Please wait a minute...
Journal of Integrative Agriculture  2026, Vol. 25 Issue (8): 3153-3168    DOI: 10.1016/j.jia.2024.12.016
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Two genes of cytochrome P450 regulate plant height via brassinosteroid biosynthesis in Brassica napus

Qianqian Zheng1*, Xinhua Wang1*, Zhenzhen Wang1, Yi Zhang1, Hao Wang3, Kangxi Du3, Shaohong Fu2, Wanzhuo Gong2, Hua Yuan3, Weilan Chen3, Bin Tu3, Jin Yang2#, Yun Li2#, Ting Li1#

1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Sichuan Agricultural University, Chengdu 611130, China

2 Chengdu Research Branch, Chengdu Academy of Agriculture and Forestry Sciences/National Rapeseed Genetic Improvement Center, Chengdu 611130, China

3 Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China

 Highlights 
This study identified two cytochrome P450 genes, BnaA10.CYP90A1 and BnaC09.CYP90A1, as key regulators of plant height in Brassica napus, highlighting their roles in brassinosteroid biosynthesis and plant growth modulation.
Mutations in BnaA10.CYP90A1 and BnaC09.CYP90A1 genes lead to reduced levels of bioactive brassinosteroids, significantly impairing cell elongation and causing a dwarf phenotype in rapeseed.
The single mutation of BnaA10.CYP90A1 produced semi-dwarf plants, suggesting its potential for developing new rapeseed cultivars with optimized plant height for enhanced yield and mechanical harvesting efficiency.
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

油菜,作为全球重要的油料作物之一,是食用植物油的重要来源。现有研究表明优化油菜株型是提高油菜单位面积产量的关键因素之一,但其遗传基础和分子调控机制研究尚未明确。为了创制油菜矮杆新种质挖掘控制株高的新基因,我们利用EMS诱变技术对油菜品种中双11”进行诱变筛选,获得一份矮杆突变体dm1。形态学分析显示发现dm1矮化表型是由于细胞长度显著减少导致的。结合BSA-seq进行定位分析,鉴定参与dm1株高调控的基因是BnaA10.CYP90A1BnaC09.CYP90A1。上述2个基因编码细胞色素酶P450,与拟南芥中的AtCPD/AtCYP90A1同源。AtCPD/AtCYP90A1对油菜素内酯(BR)的生物合成至关重要,在本文研究中,我们也发现dm1中具有生物活性油菜素内酯(CS)及其前体6-脱氧CS水平显著降低,导致下游参与细胞扩张相关基因表达下调。此外,BR水平的降低还通过负反馈机制促进了BR生物合成基因的表达。最后我们分离获得BnaA10.CYP90A1的单突突变体,发现BnaA10.CYP90A1基因的单一突变导致了植株呈现出半矮化表型,这种表型有望应用于未来的半矮杆育种,以实现产量和机械收获的平衡。综上所述,以上研究表明BnaCYP90A1s参与油菜中油菜素内酯生物合成,并证实它们在调控油菜植株高度中的重要作用,为油菜株型改良提供了新的基因资源和遗传材料



Abstract  

Rapeseed (Brassica napus) is one of the most important oil crops worldwide and provides a major source of edible vegetable oil.  Currently, manipulating plant height with branching effectively balances biomass and yields.  However, the genetic mechanisms to control plant height remain largely unknown in rapeseed.  To address this gap, we isolated an extremely dwarf mutant (dm1) from ethyl-methanesulfonate (EMS) mutagenesis and revealed that the dwarfism results from a significant reduction in cell length.  Bulk segregant analysis (BSA) identified BnaA10.CYP90A1 and BnaC09.CYP90A1 as the causative genes of dm1.  Both genes encoded the proteins homologous to the Arabidopsis cytochrome P450 AtCPD/AtCYP90A1, which is crucial for brassinosteroid (BR) biosynthesis.  In this regard, we demonstrated reduced levels of bioactive BRs, castasterone (CS), and its precursor 6-deoxoCS in dm1, resulting in down-regulation of various genes involved in cell expansion.  The reduced BR levels also caused negative feedback, promoting the expression of BR biosynthetic genes in dm1.  Furthermore, we proved that the single mutation of BnaA10.CYP90A1 gene conferred semi-dwarfism, potentially beneficial for producing an ideal type of plant to improve cultivars with a balance of yield and machinery harvest through genetic modifications.  Collectively, these findings highlighted the critical role of BnaCYP90A1s in BR biosynthesis and validated their influence on plant height regulation in rapeseed.

Keywords:  plant height       BR biosynthesis        cytochrome P450        Brassica napus   
Received: 09 August 2024   Accepted: 21 November 2024 Online: 13 December 2024  
Fund: 

This work was supported by the Science and Technology Innovation 2030-Major Project, China (2023ZD04069), the Natural Science Foundation of Sichuan Province, China (23NSFSC0765), the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation (GZB20230498), the Key Research and Development Projects of Chengdu, China (2023-YF09-00024-SN), and the Sichuan Innovation Team Project, China (SCCXTD-2024-3).

About author:  #Correspondence Ting Li, Tel: +86-28-86293167, E-mail: tinli@sicau.edu.cn; Yun Li, Tel: +86-28-82746572, E-mail: liyunxpzh@163.com; Jin Yang, Tel: +86-28-82746572, E-mail: yjjing@163.com * These authors contributed equally to this study.

Cite this article: 

Qianqian Zheng, Xinhua Wang, Zhenzhen Wang, Yi Zhang, Hao Wang, Kangxi Du, Shaohong Fu, Wanzhuo Gong, Hua Yuan, Weilan Chen, Bin Tu, Jin Yang, Yun Li, Ting Li. 2026. Two genes of cytochrome P450 regulate plant height via brassinosteroid biosynthesis in Brassica napus. Journal of Integrative Agriculture, 25(8): 3153-3168.

Achard P, Gusti A, Cheminant S, Alioua M, Dhondt S, Coppens F, Beemster G T, Genschik P. 2009. Gibberellin signaling controls cell proliferation rate in ArabidopsisCurrent Biology19, 1188–1193.

Bancoş S, Nomura T, Sato T, Molnár G, Bishop G J, Koncz C, Yokota T, Nagy F, Szekeres M. 2002. Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis. Plant Physiology130, 504–513.

Bancos S, Szatmári A M, Castle J, Kozma-Bognár L, Shibata K, Yokota T, Bishop G J, Nagy F, Szekeres M. 2006. Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in ArabidopsisPlant Physiology141, 299–309.

Bellido A M, Distéfano A M, Setzes N, Cascallares M M, Oklestkova J, Novak O, Ramirez J A, Zabaleta E J, Fiol D F, Pagnussat G C. 2022. A mitochondrial ADXR-ADX-P450 electron transport chain is essential for maternal gametophytic control of embryogenesis in ArabidopsisProceedings of the National Academy of Sciences of the United States of America119, e2000482119.

Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant13, 1194–1202.

Chen L, Duan L, Sun M, Yang Z, Li H, Hu K, Yang H, Liu L. 2022. Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development. Frontiers in Plant Science13, 1052569.

Chen X, Hu X, Jiang J, Wang X. 2024. Functions and mechanisms of brassinosteroids in regulating crop agronomic traits. Plant and Cell Physiology65, 1568–1580.

Cheng H, Jin F, Zaman Q U, Ding B, Hao M, Wang Y, Huang Y, Wells R, Dong Y, Hu Q. 2019. Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape. BMC Plant Biology19, 500.

Cosgrove D J. 2024. Structure and growth of plant cell walls. Nature Reviews Molecular Cell Biology25, 340–358.

Du X, Hussain N, Li Z, Chen X, Hua S, Zhang D, Jiang L. 2015. Effect of gibberellin on the biosynthesis of tocopherols in oilseed rape (Brassica napus L.) and ArabidopsisJournal of Agricultural and Food Chemistry63, 360–369.

Gan Q, Luan M, Hu M, Liu Z, Zhang Z. 2022. Functional study of CYP90A1 and ALDH3F1 gene obtained by transcriptome sequencing analysis of Brassica napus seedlings treated with brassinolide. Frontiers in Plant Science13, 1040511.

Gao J, Qin P, Tang S, Guo L, Dai C, Wen J, Yi B, Ma C, Shen J, Fu T, Zou J, Tu J. 2024. A gain-of-function mutation in BnaIAA13 disrupts vascular tissue and lateral root development in Brassica napusJournal of Experimental Botany75, 5592–5610.

Gudesblat G E, Russinova E. 2011. Plants grow on brassinosteroids. Current Opinion in Plant Biology14, 530–537.

Guo R, Qian H, Shen W, Liu L, Zhang M, Cai C, Zhao Y, Qiao J, Wang Q. 2013. BZR1 and BES1 participate in regulation of glucosinolate biosynthesis by brassinosteroids in ArabidopsisJournal of Experimental Botany64, 2401–2412.

Hong Z, Ueguchi-Tanaka M, Fujioka S, Takatsuto S, Yoshida S, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M. 2005. The rice brassinosteroid-deficient dwarf2 mutant, defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1, is rescued by the endogenously accumulated alternative bioactive brassinosteroid, dolichosterone. The Plant Cell17, 2243–2254.

Hu D, Jing J, Snowdon R J, Mason A S, Shen J, Meng J, Zou J. 2021. Exploring the gene pool of Brassica napus by genomics-based approaches. Plant Biotechnology Journal19, 1693–1712.

Hu Q, Hua W, Yin Y, Zhang X, Liu L, Shi J, Zhao Y, Qin L, Chen C, Wang H. 2017. Rapeseed research and production in China. The Crop Journal5, 127–135.

Kim E J, Russinova E. 2020. Brassinosteroid signalling. Current Biology30, R294–R298.

Kim G T, Tsukaya H, Uchimiya H. 1998. The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P-450 family that is required for the regulated polar elongation of leaf cells. Genes & Development12, 2381–2391.

Kim H B, Kwon M, Ryu H, Fujioka S, Takatsuto S, Yoshida S, An C S, Lee I, Hwang I, Choe S. 2006. The regulation of DWARF4 expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in ArabidopsisPlant Physiology140, 548–557.

Kim T W, Hwang J Y, Kim Y S, Joo S H, Chang S C, Lee J S, Takatsuto S, Kim S K. 2005. Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis. The Plant Cell17, 2397–2412.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution33, 1870–1874.

Li B, Liu X, Guo Y, Deng L, Qu L, Yan M, Li M, Wang T. 2023. BnaC01.BIN2, a GSK3-like kinase, modulates plant height and yield potential in Brassica napusTheoretical and Applied Genetics136, 29.

Li H, Li J, Song J, Zhao B, Guo C, Wang B, Zhang Q, Wang J, King G J, Liu K. 2019. An auxin signaling gene BnaA3.IAA7 contributes to improved plant architecture and yield heterosis in rapeseed. New Phytologist222, 837–851.

Li T, Wang Y, Natran A, Zhang Y, Wang H, Du K, Qin P, Yuan H, Chen W, Tu B, Inzé D, Dubois M. 2024. C-TERMINAL DOMAIN PHOSPHATASE-LIKE 3 contributes to GA-mediated growth and flowering by interaction with DELLA proteins. New Phytologist242, 2555–2569.

Liu C, Wang J, Huang T, Wang F, Yuan F, Cheng X, Zhang Y, Shi S, Wu J, Liu K. 2010. A missense mutation in the VHYNP motif of a DELLA protein causes a semi-dwarf mutant phenotype in Brassica napusTheoretical and Applied Genetics121, 249–258.

Liu S, Raman H, Xiang Y, Zhao C, Huang J, Zhang Y. 2022. De novo design of future rapeseed crops: Challenges and opportunities. The Crop Journal10, 587–596.

Marowa P, Ding A, Kong Y. 2016. Expansins: Roles in plant growth and potential applications in crop improvement. Plant Cell Reports35, 949–965.

Noguchi T, Fujioka S, Takatsuto S, Sakurai A, Yoshida S, Li J, Chory J. 1999. Arabidopsis det2 is defective in the conversion of (24R)–24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis. Plant Physiology120, 833–840.

Ohnishi T, Godza B, Watanabe B, Fujioka S, Hategan L, Ide K, Shibata K, Yokota T, Szekeres M, Mizutani M. 2012. CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation. Journal of Biological Chemistry287, 31551–31560.

Pham V N, Kathare P K, Huq E. 2018. Dynamic regulation of PIF5 by COP1-SPA complex to optimize photomorphogenesis in ArabidopsisThe Plant Journal96, 260–273.

Ping X, Ye Q, Yan M, Zeng J, Yan X, Li H, Li J, Liu L. 2022. Integrated genetic
mapping and transcriptome analysis reveal the BnaA03.IAA7 protein regulates plant architecture and gibberellin signaling in Brassica napus L. Theoretical and Applied Genetics135, 3497–3510.

Robert X, Gouet P. 2014. Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Research42, W320–W324.

Shi L, Song J, Guo C, Wang B, Guan Z, Yang P, Chen X, Zhang Q, King G J, Wang J, Liu K. 2019. A CACTA-like transposable element in the upstream region of BnaA9.CYP78A9 acts as an enhancer to increase silique length and seed weight in rapeseed. The Plant Journal98, 524–539.

Song L, Liu J, Cao B, Liu B, Zhang X, Chen Z, Dong C, Liu X, Zhang Z, Wang W, Chai L, Liu J, Zhu J, Cui S, He F, Peng H, Hu Z, Su Z, Guo W, Xin M, et al. 2023. Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat. Nature617, 118–124.

Song M, Linghu B, Huang S, Li F, An R, Xie C, Zhu Y, Hu S, Mu J, Zhang Y. 2022. Genome-wide survey of leucine-rich repeat receptor-like protein kinase genes and CRISPR/Cas9-targeted mutagenesis BnBRI1 in Brassica napusFrontiers in Plant Science13, 865132.

Sugihara Y, Young L, Yaegashi H, Natsume S, Shea D J, Takagi H, Booker H, Innan H, Terauchi R, Abe A. 2022. High-performance pipeline for MutMap and QTL-seq. PeerJ10, e13170.

Sun C, Wang B, Yan L, Hu K, Liu S, Zhou Y, Guan C, Zhang Z, Li J, Zhang J, Chen S, Wen J, Ma C, Tu J, Shen J, Fu T, Yi B. 2016. Genome-wide association study provides insight into the genetic control of plant height in rapeseed (Brassica napus L.). Frontiers in Plant Science7, 1102.

Tong J, Zhao W, Wang K, Deng D, Xiao L. 2024. Organ-level distribution tandem mass spectrometry analysis of three structural types of brassinosteroids in rapeseed. Frontiers in Plant Science15, 1308781.

Vayghan H S, Nawrocki W J, Schiphorst C, Tolleter D, Hu C, Douet V, Glauser G, Finazzi G, Croce R, Wientjes E, Longoni F. 2022. Photosynthetic light harvesting and thylakoid organization in a CRISPR/Cas9 Arabidopsis thaliana LHCB1 knockout mutant. Frontiers in Plant Science13, 833032.

Wan S, Wu J, Zhang Z, Sun X, Lv Y, Gao C, Ning Y, Ma J, Guo Y, Zhang Q, Zheng X, Zhang C, Ma Z, Lu T. 2009. Activation tagging, an efficient tool for functional analysis of the rice genome. Plant Molecular Biology69, 69–80.

Wang X, Pan C, Long J, Bai S, Yao M, Chen J, Sun G, Fan Y, Wang Z, Liu F, Liu C, Li Q. 2022. Genome-wide identification of the jumonji C domain- containing histone demethylase gene family in wheat and their expression analysis under drought stress. Frontiers in Plant Science13, 987257.

Wang Y, Perez-Sancho J, Platre M P, Callebaut B, Smokvarska M, Ferrer K, Luo Y, Nolan T M, Sato T, Busch W, Benfey P N, Kvasnica M, Winne J M, Bayer E M, Vukašinović N, Russinova E. 2023. Plasmodesmata mediate cell-to-cell transport of brassinosteroid hormones. Nature Chemical Biology19, 1331–1341.

Wei T, Zhang L, Zhu R, Jiang X, Yue C, Su Y, Ren H, Wang M. 2021. A gain-of-function mutant of IAA7 inhibits stem elongation by transcriptional repression of EXPA5 genes in Brassica napusInternational Journal of Molecular Sciences22, 9018.

Wei Z, Li J. 2020. Regulation of brassinosteroid homeostasis in higher plants. Frontiers in Plant Science11, 583622.

Wolf S. 2022. Cell wall signaling in plant development and defense. Annual Review of Plant Biology73, 323–353.

