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| Ligand-binding properties of three odorant-binding proteins of the diamondback moth Plutella xylostella |
| ZHU Jiao, Paolo Pelosi, LIU Yang, LIN Ke-jian, YUAN Hai-bin, WANG Gui-rong |
1、College of Agriculture, Jilin Agricutural University, Jilin 130118, P.R.China
2、State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of
Agricultural Sciences, Beijing 100193, P.R.China |
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摘要 Strategies for insect population control are currently targeting chemical communication at the molecular level. The diamondback moth Plutella xylostella represents one of the most serious pests in agriculture, however detailed information on the proteins mediating olfaction in this species is still poor. This species is endowed with a repertoire of a large number of olfactory receptors and odorant binding proteins (OBPs). As a contribution to map the specificities of these chemical sensors in the moth and eventually unravel the complexity of chemodetection, we have measured the affinities of three selected OBPs to a series of potential odorants. Three proteins are highly divergent in their amino acid sequences and show markedly different expression profiles. In fact, PxylOBP3 is exclusively expressed in the antennae of both sexes, PxylOBP9 is male specific and present only in antennae and reproductive organs, while PxylOBP19, an unusual OBP with nine cysteines, is ubiquitously present in all the organs examined. Such expression pattern suggests that the last two proteins may be involved in non-chemosensory functions. Despite such differences, the three OBPs exhibit similar binding spectra, together with high selectivity. Among the 26 natural compounds tested, only two proved to be good ligands, retinol and coniferyl aldehyde. This second compound is particularly interesting being part of the chemical pathway leading to regeneration of lignin, one of the defense strategies of the plant against insect attack, and might find applications as a repellent for P. xylostella and other pests.
Abstract Strategies for insect population control are currently targeting chemical communication at the molecular level. The diamondback moth Plutella xylostella represents one of the most serious pests in agriculture, however detailed information on the proteins mediating olfaction in this species is still poor. This species is endowed with a repertoire of a large number of olfactory receptors and odorant binding proteins (OBPs). As a contribution to map the specificities of these chemical sensors in the moth and eventually unravel the complexity of chemodetection, we have measured the affinities of three selected OBPs to a series of potential odorants. Three proteins are highly divergent in their amino acid sequences and show markedly different expression profiles. In fact, PxylOBP3 is exclusively expressed in the antennae of both sexes, PxylOBP9 is male specific and present only in antennae and reproductive organs, while PxylOBP19, an unusual OBP with nine cysteines, is ubiquitously present in all the organs examined. Such expression pattern suggests that the last two proteins may be involved in non-chemosensory functions. Despite such differences, the three OBPs exhibit similar binding spectra, together with high selectivity. Among the 26 natural compounds tested, only two proved to be good ligands, retinol and coniferyl aldehyde. This second compound is particularly interesting being part of the chemical pathway leading to regeneration of lignin, one of the defense strategies of the plant against insect attack, and might find applications as a repellent for P. xylostella and other pests.
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Received: 12 February 2015
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31230062 and 31321004), the Beijing Natural Science Foundation of China (6132028) and the State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences (SKLOF201502). |
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Corresponding Authors:
WANG Gui-rong, Tel: +86-10-62816947,E-mail: grwang@ippcaas.cn; YUAN Hai-bin,
E-mail: yhb-74@163.com
E-mail: grwang@ippcaas.cn;yhb-74@163.com
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Cite this article:
ZHU Jiao, Paolo Pelosi, LIU Yang, LIN Ke-jian, YUAN Hai-bin, WANG Gui-rong.
2016.
Ligand-binding properties of three odorant-binding proteins of the diamondback moth Plutella xylostella. Journal of Integrative Agriculture, 15(3): 580-590.
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