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Journal of Integrative Agriculture  2015, Vol. 14 Issue (10): 1971-1979    DOI: 10.1016/S2095-3119(15)61049-8
Physiology·Biochemistry·Cultivation·Tillage Advanced Online Publication | Current Issue | Archive | Adv Search |
The effects of high temperature level on square Bt protein concentration of Bt cotton
 WANG  Jun, CHEN  Yuan, YAO  Meng-hao, LI  Yuan, WEN  Yu-jin, CHEN  Yuan, ZHANG  Xiang, CHEN  De-hua
1、Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
2、Department of Soil and Crop Sciences, Texas A&M University, College Station, TX77843, USA
3、Syngenta Biotechnology Inc., Carry, NC 27519, USA
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摘要  Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature >38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic- pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is (>38°C), the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.

Abstract  Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature >38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic- pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is (>38°C), the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.
Keywords:  Bt cotton       square       high temperature level       stress       Bt protein       nitrogen metabolism  
Received: 26 January 2015   Accepted:
Fund: 

This work was supported by the National Natural Science Foundation of China (31171479, 31301263 and 31471435), the Doctoral Advisor Foundation of Ministry of Education of China (20113250110001), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), and the Three New Technology Foundation of Agriculture in Jiangsu Province, China (SXGC(2014)317).

Corresponding Authors:  CHEN De-hua, Tel: +86-514-87979357,Fax: +86-514-87996817, E-mail: cdh@yzu.edu.cn     E-mail:  cdh@yzu.edu.cn
About author:  WANG Jun, E-mail: 505417379@qq.com;

Cite this article: 

WANG Jun, CHEN Yuan, YAO Meng-hao, LI Yuan, WEN Yu-jin, CHEN Yuan, ZHANG Xiang, CHEN De-hua. 2015. The effects of high temperature level on square Bt protein concentration of Bt cotton. Journal of Integrative Agriculture, 14(10): 1971-1979.

Adamczyk J J, Meredith W R. 2004. Genetic basis for thevariability of CryIAc expression among commercialtransgenic Bacillus thuringiensis (Bt) cotton cultivars in theUnited States. Journal of Cotton Science, 8, 17-23

Benedict J H, Sachs E S, Altman D W, Deaton W R, Kohel RJ, Ring D R, Berberich S A. 1996. Field performance ofcottons expressing transgenic CryIA insecticidal proteins for resistance to Heliothisvirescens and Helicoverpazea(Lepidoptera: Noctuidae). Journal of Economic Entomology,89, 230-238

Bradford M M. 1976. A rapid and sensitive method for thequantification of microgram quantities of protein utilizingthe principle of protein-dye binding. Analytical Biochemistry,72, 248-254

Chen D H, Ye G Y, Yang C Q, Chen Y, Wu Y K. 2005. Theeffect of the hightemperature on the insecticidal propertiesof the cotton. Environmental and Experimental Botany,53, 333-342

Chen S, Wu J Y, He X L, Huang J Q, Zhou B L, Zhang R X.1997. Quantification using ELISA of Bacillus thuringiensisinsecticidal protein expressed in the tissue of transgenicinsect-resistant cotton. Journal of Jiangsu AgricultureScience, 3, 154-156 (in Chinese)

Chen Y, Wen Y, Chen Y, Cothren J T, Zhang X, Wang Y, PayneW A, Chen D H. 2012. Effects of extreme air temperatureand humidity on the insecticidal expression level of Btcotton. Journal of Integrative Agriculture, 11, 101-108

Clive J. 2012. The development state for commercialBiotechnology and transgenic crops. China Biotechnology,32, 1-14

Cui J J, Xia J Y. 1999. Studies on the resistance dynamics ofthe Bt transgenic cotton on cottonbollworm. Acta GossipiSinica, 11, 141-146

Chen D H, Ye G Y, Yang C Q, Chen Y, Wu Y K. 2004. Effectof introducing Bacillus thuringiensis gene on nitrogenmetabolism in cotton. Field Crops Research, 87, 235-244

Dong Z Q, He Z P, Zhai X J. 2000. The metabolic charactersof nitrogen in leaves of Bt transgenic cotton Nucoton 33Band its regulation. Cotton Science, 3, 113-117 (in Chinese)

Fitt G P. 1998. Efficacy of Ingard® cotton-patterns andconsequences. In: The Ninth Australian Cotton ConferenceProceedings. The Cotton Research & DevelopmentCorporation, Australia. pp. 233-245

Glenn D S. 2011. Field versus farm warangal: Btcotton,highyields, and larger questions. World Development, 3,387-398

Greenplate J T, Penn S R, Mullins J W, Oppenhuizen M. 2000.Seasonal CryIAc levels in DP50B: The “Bollgard® basis”for Bollgard II. In: Dugger P, Richter D, eds., Belt WideCotton Conference Proceedings. National Cotton Council,Memphis. pp. 1039-1040

