Creation of gene-specific rice mutants by AvrXa23-based TALENs

doi:10.1016/S2095-3119(16)61411-9

Journal of Integrative Agriculture

2017, Vol. 16

Issue (02): 424-434 DOI: 10.1016/S2095-3119(16)61411-9

Crop Genetics · Breeding · Germplasm Resources

Advanced Online Publication | Current Issue | Archive | Adv Search

Creation of gene-specific rice mutants by AvrXa23-based TALENs

WANG Fu-jun^{1, 2*}, WANG Chun-lian^1*, ZHENG Chong-ke¹, QIN Teng-fei¹, GAO Ying¹, LIU Pi-qing², ZHAO Kai-jun¹

¹National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, P.R.China

²College of Agriculture, Guangxi University, Nanning 530004, P.R.China

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Abstract Transcription activator-like effector (TALE) nucleases (TALENs) are increasingly used as a powerful tool for genome editing in a variety of organisms. We have previously cloned the TALE-coding gene avrXa23 from Xanthomonas oryzae pv. oryzae and developed an AvrXa23-based assembly system for designer TALEs or TALENs. Here, we exploit TALENs to induce mutagenesis of the rice ethylene response factor (ERF) transcription factor OsERF922 for testing the gene-editing efficiency of AvrXa23-based TALENs system. A pair of TALENs (T-KJ9/KJ10) was assembled and their nuclease activities were first confirmed in rice protoplast transient assay. The TALENs-expressing construct pT-KJ9/KJ10 was then used for rice transformation. We observed targeting somatic mutagenesis frequency of 15.0% in positive transgenic rice calli and obtained two mutant plants with nucleotide deletion or insertion at the designer target region. Our work demonstrates that the AvrXa23-based TALENs system can be used for site-specific genome editing in rice.

Keywords: TAL effector nucleases AvrXa23 targeting OsERF922 genome editing rice

Received: 23 January 2016 Accepted:

Fund:

This work was supported by the grant from the Major Science and Technology Project to Create New Crop Cultivars Using Gene Transfer Technology of China (2014ZX0801001B) and the National Natural Science Foundation of China (31171812).

Corresponding Authors: ZHAO Kai-jun Tel: +86-10-82105852, Fax: 86-10-82108751, E-mail: zhaokaijun@caas.cn; LIU Pi-qing, Mobile: +86-13397700471, E-mail: liupq@gxu.edu.cn

About author: WANG Fu-jun, Tel: +86-10-82105852, E-mail: wej129@163.com; WANG Chun-lian, Tel: +86-10-82105852, E-mail: wangchunlian @caas.cn

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	WANG Fu-jun
	WANG Chun-lian
	ZHENG Chong-ke
	QIN Teng-fei
	GAO Ying
	LIU Pi-qing
	ZHAO Kai-jun

Cite this article:

WANG Fu-jun, WANG Chun-lian, ZHENG Chong-ke, QIN Teng-fei, GAO Ying, LIU Pi-qing, ZHAO Kai-jun . 2017. Creation of gene-specific rice mutants by AvrXa23-based TALENs. Journal of Integrative Agriculture, 16(02): 424-434.

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