Wu M, Ren Y, Cai M, Wang Y, Zhu S, Zhu J, Hao Y, Teng X, Zhu X, Jing R, Zhang H, Zhong M, Wang Y, Lei C, Zhang X, Guo X, Cheng Z, Lin Q, Wang J, Jiang L, et al. 2019. Rice FLOURY ENDOSPERM10 encodes a pentatricopeptide repeat protein that is essential for the trans-splicing of mitochondrial nad1 intron 1 and endosperm development. New Phytologist223, 736–750.

Yang M, He J, Wan S, Li W, Chen W, Wang Y, Jiang X, Cheng P, Chu P, Shen W, Guan R. 2021. Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L. BMC Plant Biology21, 359.

Yang Z, Wang S, Wei L, Huang Y, Liu D, Jia Y, Luo C, Lin Y, Liang C, Hu Y, Dai C, Guo L, Zhou Y, Yang Q Y. 2023. BnIR: A multi-omics database with various tools for Brassica napus research and breeding. Molecular Plant16, 775–789.

Zebosi B, Vollbrecht E, Best N B. 2024. Brassinosteroid biosynthesis and signaling: conserved and diversified functions of core genes across multiple plant species. Plant Communications5, 100982.

Zhan H, Lu M, Luo Q, Tan F, Zhao Z, Liu M, He Y. 2022. OsCPD1 and OsCPD2 are functional brassinosteroid biosynthesis genes in rice. Plant Science325, 111482.

Zhang H, Zhao D, Tang Z, Zhang Y, Zhang K, Dong J, Wang F. 2022. Exogenous brassinosteroids promotes root growth, enhances stress tolerance, and increases yield in maize. Plant Signaling & Behavior17, 2095139.

Zhang X, Lin R. 2017. Light signaling differentially regulates the expression of group IV of the B-box zinc finger family. Plant Signaling & Behavior12, e1365213.

Zhao B, Li H, Li J, Wang B, Dai C, Wang J, Liu K. 2017. Brassica napus DS-3, encoding a DELLA protein, negatively regulates stem elongation through gibberellin signaling pathway. Theoretical and Applied Genetics130, 727–741.

Zhao B, Li J. 2012. Regulation of brassinosteroid biosynthesis and inactivation. Journal of Integrative Plant Biology54, 746–759.

Zhao C, Yang L, Tang M, Liu L, Huang J, Tong C, Xiang Y, Liu S, Cheng X, Xie M. 2022. Genome-wide association study reveals a GLYCOGEN SYNTHASE KINASE 3 gene regulating plant height in Brassica napusFrontiers in Plant Science13, 1061196.

Zheng M, Terzaghi W, Wang H, Hua W. 2022. Integrated strategies for increasing rapeseed yield. Trends in Plant Science27, 742–745.

Zheng M, Zhang L, Tang M, Liu J, Liu H, Yang H, Fan S, Terzaghi W, Wang H, Hua W. 2020. Knockout of two BnaMAX1 homologs by CRISPR/Cas9-targeted mutagenesis improves plant architecture and increases yield in rapeseed (Brassica napus L.). Plant Biotechnology Journal18, 644–654.

Zhiponova M K, Vanhoutte I, Boudolf V, Betti C, Dhondt S, Coppens F, Mylle E, Maes S, González-García M P, Caño-Delgado A I, Inzé D, Beemster G T S, De Veylder L, Russinova E. 2013. Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. New Phytologist197, 490–502.

Zhou X, Zhang H, Wang P, Liu Y, Zhang X, Song Y, Wang Z, Ali A, Wan L, Yang G, Hong D. 2022. BnaC7.ROT3, the causal gene of cqSL-C7, mediates silique length by affecting cell elongation in Brassica napusJournal of Experimental Botany73, 154–167.

Zhu L, Wang H, Zhu J, Wang X, Jiang B, Hou L, Xiao G. 2023. A conserved brassinosteroid-mediated BES1-CERP-EXPA3 signaling cascade controls plant cell elongation. Cell Reports42, 112301.


