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| Polymorphism and association analysis of a drought-resistant gene TaLTP-s in wheat |
| LI Qian1, 2*, WANG Jing-yi1*, Nadia Khan1, CHANG Xiao-ping1, LIU Hui-min2, JING Rui-lian1 |
1 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 College of Bioengineering, Shanxi University, Taiyuan 030006, P.R.China |
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Abstract Lipid transfer protein (LTP) is a kind of small molecular protein, which is named for its ability to transfer lipid between cell membranes. It has been proved that the protein is involved in the responding to abiotic stresses. In this study, TaLTP-s, a genomic sequence of TaLTP was isolated from A genome of wheat (Triticum aestivum L). Sequencing analysis exhibited that there was no diversity in the coding region of TaLTP-s, but seven single nucleotide polymorphisms (SNPs) and 1 bp insertion/deletion (InDel) were detected in the promoter regions of different wheat accessions. Nucleotide diversity (π) in the region was 0.00033, and linkage disequilibrium (LD) extended over almost the entire TaLTP-s region in wheat. The dCAPS markers based on sequence variations in the promoter regions (SNP-207 and SNP-1696) were developed, and three haplotypes were identified based on those markers. Association analysis between the haplotypes and agronomic traits of natural population consisted of 262 accessions showed that three haplotypes of TaLTP-s were significantly associated with plant height (PH). Among the three haplotypes, HapIII is considered as the superior haplotype for increasing plant height in the drought stress environments. The G variance at the position of 207 bp could be a superior allele that significantly increased number of spikes per plant (NSP). The functional marker of TaLTP-s provide a tool for marker-assisted selection regarding to plant height and number of spikelet per plant in wheat.
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Received: 04 May 2015
Accepted:
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| Fund: This study was supported by the National High-Tech R&D Program of China (2011AA100501), the National Natural Science Foundation of China (31461143024) and the Agricultural Science and Technology Innovation Program (ASTIP), Chinese Academy of Agricultural Sciences. |
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Corresponding Authors:
JING Rui-lian, Tel/Fax: +86-10-82105829, E-mail: jingruilian@caas.cn
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Cite this article:
LI Qian, WANG Jing-yi, Nadia Khan, CHANG Xiao-ping, LIU Hui-min, JING Rui-lian.
2016.
Polymorphism and association analysis of a drought-resistant gene TaLTP-s in wheat. Journal of Integrative Agriculture, 15(06): 1198-1206.
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| Agrama H, Eizenga G, Yan W. 2007. Association mapping of yield and its components in rice cultivars. Molecular Breeding, 19, 341–356.Bradbury P J, Zhang Z, Kroon D E, Casstevens T M, Ramdoss Y, Buckler E S. 2007. TASSEL: Software for association mapping of complex traits in diverse samples. Bioinformatics, 23, 2633–2635.Cameron K D, Teece M A, Smart L B. 2006. Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiology, 140, 176–183.Debono A, Yeats T H, Rose J K, Bird D, Jetter R, Kunst L, Samuels L. 2009. Arabidopsis LTPG is a glycosylphosphatidylinositol-anchored lipid transfer protein required for export of lipids to the plant surface. The Plant Cell, 21, 1230–1238.Garces-Claver A, Fellman S M, Gil-Ortega R, Jahn M, Arnedo-Andres M S. 2007. Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp. Theoretical and Applied Genetics, 115, 907–916.Guo C, Ge X, Ma H. 2013. The rice OsDIL gene plays a role in drought tolerance at vegetative and reproductive stages. Plant Molecular Biology, 82, 239–253.Gupta P K, Rustgi S, Kulwal P L. 2005. Linkage disequilibrium and association studies in higher plants: Present status and future prospects. Plant Molecular Biology, 57, 461–485.Hill W G, Robertson A. 1968. Linkage disequilibrium in finite populations. Theoretical and Applied Genetics, 38, 226–231.Jose E M, Gomis R F X, Puigdomenech P. 2004. The eight-cysteine motif, a versatile structure in plant proteins. Plant Physiology and Biochemistry, 42, 355–365.Kader J C. 1996. Lipid-transfer proteins in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 47, 627–654.Li P F, Cheng Z G, Zhao H, Zhang X F, Li J N, Wang S M, Xiong Y C. 2011. Currentprogress in plant ideotype research of dryland wheat (Triticum aestivum L.). Acta Ecologica Sinica, 31, 2631–2640. (in Chinese)Li Q, Wang J Y, MAO X G, Li A, Gao L F, Liu H M, Jing R L. 2015. Cloning and functional analysis of lipid transfer protein gene TaLTP in wheat. Acta Agronomica Sinica, 41, 673–682. (in Chinese)Li W Y, Zhang B, Zhang J N, Chang X P, Li R Z, Jing R L. 2012. Exploring elite alleles for chlorophyll content of flag leaf in natural population of wheat by association analysis. Acta Agronomica Sinica, 38, 962–970. (in Chinese)Lu Y L, Xu J, Yuan Z, Hao Z, Xie C, Li X, Shah T, Lan H S, Zhang S, Rong T. 2011. Comparative LD mapping using single SNPs and haplotypes identifies QTL for plant height and biomass as secondary traits of drought tolerance in maize. Molecular Breeding, 30, 407–418.Maldonado A M, Doerner P, Dixon R A, Lamb C J, Cameron R K. 2002. A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis. Nature, 419, 399–403.Maldonado-Calderón M, Sepúlveda-García E, Rocha-Sosa M. 2012. Characterization of novel F-box proteins in plants induced by biotic and abiotic stress. Plant Science, 185–186, 208–217.Mir R, Kumar N, Jaiswal V, Girdharwal N, Prasad M, Balyan H, Gupta P. 2012. Genetic dissection of grain weight in bread wheat through quantitative trait locus interval and association mapping. Molecular Breeding, 29, 1–10.Nasu S, Suzuki J, Ohta R, Hasegawa K, Yui R, Kitazawa N, Monna L, Minobe Y. 2002. Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers. DNA Research, 9, 163–171.Pitzschke A, Datta S, Persak H. 2014. Salt stress in Arabidopsis: lipid transfer protein AZI1 and its control by mitogen-activated protein kinase MPK3. Molecular Plant, 7, 722–738.Sari-Gorla M, Krajewski P, