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Journal of Integrative Agriculture  2016, Vol. 15 Issue (06): 1198-1206    DOI: 10.1016/S2095-3119(15)61189-3
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Polymorphism and association analysis of a drought-resistant gene TaLTP-s in wheat
LI Qian1, 2*, WANG Jing-yi1*, Nadia Khan1, CHANG Xiao-ping1, LIU Hui-min2, JING Rui-lian1
1 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 College of Bioengineering, Shanxi University, Taiyuan 030006, P.R.China
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Abstract     Lipid transfer protein (LTP) is a kind of small molecular protein, which is named for its ability to transfer lipid between cell membranes. It has been proved that the protein is involved in the responding to abiotic stresses. In this study, TaLTP-s, a genomic sequence of TaLTP was isolated from A genome of wheat (Triticum aestivum L). Sequencing analysis exhibited that there was no diversity in the coding region of TaLTP-s, but seven single nucleotide polymorphisms (SNPs) and 1 bp insertion/deletion (InDel) were detected in the promoter regions of different wheat accessions. Nucleotide diversity (π) in the region was 0.00033, and linkage disequilibrium (LD) extended over almost the entire TaLTP-s region in wheat. The dCAPS markers based on sequence variations in the promoter regions (SNP-207 and SNP-1696) were developed, and three haplotypes were identified based on those markers. Association analysis between the haplotypes and agronomic traits of natural population consisted of 262 accessions showed that three haplotypes of TaLTP-s were significantly associated with plant height (PH). Among the three haplotypes, HapIII is considered as the superior haplotype for increasing plant height in the drought stress environments. The G variance at the position of 207 bp could be a superior allele that significantly increased number of spikes per plant (NSP). The functional marker of TaLTP-s provide a tool for marker-assisted selection regarding to plant height and number of spikelet per plant in wheat.
Keywords:  Triticum aestivum L.        TaLTP        single nucleotide polymorphism        association analysis        plant height  
Received: 04 May 2015   Accepted:

This study was supported by the National High-Tech R&D Program of China (2011AA100501), the National Natural Science Foundation of China (31461143024) and the Agricultural Science and Technology Innovation Program (ASTIP), Chinese Academy of Agricultural Sciences.

Corresponding Authors:  JING Rui-lian, Tel/Fax: +86-10-82105829, E-mail:   

Cite this article: 

LI Qian, WANG Jing-yi, Nadia Khan, CHANG Xiao-ping, LIU Hui-min, JING Rui-lian. 2016. Polymorphism and association analysis of a drought-resistant gene TaLTP-s in wheat. Journal of Integrative Agriculture, 15(06): 1198-1206.

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