[1] Zhiying Zhao, Wanting Li, Yifei Wang, Meng Jin, Wenqiang Tang, Jiayi Li, Renliang Zhang, Yaxian Zhang, Peiyong Xin, Jinfang Chu, Yingjie Gao, Sha Tang, Xianmin Diao, Baowen Zhang. Proteomic investigation reveals the molecular mechanisms of plant height regulation in foxtail millet[J]. >Journal of Integrative Agriculture, 2026, 25(4): 1402-1417.
[2] Shengzhong Zhang, Xiaohui Hu, Feifei Wang, Huarong Miao, Chu Ye, Weiqiang Yang, Wen Zhong, Jing Chen. Identification of QTLs for plant height and branching-related traits in cultivated peanut[J]. >Journal of Integrative Agriculture, 2025, 24(7): 2511-2524.
[3] Shuliang Jiao, Qinyan Li, Fan Zhang, Yonghong Tao, Yingzhen Yu, Fan Yao, Qingmao Li, Fengyi Hu, Liyu Huang.

Artificial selection of the Green Revolution gene Semidwarf 1 is implicated in upland rice breeding [J]. >Journal of Integrative Agriculture, 2024, 23(3): 769-780.

[4] LIU Yan, WANG Wei-ping, ZHANG Lin, ZHU Long-fu, ZHANG Xian-long, HE Xin. The HD-Zip transcription factor GhHB12 represses plant height by regulating the auxin signaling in cotton[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2015-2024.
[5] WANG Xiao-dong, CAI Ying, PANG Cheng-ke, ZHAO Xiao-zhen, SHI Rui, LIU Hong-fang, CHEN Feng, ZHANG Wei, FU San-xiong, HU Mao-long, HUA Wei, ZHENG Ming, ZHANG Jie-fu. BnaSD.C3 is a novel major quantitative trait locus affecting semi-dwarf architecture in Brassica napus L.[J]. >Journal of Integrative Agriculture, 2023, 22(10): 2981-2992.
[6] LIU Chen, TIAN Yu, LIU Zhang-xiong, GU Yong-zhe, ZHANG Bo, LI Ying-hui, NA Jie, QIU Li-juan. Identification and characterization of long-InDels through whole genome resequencing to facilitate fine-mapping of a QTL for plant height in soybean (Glycine max L. Merr.)[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1903-1912.
[7] TIAN Yu, YANG Lei, LU Hong-feng, ZHANG Bo, LI Yan-fei, LIU Chen, GE Tian-li, LIU Yu-lin, HAN Jia-nan, LI Ying-hui, QIU Li-juan. QTL analysis for plant height and fine mapping of two environmentally stable QTLs with major effects in soybean[J]. >Journal of Integrative Agriculture, 2022, 21(4): 933-946.
[8] WANG Xin-yu, XU Le, LI Xiao-xiao, YANG Guo-dong, WANG Fei, PENG Shao-bing. Grain yield and lodging-related traits of ultrashort-duration varieties for direct-seeded and double-season rice in Central China[J]. >Journal of Integrative Agriculture, 2022, 21(10): 2888-2899.
[9] LI Jie-ping, Soomro Ayaz Ali, XIAO Gui, CHEN Fan-jun, YUAN Li-xing, GU Ri-liang. Phenotypic characterization and genetic mapping of the dwarf mutant m34 in maize[J]. >Journal of Integrative Agriculture, 2019, 18(5): 948-957.
[10] WANG Yi-xue, XU Qiao-fang, CHANG Xiao-ping, HAO Chen-yang, LI Run-zhi, JING Rui-lian. A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat[J]. >Journal of Integrative Agriculture, 2018, 17(2): 276-284.
[11] WANG Jiang-xu, SUN Jian, LI Cheng-xin, LIU Hua-long, WANG Jing-guo, ZHAO Hong-wei, ZOU De-tang. Genetic dissection of the developmental behavior of plant height in rice under different water supply conditions[J]. >Journal of Integrative Agriculture, 2016, 15(12): 2688-2702.
[12] WANG Hong-qiu, ZHANG Xiang-ge, YANG Hui-li, CHEN Yong-qiang, YUAN Liang, LI Wei-hua, LIU Zong-hua, TANG Ji-hua, KANG Ding-ming. Heterotic loci identified for plant height and ear height using two CSSLs test populations in maize[J]. >Journal of Integrative Agriculture, 2016, 15(12): 2726-2735.
[13] LI Qian, WANG Jing-yi, Nadia Khan, CHANG Xiao-ping, LIU Hui-min, JING Rui-lian. Polymorphism and association analysis of a drought-resistant gene TaLTP-s in wheat[J]. >Journal of Integrative Agriculture, 2016, 15(06): 1198-1206.
No Suggested Reading articles found!