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| The key residues of OsTFIIAγ5/Xa5 protein captured by the arginine-rich TFB domain of TALEs compromising rice susceptibility and bacterial pathogenicity |
| TIAN Jing-jing, HUI Shu-gang, SHI Ya-rui, YUAN Meng |
| National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, P.R.China |
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Abstract Xanthomonas bacteria secrete transcription activator-like effector (TALE) proteins into host cells to activate plant disease susceptibility genes to cause disease, and the process is dependent on interaction between bacteria TFB domain of TALEs and host plant basal transcription factor IIA gamma subunit (TFIIAγ). The key domain or residues of plant TFIIAγ and core residues of bacteria TFB domain that are indispensable for TFIIAγ-TALEs interaction in the process of TALE-carrying Xanthomonas invasion plants are unknown. Here, we showed that the third α-helix domain of OsTFIIAγ5/Xa5, especially the 38th, 39th, 40th and 42th residues were key sites for capturing by TALEs of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease. The latter segment of Xoo TFB domain harboring seventy-two amino acid residues was vital for TALE specific binding with host plant OsTFIIAγ5/Xa5. Substitution of some residues in this core region of TFB domain completely compromised capacity of TALEs capturing rice OsTFIIAγ5/Xa5. The rich and conserved arginine residues in this core region of TFB domain were responsible for TALE-dependent plant susceptibility gene activation and virulence of Xoo. These results provide a potential strategy for improving resistance to TALE-carrying pathogens in plants by site-specific modification of key residues of host plant TFIIAγ.
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Received: 09 May 2018
Accepted:
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| Fund: This work was supported by the grants from the National Natural Science Foundation of China (31822042 and 31871946) and the Fundamental Research Funds for the Central Universities, China (2662016PY020 and 2662017PY014). |
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Corresponding Authors:
Correspondence YUAN Meng, Tel: +86-
27-87281812, E-mail: myuan@mail.hzau.edu.cn
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| About author: TIAN Jing-jing, Tel: +86-27-87281812, E-mail: 2017304110109@
webmail.hzau.edu.cn; |
Cite this article:
TIAN Jing-jing, HUI Shu-gang, SHI Ya-rui, YUAN Meng.
2019.
The key residues of OsTFIIAγ5/Xa5 protein captured by the arginine-rich TFB domain of TALEs compromising rice susceptibility and bacterial pathogenicity. Journal of Integrative Agriculture, 18(6): 1178-1188.
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