QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

doi:10.1016/S2095-3119(16)61361-8

Journal of Integrative Agriculture

2017, Vol. 16

Issue (02): 435-444 DOI: 10.1016/S2095-3119(16)61361-8

Crop Genetics · Breeding · Germplasm Resources

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QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

ZHOU Sheng-hui¹, FU Lin¹, WU Qiu-hong², CHEN Jiao-jiao¹, CHEN Yong-xing¹, XIE Jing-zhong¹, WANG Zhen-zhong³, WANG Guo-xin¹, ZHANG De-yun¹, LIANG Yong¹, ZHANG Yan¹, YOU Ming-shan¹, LIANG Rong-qi¹, HAN Jun⁴, LIU Zhi-yong^{1, 2}

¹State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China

²Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China

³China Rural Technology Development Center, Beijing 100045, P.R.China

⁴College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, P.R.China

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Abstract Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population of Yanda 1817×Beinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A.1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD≥2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29–21.65% and 4.36–5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS).

Keywords: wheat pre-harvest sprouting quantitative trait loci SNP TaVp-1A

Received: 01 February 2016 Accepted:

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This work was financially supported by the National Natural Science Foundation of China (31271710, 31301312).

Corresponding Authors: LIU Zhi-yong, Tel: +86-10-64806422, E-mail: zyliu@genetics.ac.cn

About author: ZHOU Sheng-hui, E-mail: zhoushenghui826@gmail.com

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	ZHOU Sheng-hui
	FU Lin
	WU Qiu-hong
	CHEN Jiao-jiao
	CHEN Yong-xing
	XIE Jing-zhong
	WANG Zhen-zhong
	WANG Guo-xin
	ZHANG De-yun
	LIANG Yong
	ZHANG Yan
	OU Ming-shan
	LIANG Rong-qi
	HAN Jun
	LIU Zhi-yong

Cite this article:

ZHOU Sheng-hui, FU Lin, WU Qiu-hong, CHEN Jiao-jiao, CHEN Yong-xing, XIE Jing-zhong, WANG Zhen-zhong, WANG Guo-xin, ZHANG De-yun, LIANG Yong, ZHANG Yan, OU Ming-shan, LIANG Rong-qi, HAN Jun, LIU Zhi-yong. 2017. QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6. Journal of Integrative Agriculture, 16(02): 435-444.

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