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Journal of Integrative Agriculture  2020, Vol. 19 Issue (3): 838-847    DOI: 10.1016/S2095-3119(19)62707-3
Special Issue: 动物营养合辑Animal Nutrition
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage
GUO Gang1*, SHEN Chen1*, LIU Qiang1 , ZHANG Shuan-lin1 , SHAO Tao2 , WANG Cong1 , WANG Yongxin1 , XU Qing-fang1 , HUO Wen-jie1
1 College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China
2 Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.China
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Abstract  The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mundtii (EM) for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility (DM-D), neutral detergent fiber digestibility (NDF-D), volatile fatty acids (VFA), methane (CH4 ) production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference (P>0.05) among the silage pH, lactic acid, crude protein (CP), water soluble carbohydrates (WSC) and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher (P<0.05) for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest (P<0.05) CH4 production per unit yield of VFA, which was positively corresponded to the lowest (P<0.05) ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum.
Keywords:  corn stover        in vitro digestibility        lactic acid bacteria        silage  
Received: 27 November 2018   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31502015, 31672488) and the Natural Science Foundation of Shanxi Province of China (2015021162).
Corresponding Authors:  Correspondence HUO Wen-jie, Tel: +86-354-6287335, Fax: +86-354-6288052, E-mail:    
About author:  GUO Gang, E-mail:; SHEN Chen, E-mail:; * These authors contributed equally to this study.

Cite this article: 

GUO Gang, SHEN Chen, LIU Qiang, ZHANG Shuan-lin, SHAO Tao, WANG Cong, WANG Yong-xin, XU Qing-fang, HUO Wen-jie. 2020.

The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage
. Journal of Integrative Agriculture, 19(3): 838-847.

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