|
|
|
Expression pattern and function analyses of the MADS thranscription factor genes in wheat (Triticum aestivum L.) under phosphorusstarvation condition |
SHI Shu-ya, ZHANG Fei-fei, GAO Si, XIAO Kai |
Key Laboratory of Crop Growth Regulation of Hebei Province/College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China |
|
|
Abstract MADS-box (MADS) transcription factors (TFs) act as one of the largest TF families in plants. The members in this family play fundamental roles in almost every developmental process as well as involve plant responses to biotic and abiotic stresses. In this study, 54 of MADS genes in wheat, including 31 released publicly and 23 deposited as tentative consensus (TC) into GenBank database, were subjected to analyses of molecular characterization, expression pattern, and function under contrasting phosphate (Pi)-supply conditions. The 31 released MADS genes share cDNA full lengths of 683 to 1 297 bp, encoding amino acids of 170 to 274 aa that possess molecular weights of 19.21 to 31.33 kDa and isoelectric points of 5.74 to 9.63. Phylogenetic analysis categoried these wheat MADS genes into four subgroups containing 11, 5, 10, and 4 members, respectively. Under Pi sufficiency, the MADS genes showed drastically varied transcripts and they were categoried into expression groups of high, medium, low, and very low, respectively. Among them, several ones were differentially expressed under Pi deprivation, including that five were upregulated (TaMADS51, TaMADS4, TaMADS5, TaMADS6, and TaMADS18) and four were downregulated (TaMADAGL17, TaMADAGL2, TaMADWM31C, and TaMADS;14). qPCR analyses confirmed their expression patterns in responding to the Pi-starvation stress. TaMADS51, one of the upregulated genes by Pi deprivation, was subjected to the functional analysis in mediating plant tolerance to the Pi-starvation stress. The transgenic tobocco plants overexpressing TaMADS51 exhibited much more improved growth features, drymass, Pi acquisition, and photosynthetic parameters as well as antioxidant enzymatic activities under Pi deprivation than wild type. These results indicate that distinct MADS genes are transcriptional response to Pi deprivation and play critical roles in mediating plant tolerance to this stressor through regulating downstream Pi-responsive genes.
|
Received: 03 June 2015
Accepted:
|
Fund: This work was supported by the National Natural Science Foundation of China (31201674 and 31371618), the National Transgenic Major Program, China (2011ZX08008). |
Corresponding Authors:
XIAO Kai, Tel: +86-312-7520153, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn
|
Cite this article:
SHI Shu-ya, ZHANG Fei-fei, GAO Si, XIAO Kai.
2016.
Expression pattern and function analyses of the MADS thranscription factor genes in wheat (Triticum aestivum L.) under phosphorusstarvation condition. Journal of Integrative Agriculture, 15(8): 1703-1715.
|
Arora R, Agarwal P, Ray S, Singh A K, Singh V P, Tyagi A K, Kapoor S. 2007. MADS-box gene family in rice: Genome-wide identification, organization and expression profiling during reproductive development and stress. BMC Genomics, 8, 242.Cordell D, Drangert J O, White S. 2009. The story of phosphorus: Global food security and food for thought. Global Environmental Change, 19, 292–305.De Bodt S, Raes J, Florquin K, Rombauts S, Rouze P, Theissen G, Van de Peer Y. 2003. Genomewide structural annotation and evolutionary analysis of the type I MADS-box genes in plants. Journal of Molecular Evolution, 56, 573–586. Du H, Yang S S, Liang Z, Feng B R, Liu L, Huang Y B, Tang Y X. 2012. Genome-wide analysis of the MYB transcription factor superfamily in soybean. BMC Plant Biology, 12, 106.Glassop D, Smith S E, Smith F W. 2005. Cereal phosphate transporters associated with the mycorrhizal pathway of phosphate uptake into roots. Planta, 222, 688–698.Guo C, Zhao X, Liu X, Zhang L, Gu J, Li X, Lu W, Xiao K. 2013 Function of wheat phosphate transporter gene TaPHT2;1 in Pi translocation and plant growth regulation under replete