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Special Issue:
动物营养合辑Animal Nutrition
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The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| GUO Gang1*, SHEN Chen1*, LIU Qiang1 , ZHANG Shuan-lin1 , SHAO Tao2 , WANG Cong1 , WANG Yongxin1 , XU Qing-fang1 , HUO Wen-jie1 |
1 College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China
2 Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.China |
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Abstract The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mundtii (EM) for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility (DM-D), neutral detergent fiber digestibility (NDF-D), volatile fatty acids (VFA), methane (CH4 ) production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference (P>0.05) among the silage pH, lactic acid, crude protein (CP), water soluble carbohydrates (WSC) and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher (P<0.05) for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest (P<0.05) CH4 production per unit yield of VFA, which was positively corresponded to the lowest (P<0.05) ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum.
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Received: 27 November 2018
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31502015, 31672488) and the Natural Science Foundation of Shanxi Province of China (2015021162). |
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Corresponding Authors:
Correspondence HUO Wen-jie, Tel: +86-354-6287335, Fax: +86-354-6288052, E-mail:
huohuo1982@163.com
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| About author: GUO Gang, E-mail: gggg1984@163.com; SHEN Chen, E-mail:
shenchen63029@163.com; * These authors contributed equally to this study. |
Cite this article:
GUO Gang, SHEN Chen, LIU Qiang, ZHANG Shuan-lin, SHAO Tao, WANG Cong, WANG Yong-xin, XU Qing-fang, HUO Wen-jie.
2020.
The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage . Journal of Integrative Agriculture, 19(3): 838-847.
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Agarwal N, Kamra D N, Chaudhary L C, Agarwal I, Sahoo A, Pathak N N. 2002. Microbial status and rumen enzyme profile of crossbred calves fed ondifferent microbial feed additives. Letters in Applied Microbiology, 34, 329–336.
Amon T, Amon B, Kryvoruchko V, Machmüller A, Hopfner-Sixt K, Bodiroza V, Hrbek R, Friedel J, Pötsch E, Wagentristl H, Schreiner M, Zollitsch W. 2007. Methane production through anaerobic digestion of various energy crops grown in sustainable crop rotations. Bioresource Technology, 98, 3204–3212.
AOAC International (Association of Official Analytical Chemists). 2012. Official Methods of Analysis. 19th ed. AOAC International, Gaithersburg, MD.
Barker S B, Summerson W H. 1941. The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry, 138, 535?554.
Bayatkouhsar J, Tahmasbi A M, Naserian A A. 2012. Effects of microbial inoculant on composition, aerobic stability, in situ
ruminal degradability and in vitro gas production of corn silage. International Journal of Agriscience, 2, 774–786.
De Boever J L, Aerts J M, Vanacker J M, De Brabander D L. 2005. Evaluation of the nutritive value of maize silages using a gas production technique. Animal Feed Science and Technology, 123, 255–265.
Buxton D R, Muck R E, Harrison J H. 2003. Silage Science and Technology. ASA, CSSA and SSSA Publishers, Madison, WI.
Cai Y M. 1999. Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. Journal of Dairy Science, 82, 2466–2471.
Cao Y, Takahashia T, Horiguchia K, Yoshidaa N, Cai Y M. 2010. Methane emissions from sheep fed fermented or non-fermented total mixed ration containing whole-crop rice and rice bran. Animal Feed Science and Technology, 157, 72–78.
Catchpoole V R, Henzell E F. 1971. Silage and silage making from tropical herbage species. Herbage Abstracts, 41, 213–221.
Chen J, Stokes M R, Wallace C R. 1994. Effects of enzyme-inoculant systems on preservation and nutritive value of hay crop and corn silages. Journal of Dairy Science, 77, 501–512.
Contreras-Govea F E, Muck R E, Mertens D R, Weimer P J. 2011. Microbial inoculant effects on silage and in vitro ruminal fermentation, and microbial biomass estimation for alfalfa, bmr corn, and corn silages. Animal Feed Science and Technology, 163, 2–10.
Denman S E, McSweeney C S. 2006. Developmentofa real-time PCR assay formonitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology, 58, 572–582.
Desta S T, Yuan X J, Li J F, Shao T. 2016. Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives. Bioresource Technology, 221, 447–454.
Guo G, Yuan X J, Wen A Y, Liu Q, Zhang S L, Shao T. 2015. Silage fermentation characteristics of Napier grass (Pennisetum purpureum Sch.) harvested at various times on sunny day. Crop Science, 55, 458–464.
Herrmann C C, Idler C, Heiermann M. 2015. Improving aerobic stability and biogas production of maize silage using silage additives. Bioresource Technology, 197(Suppl. C), 393–403.
Hobson P N, Stewart C S. 1997. The Rumen Microbial Ecosystem. 2nd ed. Chapman and Hall, London, UK.
Hristov A N, Callaway T R, Lee C, Dowd S E. 2012. Rumen bacterial, archaeal, and fungal diversity of dairy cows in response to ingestion of lauric or myristic acid. Journal of Animal Science, 90, 4449–4457.
Johnson K A, Johnson D E. 1995. Methane emissions from cattle. Journal of Animal Science, 73, 2483–2492.
