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| In vitro investigation of the effect of dairy propionibacteria on rumen pH, lactic acid and volatile fatty acids |
| Jianbiao Luo1, Chaminda Senaka Ranadheera1, 2, Stuart King1, Craig Evans1, Surinder Baines3 |
1 School of Environmental & Life Sciences, The University of Newcastle, Callaghan NSW 2308, Australia
2 Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, Melbourne VIC 3030, Australia
3 School of Health Sciences, The University of Newcastle, Callaghan NSW 2308, Australia |
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Abstract Ruminal acidosis is a prevalent disorder in ruminants such as dairy cows and feedlot beef cattle, caused primarily by the inclusion of a high percentage of readily fermentable concentrates in the diet. The disorder presents as an accumulation of lactic acid, a decrease of pH in the rumen and a subsequent imbalance of the rumen fermentation process with detrimental impacts on the animal’s health and productivity. Dairy propionibacteria, a group of bacteria characterised by utilization of lactic acid as the favoured carbon source, with propionic acid produced as a by-product, were evaluated in this study as potential direct-fed microbials for use in controlling ruminal acidosis. Acidosis was simulated by introduction of high concentrations of lactic acid into rumen fluid samples and a multi-strain in vitro analysis was conducted, whereby changes in pH and lactic acid metabolism were compared in identical acidified rumen samples, following inoculation with various propionibacteria. This was followed by a study to evaluate the effect of bacterial inoculation dosage on acid metabolism. The results indicated that lactic acid levels in the rumen fluid were significantly reduced, and propionic acid and acetic acid concentrations both significantly increased, following addition of propionibacteria. Significant ‘between strains’ differences were observed, with Propionibacterium acidopropionici 341, Propionibacterium freudenreichii CSCC 2207, Propionibacterium jensenii NCFB 572 and P. jensenii 702 each producing more rapid reduction of lactic acid concentration than P. freudenreichii CSCC 2206, P. acidopropionici ATCC 25562 and Propionibacterium thoenii ATCC 4874. Furthermore, the efficacy of this application was dosage related, with the rates of reduction in lactic acid levels and production of propionic acid, both significantly greater for the higher (1010 cfu mL–1) compared with lower (105 cfu mL–1) dosage inoculation. The results confirmed that the introduction of propionibacteria could promote more rapid reduction of lactic acid levels than would occur without their addition, demonstrating their potential in controlling ruminal acidosis.
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Received: 01 June 2016
Accepted:
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| Fund: The University of Newcastle, Australia for financial support. |
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Corresponding Authors:
Correspondence Jianbiao Luo, Mobile: +61-0404724160, E-mail: bjastin@hotmail.com
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Cite this article:
Jianbiao Luo, Chaminda Senaka Ranadheera, Stuart King, Craig Evans, Surinder Baines .
2017.
In vitro investigation of the effect of dairy propionibacteria on rumen pH, lactic acid and volatile fatty acids. Journal of Integrative Agriculture, 16(07): 1566-1575.
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| Adams M C, Luo J, Rayward D, King S, Gibson R, Moghaddam G H. 2008. Selection of a novel direct-fed microbial to enhance weight gain in intensively reared calves. Animal Feed Science and Technology, 145, 41–52.Cousin F J, Mater D D, Foligné B, Jan G. 2011. Dairy propionibacteria as human probiotics: A review of recent evidence. Dairy Science & Technology, 91, 1–26.Dawson K A, Rasmussen M A, Allison M J. 1997. Digestive disorders and nutritional toxicity. In: Hobson P J, Stewart C S, eds., The Rumen Microbial Ecosystem. Blackie Academic & Professional, London. pp. 633–660.Dirksen G. 1985. The rumen acidosis complex - recent knowledge and experiences (1). A review. Tierarztliche Praxis, 13, 501–512.Elghandour M M, Salem A Z, Castañeda J S M, Camacho L M, Kholif A E, Chagoyán J C V. 2015. Direct-fed microbes: A tool for improving the utilization of low quality roughages in ruminants. Journal of Integrative Agriculture, 14, 526–533.Enemark J M D. 2008. The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review. Veterinary Journal, 176, 32–43.Enemark J M, Jorgensen R J, Kristensen N B. 2004. An evaluation of parameters for the detection of subclinical