and limited Pi supply conditions. Planta, 237, 1163–1178.Gupta S K, Rai A K, Kanwar S S, Chand D, Singh N K, Sharma T R. 2012. The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice. Journal of Experimental Botany, 63, 757–772.Hileman L C, Sundstrom J F, Litt A, Chen M, Shumba T, Irish V F. 2006. Molecular and phylogenetic analyses of the MADS-box gene family in tomato. Molecular Biology and Evolution, 23, 2245–2258. Kaufmann K, Melzer R, Theißen G. 2005. MIKC-type MADS-domain proteins: Structural modularity, protein interactions and network evolution in land plants. Gene, 347, 183–198.Liu X, Zhao X, Zhang L, Lu W, Li X, Xiao K. 2013. TaPht1;4, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), plays an important role in plant phosphate acquisition under phosphorus deprivation. Functional Plant Biology, 40, 329–341. Li X, Guo C, Gu J, Duan W, Zhao M, Ma C, Du X, Lu W, Xiao K. 2014a. Overexpression of TaVP, a vacuolar H+-pyrophosphatase gene in wheat (Triticum aestivum L.), improves tobacco plant growth under Pi and N deprivations, high salinity, and drought. Journal of Experimental Botany, 65, 683–696.Li X, Guo C, Lu W, Duan W, Zhao M, Ma C, Gu J, Xiao K. 2014b. Expression pattern analysis of zinc finger protein genes in wheat (Triticum aestivum L.) under phosphorus deprivation. Journal of Integrative Agriculture, 13, 1621–1633.Misson J, Raghothama K G, Jain A, Jouhet J, Block M A, Bligny R, Ortet P, Creff A, Somerville S, Rolland N. 2005. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proceedings of the National Academy of Sciences of the United States of America, 102, 11934–11939.Nam J, Kim J, Lee S, An G, Ma H, Nei M. 2004. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proceedings of the National Academy of Sciences of the United States of America, 101, 1910–1915.Nussaume L, Kanno S, Javot H, Marin E, Pochon N, Ayadi A, Nakanishi T M, Thibaud M C. 2011. Phosphate import in plants: Focus on the PHT1 transporters. Frontiers in Plant Science, 13, 83.Parenicova L, de Folter S, Kieffer M, Horner D S, Favalli C, Busscher J, Cook H E, Ingram R M, Kater M M, Davies B, Angenent G C, Colombo L. 2003. Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: New openings to the MADS world. The Plant Cell, 15, 1538–1551.Rubio V, Linhares F, Solano R, Martín A C, Iglesias J, Leyva A, Paz-Ares J. 2001. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Gene Development, 15, 2122–2133.Schwarz-Sommer Z, Huijser P, Nacken W, Saedler H, Sommer H. 1990. Genetic control of flower development by homeotic genes in Antirrhinum majus. Science, 250, 931–936. Smaczniak C, Immink R G, Angenent G C, Kaufmann K. 2012. Developmental and evolutionary diversity of plant MADS-domain factors: Insights from recent studies. Development, 139, 3081–3098. Sun Z, Ding C, Li X, Xiao K. 2012. Molecular characterization and expression analysis of TaZFP15, a C2H2-type zinc finger transcription factor gene in wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 11, 31–42.Vance C P, Uhde-stone C, Allan D L. 2003. Phosphorus acquisition and use: Critical adaptations by plants for securing a non renewable resource. New Phytologist, 157, 423–427.Verelst W, Saedler H, Munster T. 2007a. MIKC* MADS-protein complexes bind motifs enriched in the proximal region of late pollen-specific Arabidopsis promoters. Plant Physiology, 143, 447–460.Verelst W, Twell D, de Folter S, Immink R, Saedler H, Münster T. 2007b. MADS-complexes regulate transcriptome dynamics during pollen maturation. Genome Biology, 8, R249. Yanofsky M F, Ma H, Bowman J L, Drews G N, Feldmann K A, Meyerowitz E M. 1990. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature, 346, 35–39. Yoshida H, Nagato Y. 2011. Flower development in rice. Journal of Experimental Botany, 62, 4719–4730. Zhao Y, Li X, Chen W, Peng X, Cheng X, Zhu X, Cheng B. 2010. Whole-genome survey and characterization of MADS-box gene family in maize and sorghum. Plant Cell Tissue and Organ Culture, 105, 159–173. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|