Kim S C, Adesogan A T. 2006. Influence of ensiling temperature, simulated rainfall, and delayed sealing on fermentation characteristics and aerobic stability of corn silage. Journal of Dairy Science, 89, 3122?3132.
Kleinschmit D H, Schmidt R J, Kung Jr L. 2005. The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. Journal of Dairy Science, 88, 2130?2139.
Koike S, Kobayashi Y. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus favefaciens. FEMS Microbiology Letters, 204, 361–366.
Kougias P G, Boe K, Tsapekos P, Angelidaki I. 2014. Foam suppression inoverloaded manure-based biogas reactors using antifoaming agents. Bioresource Technology, 153, 198–205.
Li H, Cao Y, Wang X M, Ge X, Li B Q, Jin C Q. 2017. Evaluation on the production of food crop straw in China from 2006 to 2014. Bioenergy Research, 10, 949–957.
Li J H, Zhang R H, Siddhu M A H, He Y F, Wang W, Li Y Q, Chen C, Liu G Q. 2015. Enhancing methane production of corn stover through a novel way: sequent pretreatment of potassium hydroxide and steam explosion. Bioresource Technology, 181, 345–350.
Lin C, Bolsen K K, Brent B E, Fung D Y C. 1992. Epiphytic lactic acid bacteria succession during the pre-ensiling and ensiling periods of alfalfa and maize. Journal of Applied Bacteriology, 73, 375–387.
Ma T, Chen D D, Tu Y, Zhang N F, Si B W, Deng K D, Diao Q Y. 2016. Effect of supplementation of allicin on methanogenesis and ruminal microbial flora in Dorper crossbred ewes. Journal of Animal Science and Biotechnology, 7, 1.
McDonald P, Edward R A, Greenhalgh J F D, Morgan C A, Sinclair L A, Wilkinson R G. 2011. Animal Nutrition. 7th ed. Pearson, Harlow, UK.
McDonald P, Henderson A R, Heron S J. 1991. The biochemistry of silage. 2nd ed. Cambrian Printers Ltd. Aberystwyth, UK.
Mehmood K, Chang S C, Yu S C, Wang L Q, Li P F, Li Z, Liu W P, Rosenfeld D, Seinfeld J H. 2018. Spatial and temporal distributions of air pollutant emissions from open crop straw and biomass burnings in China from 2002 to 2016. Environmental Chemistry Letters, 16, 301–309.
Menke K H, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55.
Moore K J, Moser L E. 1995. Quantifying developmental morphology of perennial grasses. Crop Science, 35, 37–43.
Muck R E, Filya I, Contreras-Govea F E. 2007. Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production. Journal of Dairy Science, 90, 5115–5125.
Navarro-Villa A, O’Brien M, López S, Boland T M, O’Kiely P. 2011. In vitro rumen methane output of red clover and perennial ryegrass assayed using the gas production technique (GPT). Animal Feed Science and Technology, 168, 152?164.
Navarro-Villa A, O’Brien M, López S, Boland T M, O’Kiely P. 2013. In vitro rumen methane output of grasses and grass silages differing in fermentation characteristics using the gas-production technique (GPT). Grass and Forage Science, 68, 228?244.
Ørskov E R, McDonald I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science, 92, 499–503.
Pei C X, Liu Q, Dong C S, Li H Q, Jiang J B, Gao W J. 2013. Microbial community in the forestomachs of alpacas (Lama pacos) and sheep (Ovis aries). Journal of Integrative Agriculture, 12, 314–318.
SAS. 1999. SAS/STAT user’s guide. version 8.01th ed. SAS Institute Inc., Cary, North Carolina, USA.
Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583?3597.
Wang J Z, Wang X J, Xu M G, Feng G, Zhang W J, Lu C A. 2015. Crop yield and soil organic matter after long-term straw return to soil in China. Nutrient Cycling in Agroecosystems, 102, 371–381.
Wang R F, Cao W W, Cerniglia C E. 1997. PCR detection of Ruminococcus spp. in human and animal faecal samples. Molecular and Cellular Probes, 11, 259–265.
Weinberg Z G, Chen Y, Gamburg M. 2004. The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. Journal of Dairy Science, 87, 3386–3397.
Weinberg Z G, Shatz O, Chen Y, Yosef E, Nikbahat M, Ben-Ghedalia D, Miron J. 2007. Effect of lactic acid bacteria inoculants on in vitro digestibility of wheat and corn silages. Journal of Dairy Science, 90, 4754–4762.
Zhang J G, Kawamoto H, Cai Y M. 2010. Relationships between the addition rates of ellulose or glucose and silage fermentation at different temperatures. Animal Science Journal, 81, 325–330.
Zhang Q, Li X, Zhao M, Yu Z. 2016. Lactic acid bacteria strains for enhancing the fermentation quality and aerobic stability of Leymus chinensis silage. Grass and Forage Science, 71, 472–481.
Zoetendal E G, Akkermans A D L, De Vos W M. 1998. Temperature gradient gel electrophoresis analysis of 16S rRNA from human faecal samples reveals stable and host-specific communities of active bacteria. Applied and Environmental Microbiology, 64, 3854?3859.
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