Hallikeri S S, Halemani H L, Katageri I S, Patil B C, PatilV C, Palled Y B. 2009. Influence of sowing time andmoisture regimes on cry protein concentration and relatedparameters of Bt-cotton. Karnataka Journal of AgricultureSciences, 22, 995-1000

Huang J K, Mi J W, Lin H, Wang Z J, Chen R J, Hu R F, ScottR, Carl P. 2010. A decade of Bt cotton in Chinese field:Assessing direct effect and indirect externalities of Btcotton adoption in China. Science China (Life Science),53, 981-991

Ian J R. 2006. Effect of genotype, edaphic, environmentalconditions, andagronomic practices on Cry1Ac proteinexpression in transgenic cotton. The Journal of CottonScience, 10, 252-262

Kaiser J. 1996. Pest overwhelm Bt cotton crop. Science, 26,423-423

Mo H D. 1983. Agricultural Experiment Statistics. ShanghaiScience and Technology Press, Shanghai. (in Chinese)

Olsen K M, Daly J C. 2000. Plant-toxin interactions intransgenic Bt cotton and their effect on mortality ofHelicoverpaarmigera (Lepidoptera: Noctuidae). Journal ofEconomic Entomology, 4, 1293-1299

Olsen K M, Daly J C, Finnegan E J, Mahon R J. 2006.Changes in Cry1AcBt transgenic cotton in response to twoenvironmental factors: temperature and insect damage.Journal of Economic Entomology, 4, 1382-1390

Sachs E S, Benedict J H, Stelly D M, Taylor J F, Altman D W,Berberich S A, Davis S K. 1998. Expression and segregationof genes encoding CryIAc insecticidal proteins in cotton.Crop Science, 38, 1-11

Shen P, Lin K J, Zhang Y J, Wu K M, Guo Y Y. 2010. Seasonalexpression of Bacillius thuingiensis insecticidal protein andcontrol to cotton bollworm in different varieties of transgeniccotton. Cotton Science, 22, 393-397 (in Chinese)

Tonhazy N E, White N G, Umbriet W W. 1950. Colorimetricassay of Glutamic-pyruvic transaminase. Arch BiochemBiophys, 28, 36-38

Vance C P, Heichel G H, Barnes D K, Bryan J M, Johnson L E.1979. Nitrogen fixation, nodule development, and vegetativeregrowth of alfalfa (Medicago sativa L.) following harvest.Plant Physiology, 64, 1-8

Vermon A W. 1979. Ribulosebisphosphate carboxylase andprotealytic activity in wheat leaves from anthesis throughsenescence. Plant Physiology, 64, 884-887

Wang B G, Chen Y T. 2012. The occurrence characteristicsand countermeasure of prevention and cure for cotton bollworm in Wuwei County. Anhui Agricultural Science Bulletin,18, 115-116 (in Chinese)

Wang L M, Wang J B, Sheng F F, Zhang X K, Liu R Z. 2001.Influences of waterlogging and drought on differenttransgenic Bt cotton cultivars. Cotton Science, 2, 87-90(in Chinese)

Weiler E W, Jourdan P S, Conrad W. 1981. Levels of indole-3-aceticacid and intact decapitated coleoptiples as determinedby a specific and highly sensitive solid-phase enzymeimmuno-assay. Planta, 153, 561-571

Xia L Q, Guo S D. 2004. High temperature on Bt geneexpression of Bt cotton. Scientia Agricultura Sinica, 37,1733-1737 (in Chinese)

Zhang X, Zhang L, Ye G Y, Wang Y H, Chen Y, Chen D H.2007. The impact of introducing the Bacillus Thuringiensisgene into cotton on boll nitrogen metabolism. Environmentaland Experimental Botany, 61, 175-180

Yemm E W, Cocking E C. 1955. Determination of amino acidwith ninhydrin. Analyst, 80, 209-213

Zhao J Z, Zhou C H, Lu M G, Fan X L, Rong L J, Meng X Q. 2000. Monitoring and management of Helicoverpaarmigeraresistance to transgenic Bt cotton in Northern China.Resistant Pest Management, 1, 28-31 (in Chinese)

Zhen L, Dong H Z, Li W J, Zhao M, Zhu Y Q. 2008. Individualand combined effects of salinity and waterlogging on Cry1Acprotein expression and insecticidal efficacy of Bt cotton.Crop Protection, 27, 1485-1490

Zhou C K. 1999. The characteristics and trend analyze ofair temperature in both winter and summer for past halfcentury in Nanjing region. Scientia Meteorologica Sinica,3, 310-319 (in Chinese)

Zhou D S, Wu Z T. 2000. Effect of temperature and nitrogenfertilizer on insect resistance of Bt cotton. Journal of AnhuiAgriculture University, 4, 352-357 (in Chinese)

Zhou G Y, Yang F X, Fu X Q, Wang X L. 1996. The influenceof climatic differences in the Yangtze River Valley oncotton yield and fiber quality. China Cottons, 10, 15-18(in Chinese)
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