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Journal of Integrative Agriculture  2021, Vol. 20 Issue (8): 2204-2213    DOI: 10.1016/S2095-3119(20)63297-X
Special Issue: 昆虫合辑Plant Protection—Entomolgy 昆虫分子生物学合辑Insect Molecular Biology
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Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera: Coccinellidae)
QU Cheng1, 2, WANG Ran2, CHE Wu-nan3, LI Feng-qi2, ZHAO Hai-peng1, WEI Yi-yun2, LUO Chen2, XUE Ming
1 College of Plant Protection, Shandong Agricultural University, Tai’an 271018, P.R.China
2 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China
3 Department of Pesticide Sciences, Shenyang Agricultural University, Shenyang 110866, P.R.China
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昆虫嗅觉系统在其寻找寄主、交配和产卵等行为中发挥重要作用。气味结合蛋白(OBPs)参与化学信息素的识别,OBPs识别结合气味分子并将其运送至嗅觉神经元上的气味受体。异色瓢虫(鞘翅目:瓢甲科)作为重要的生防天敌被广泛用于防治多种农林业害虫。本研究中,从异色瓢虫成虫触角和整虫转录组中共鉴定到19个OBP基因,所有OBP基因均具有全长开放阅读框,其编码蛋白中11个属于‘Classic’OBPs,7 个属于‘Minus-C’OBPs ,1 个属于‘Plus-C’OBP。它们编码125-241个氨基酸,相对分子量为13.74KDa-27.75 KDa,等电点范围为4.15-8.80。利用系统发育分析研究了异色瓢虫气味结合蛋白与其他鞘翅目昆虫气味结合蛋白的关系。荧光定量PCR结果表明HaxyOBP2,3,5,8,10,12,13,14和15在雌雄成虫触角中高表达;其中HaxyOBP2,3,5,12和15在触角中的表达量显著高于其他组织,HaxyOBP13HaxyOBP14在触角和头部的表达量相当,而其他基因在胸、腹、足和翅等非嗅觉组织中高表达。本研究结果为进一步探究异色瓢虫嗅觉系统提供了有价值信息,并将增强异色瓢虫作为生防天敌的应用效果。

The olfactory system of insects is crucial in modulating behaviors such as host seeking, mating, and oviposition.  Odorant-binding proteins (OBPs) are involved in semiochemical recognition.  OBPs recognize and bind odorants and transport them to odorant receptors located in olfactory neurons.  Harmonia axyridis (Coleoptera: Coccinellidae) is a widely used predacious biological control agent for many agricultural and forestry pests.  This study identified 19 OBPs in H.?axyridis based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames, including 11 ‘Classic’ OBPs, 7 ‘Minus-C’ OBPs and 1 ‘Plus-C’ OBP.  They encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to 8.80.  Phylogenetic analyses were used to study the relationships between H.?axyridis OBPs and OBPs from other species of Coleoptera.  Quantitative real-time PCR (qPCR) analysis showed that HaxyOBP2, 3, 5, 8, 10, 12, 13, 14, and 15 were highly expressed in antennae of both adult females and males.  Moreover, HaxyOBP2, 3, 5, 12, and 15 were more abundantly expressed in antennae than other body parts, while HaxyOBP13 and HaxyOBP14 were expressed predominantly, and at similar levels, in the head and antennae.  The other OBP genes were highly expressed in non-olfactory tissues including the thorax, abdomen, legs, and wings.  These results provide valuable information for further study of H.?axyridis olfaction, which may ultimately enhance its use as a biocontrol agent.
Keywords:  odorant-binding proteins        Harmonia axyridis        expression profile        phylogenetic tree  
Received: 14 April 2020   Accepted:
Fund: This work was supported by the National Key Research and Develop Program of China (2017YFD0200400), the Shandong Province Modern Agricultural Technology System Peanut Innovation Team, China (SDAIT-04-08) and the Beijing Leafy Vegetables Innovation Team of Modern Agro-industry Technology Research System, China (BAIC07-2020).
Corresponding Authors:  Correspondence XUE Ming, Tel: +86-538-8249349, E-mail:; LUO Chen, Tel: +86-10-51503338, E-mail:    
About author:  QU Cheng, Tel: +86-10-51505278, E-mail:;

Cite this article: 

QU Cheng, WANG Ran, CHE Wu-nan, LI Feng-qi, ZHAO Hai-peng, WEI Yi-yun, LUO Chen, XUE Ming. 2021. Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera: Coccinellidae). Journal of Integrative Agriculture, 20(8): 2204-2213.

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