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Journal of Integrative Agriculture  2016, Vol. 15 Issue (9): 2087-2096    DOI: 10.1016/S2095-3119(15)61233-3
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Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw
LIU Qin-hua1, LI Xiang-yu1, Seare T Desta1, ZHANG Jian-guo2, SHAO Tao1
1 Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.China
2 Department of Grassland Science, South China Agricultural University, Guangzhou 510642, P.R.China
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Abstract     The aim of the current study was to investigate the effects of a lactic acid bacteria inoculant (Lactobacillus plantarum, LP), fibrolytic enzyme (EN), combination of LP and EN (LP+EN) on fermentation quality, nutritive characteristics and in vitro digestibility of total mixed ration (TMR) silages containing 0, 7.5 and 15.0% (on dry matter basis) of rape (Brassica campestris L.) straw (RS) (denoted as CTMR, LTMR and MTMR, respectively).  After ensiling for 60 days, TMR silages without additives were well preserved, but MTMR had higher pH than CTMR and LTMR. There were no differences in other parameters of fermentation quality, microbial composition, nutrition and in vitro digestibility between CTMR and LTMR except for yeast and mold number and crude protein (CP) content. CTMR and LTMR silage had higher CP content, in vitro neutral detergent fiber digestibility (IVNDFD) and in vitro acid detergent fiber digestibility (IVADFD), lower acid detergent fiber (ADF) content than MTMR silage.  LP and EN decreased pH and increased dry matter (DM) recovery of TMR silages. LP+EN improved the fermentation quality, nutritive characteristics and in vitro digestibility of TMR silages, showed by lower pH, ADF content, higher lactic acid content, in vitro dry matter digestibility (IVDMD) and IVNDFD.  Therefore, It was suggested that TMR silage contained 7.5% RS on a DM basis and treated with LP+EN can be as a useful feed for ruminant.
Keywords:  fibrolytic enzyme        in vitro digestibility        lactic acid bacteria        silage        total mixed ration   
Received: 08 July 2015   Accepted:

The project was supported by the Natural Science Foundation of the Jiangsu Province for Young Scholars, China (BK20130694), the Specialized Research Fund for the Doctoral Program of Higher Education, China (20130097120053), the National Natural Science Foundation of China (31502014), and the Project of Jiangsu Independent Innovation, China (CX(15)1003).

Corresponding Authors:  SHAO Tao, Tel: +86-25-84396356, E-mail:   
About author:  LIU Qin-hua, Tel: +86-25-84396356, E-mail:;

Cite this article: 

LIU Qin-hua, LI Xiang-yu, Seare T Desta, ZHANG Jian-guo, SHAO Tao. 2016. Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw. Journal of Integrative Agriculture, 15(9): 2087-2096.

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