rumen acidosis in dairy herds. Veterinary Research Communications, 28, 687–709.Francisco C C, Chamberlain C S, Waldner D N, Wettemann R P, Spicer L J. 2002. Propionibacteria fed to dairy cows: Effects on energy balance, plasma metabolites and hormones, and reproduction. Journal of Dairy Science, 85, 1738–1751.Ghorbani G R, Morgavi D P, Beauchemin K A, Leedle J A. 2002. Effects of bacterial direct-fed microbials on ruminal fermentation, blood variables, and the microbial populations of feedlot cattle. Journal of Animal Science, 80, 1977–1985.Huang Y, Adams M C. 2004. In vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propionibacteria. International Journal of Food Microbiology, 91, 253–260.Huntington G B, Reynolds P J, Tyrrell H F. 1983. Net absorption and ruminal concentrations of metabolites in nonpregnant dry Holstein cows before and after intraruminal acetic acid infusion. Journal of Dairy Science, 66, 1901–1908.Hutton P G, Durmic Z, Ghisalberti E L, Flematti G R, Duncan R M, Carson C F, Riley T V. 2012. Inhibition of ruminal bacteria involved in lactic acid metabolism by extracts from Australian plants. Animal Feed Science and Technology, 176, 170–177.Kung L, Hession A O. 1995. Preventing in vitro lactate accumulation in ruminal fermentations by inoculation with Megasphaeraelsdenii. Journal of Animal Science, 73, 250–256.Koniarova I. 1993. Biochemical and physiologic properties of strains of Propionibacterium acnes isolated from the rumen of calves and lambs. Veterinarni Medicina, 38, 43–52.Lehloenya K V, Krehbiel C R, Mertz K J, Rehberger T G, Spicer L J. 2008. Effects of propionibacteria and yeast culture fed to steers on nutrient intake and site and extent of digestion. Journal of Dairy Science, 91, 653–662.Mackie R I, White B A. 1990. Recent advances in rumen microbial ecology and metabolism: Potential impact on nutrient output. Journal of Dairy Science, 73, 2971–2995.Martin S A, Streeter M N. 1995. Effect of malate on in vitro mixed ruminal microorganism fermentation. Journal of Animal Science, 73, 2141–2145.Nagaraja T G, Titgemeyer E C. 2007. Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook. Journal of Dairy Science, 90, E17–E38.Narvaez N, Alazzeh A Y, Wang Y, McAllister T A. 2014. Effect of Propionibacterium acidipropionici P169 on growth performance and rumen metabolism of beef cattle fed a corn- and corn dried distillers’ grains with solubles-based finishing diet. Canadian Journal Animal Science, 94, 363–369.Nocek J E, Kautz W P, Leedle J A, Allman J G. 2002. Ruminal supplementation of direct-fed microbials on diurnal pH variation and in situ digestion in dairy cattle. Journal of Dairy Science, 85, 429–433.Owens F N, Secrist D S, Hill W J, Gill D R. 1998. Acidosis in cattle: A review. Journal of Animal Science, 76, 275–286.Parrot T D, Rehberger T G, Owens F N. 2001. Selection of Propionibacterium strains capable of utilizing lactic acid from in vitro models. Journal of Animal Science, 1(Suppl.), 79–80.Pascal P. 1999. Metabolism of lactate and sugars by dairy propionibacteria: A review. Lait, 79, 23–41.Peters J P, Shen R Y, Robinson J A, Chester S T. 1990. Disappearance and passage of propionic acid from the rumen of the beef steer. Journal of Animal Science, 68, 3337–3349.Plaizier J C, Krause D O, Gozho G N, McBride B W. 2008. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. Veterinary Journal, 176, 21–31.Ranadheera C S, Evans C A, Adams M C, Baines S K. 2012. In vitro analysis of gastrointestinal tolerance and intestinal cell adhesion of probiotics in goat’s milk ice cream and yogurt. Food Research International, 49, 619–625.Ranadheera C S, Evans C A, Adams M C, Baines S K. 2014. Effect of dairy probiotic combinations on in vitro gastrointestinal tolerance, intestinal epithelial cell adhesion and cytokine secretion. Journal of Functional Foods, 8, 18–25. Raeth-Knight M L, Linn J G, Jung H G. 2007. Effect of direct-fed microbials on performance, diet digestibility, and rumen characteristics of Holstein dairy cows. Journal of Dairy Science, 90, 1802–1809.Seo J K, Kim S W, Kim M H, Upadhaya S D, Kam D K, Ha J K. 2010. Direct-fed microbials for ruminant animals. Asian-Australasian Journal of Animal Sciences, 23, 1657–1667.Stein D R, Allen D T, Perry E B, Bruner J C, Gates K W, Rehberger T G, Mertz K, Jones D, Spicer L J. 2006. Effects of feeding propionibacteria to dairy cows on milk yield, milk components, and reproduction. Journal of Dairy Science, 89, 111–125.Weiss W P, Wyatt D J, McKelvey T R. 2008. Effect of feeding propionibacteria on milk production by early lactation dairy cows. Journal of Dairy Science, 91, 646–652. |
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