: 

Fisayo Y. DARAMOLA, Osarenkhoe O. OSEMWEGIE, Stephen O. OWA, Samuel B. ORISAJO, Evbuomwan IKPONMWOSA, Elizabeth T. ALORI. 2021. Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria. Journal of Integrative Agriculture, 20(10): 2706-2715.

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