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Journal of Integrative Agriculture  2021, Vol. 20 Issue (8): 2138-2155    DOI: 10.1016/S2095-3119(20)63313-5
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Genome-wide identification and expression analysis of Argonaute gene family from longan embryogenic callus
CHEN Rong-zhu1, 2, SHEN Xu1, ZHANG Shu-ting1, ZHAO Hua1, CHEN Xiao-hui1, XU Xiao-ping1, HUO Wen1, ZHANG Zi-hao1, LIN Yu-ling1, LAI Zhong-xiong1
1 Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
2 Department of Pharmacy, Zhangzhou Health Vocational College, Zhangzhou 363000, P.R.China
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Argonaute (AGO) proteins are the core of the RNA-induced gene silencing complex which regulate a wide variety of processes in plants, from organ development to abiotic stress responses.  They have been identified in many plants, but little is known in longan (Dimocarpus longan Lour.), and how AGO functions in the signaling pathways in plant embryos in response to changing environmental stimuli remains unclear.  In the present research, a genome-wide analysis of the AGO gene family members and their roles in somatic embryogenesis (SE), zygotic embryogenesis (ZE), tissue developmental processes, and responses to hormones, light and abiotic stress in longan were conducted.  Ten longan AGO genes were identified genome-wide and divided into four clades.  They were distributed on chromosomes 1, 4, 8, 10, 12, 13, 14, and 15, and had 2–23 introns.  The expression profiling implied that DlAGOs regulated early and middle embryogenesis, as well as developmental processes of seed, flower, and stem in longan.  In addition, the transcript levels of DlAGOs in response to exogenous hormones, light and abiotic stress showed differences in expression patterns.  These results provide the useful information for further elucidation of RNAi-mediated gene silencing in longan embryogenic callus (EC).
Keywords:  Argonaute family        longan embryogenesis        expression profile        hormones        stress response  
Received: 07 April 2020   Accepted:
Fund: This study was supported by the National Natural Science Foundation of China (31572088 and 31672127), the Science and Technology Plan Major Projects of Fujian Province of China (2015NZ00021), the New Century Excellent Talents Support Program in Fujian Province University of China (20151104), and the Special Fund for Scientific and Technological Innovation of Fujian Agricultural and Basic Research of China (CXZX2017314).
Corresponding Authors:  Correspondence LAI Zhong-xiong, E-mail:   
About author:  CHEN Rong-zhu, E-mail:;

Cite this article: 

CHEN Rong-zhu, SHEN Xu, ZHANG Shu-ting, ZHAO Hua, CHEN Xiao-hui, XU Xiao-ping, HUO Wen, ZHANG Zi-hao, LIN Yu-ling, LAI Zhong-xiong. 2021. Genome-wide identification and expression analysis of Argonaute gene family from longan embryogenic callus. Journal of Integrative Agriculture, 20(8): 2138-2155.

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