|
|
|
|
|
| Structure and expression analysis of the sucrose synthase gene family in apple |
| TONG Xiao-lei1*, WANG Zheng-yang1*, MA Bai-quan1, ZHANG Chun-xia2, ZHU Ling-cheng1, MA Feng-wang1, LI Ming-jun1 |
1 State Key Laboratory of Crop Stress Biology for Arid Areas (SKLCSBAA)/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 College of Forestry, Northwest A&F University, Yangling 712100, P.R.China |
|
|
|
|
Abstract Sucrose synthases (SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica (MdSUSs), and phylogenetic analysis revealed that the MdSUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, MdSUS1s and MdSUS2.1 showed the highest expression in fruit, whereas MdSUS2.2/2.3 and MdSUS3s exhibit the highest expression in shoot tips. Most MdSUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both MdSUS2.1 and MdSUS1.4 displayed opposite expression profiles. These results suggest that different MdSUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.
|
|
Received: 13 March 2017
Accepted:
|
| Fund: This work was supported in part by the National Natural Science Foundation of China (31372038) and the Natural Basic Research Plan in Shaanxi Province of China (2015JQ3082). |
|
Corresponding Authors:
Correspondence LI Ming-jun, Tel/Fax: +86-29-87082613, E-mail: limingjun@nwafu.edu.cn
|
| About author: TONG Xiao-lei, E-mail: xiaoleitong322@163.com; WANG Zheng-yang, E-mail: 691319339@qq.com;
* These authors contributed equally to this study. |
Cite this article:
TONG Xiao-lei, WANG Zheng-yang, MA Bai-quan, ZHANG Chun-xia, ZHU Ling-cheng, MA Feng-wang, LI Ming-jun.
2018.
Structure and expression analysis of the sucrose synthase gene family in apple. Journal of Integrative Agriculture, 17(04): 847-856.
|
| Albrecht G, Mustroph A. 2003. Localization of sucrose synthase in wheat roots: Increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia. Planta, 217, 252–260.An X, Chen Z, Wang J, Ye M, Ji L, Wang J, Liao W, Ma H. 2014. Identification and characterization of the Populus sucrose synthase gene family. Gene, 539, 58–67.Baud S, Vaultier M N, Rochat C. 2004. Structure and expression profile of the sucrose synthase multi-gene family in Arabidopsis. Journal of Experimental Botany, 55, 397–409. Chang S, Puryear J, Cairney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter, 11, 113–116.Chen A Q, He S, Li F F, Li Z, Ding M Q, Liu Q P, Rong J K. 2012. Analyses of the sucrose synthase gene family in cotton: Structure, phylogeny and expression patterns. BMC Plant Biology, 12, 85.Fujii S, Hayashi T, Mizuno K. 2010. Sucrose synthase is an integral component of the cellulose synthesis machinery. Plant Cell Physiology, 51, 294–301.Gaudin V, Lunness P A, Fobert P R, Towers M, Riou-Khamlichi C, Murray J A, Coen E, Doonan J H. 2000. The expression of Dcyclin genes defines distinct developmental zones in snapdragon apical meristems and is locally regulated by the Cycloidea gene. Plant Physiology, 122, 1137–1148.Geigenberger P, Stitt M. 1993. Sucrose synthase catalyzes a readily reversible reaction in vivo in developing potato tubers and other plant tissues. Planta, 189, 329–339.Harada T, Satoh S, Yoshioka T, Ishizawa K. 2005. Expression of sucrose synthase genes involved in enhanced elongation of pondweed (Potamogeton distinctus) turions under anoxia. Annals of Botany, 96, 683–692.Hirose T, Scofield G N, Terao T. 2008. An expression analysis profile for the entire sucrose synthase gene family in rice. Plant Science, 174, 534–543.Horst I, Welham T, Kelly S, Kaneko T, Sato S, Tabata S, Parniske M, Wang T L. 2007. Tilling mutants of Lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of nodule-enhanced sucrose synthase. Plant Physiology, 144, 806–820.Hu L F, Liu S Q. 2011. Genome-wide identification and phylogenetic analysis of the ERF gene family in cucumbers. Genetics and Molecular Biology, 34, 624–633.Iraqi D, Tremblay F M. 2001. Analysis of carbohydrate metabolism enzymes and cellular contents of sugars and proteins during spruce somatic embryogenesis suggests a regulatory role of exogenous sucrose in embryo development. Journal of Experimental Botany, 52, 2301–2311.Kleczkowski L A, Kunz S, Wilczynska M. 2010. Mechanisms of UDP-Glucose synthesis in plants. Critical Reviews in Plant Sciences, 29, 191–203.Koch K E. 2004. Sucrose metabolism: Regulatory mechanisms and pivotal roles in sugar sensing and plant development. Current Opinion in Plant Biology, 7, 235–246.Komatsu A, Moriguchi T, Koyama K, Omura M, Akihama T. 2002. Analysis of sucrose synthase genes in citrus suggests different roles and phylogenetic relationships. Journal of Experimental Botany, 53, 61–71.Li M, Feng F, Cheng L. 2012. Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development. PLoS ONE, 7, e33055.Lutfiyya L L, Xu N F, D’Ordine R L, Morrell J A, Miller P W, Duff S M G. 2007. Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants. Journal of Plant Physiology, 164, 923–933.Ohto M, Onai K, Furukawa Y, Aoki E, Araki T, Nakamura K. 2001. Effects of sugar on vegetative development and floral transition in Arabidopsis. Plant Physiology, 127, 252–261.Rook F, Corke F, Card R, Munz G, Smith C, Bevan M W. 2001. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling. The Plant Journal, 26, 421–433.Ruan Y L, Llewellyn D J, Liu Q, Xu S M, Wu L M, Wang L, Furbank R T. 2008. Expression of sucrose synthase in the developing endosperm is essential for early seed development in cotton. Functional Plant Biology, 35, 382–393.Sturm A, Tang G Q. 1999. The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends in Plant Science, 4, 401–407.Velasco R, Zharkikh A, Affourtit J, Dhingra A, Bhatnagar S K. 2010. The genome of the domesticated apple (Malus×domestica Borkh). Nature Genetics, 42, 833–839. Wei X, Liu F, Chen C, Ma F, Li M. 2014. The Malus domestica sugar transporter gene family: Identifications based on genome and expression profiling related to the accumulation of fruit sugars. Frontiers in Plant Science, 5, 569.Xiao X, Tang C, Fang Y, Yang M, Zhou B, Qi J, Zhang Y. 2014. Structure and expression profile of the sucrose synthase gene family in the rubber tree: Indicative of roles in stress response and sucrose utilization in the laticifers. The FEBS Journal, 281, 291–305.Yagi T, Baroja-Fernandez E, Yamamoto R. 2003. Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose. Biochemical Journal, 370, 409–415.Yamaki S, Ino M. 1992. Alteration of cellular compartmentation and membrane-permeability to sugars in immature and mature apple fruit. Journal of the American Society for Horticultural Science, 117, 951–954.Yamaki S, Ishikawa K. 1986. Roles of four sorbitol related enzymes and invertase in the seasonal. Alleration of sugar metabolism in apple tissue. Journal of the American Society for Horticultural Science, 111, 134–137.Zhang C H, Yu M L Ma R J, Shen Z J, Zhang B B, Nicholas K K. 2015. Structure, expression profile, and evolution of the sucrose synthase gene family in peach (Prunus persica). Acta Physiology Plant, 10, 37–81.Zhang D Q, Xu B H, Yang X H, Zhang Z Y, Li B L. 2011. The sucrose synthase gene family in Populus: Structure, expression, and evolution. Tree Genetics Genomes, 7, 443–456.Zhang J S, Arro J, Chen Y Q, Ming R. 2013. Haplotype analysis of sucrose synthase gene family in three Saccharum species. BMC Genomics, 14, 314.Zou C, Lu C, Shang H, Jing X, Cheng H, Zhang Y, Song G. 2013. Genome-wide analysis of the Sus gene family in cotton. Journal of Integrative Plant Biology, 55, 643–653.Zrenner R, Salanoubat M, Willmitzer L, Sonnewald U. 1995.Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum-tuberosuml). The Plant Journal, 7, 97–107. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
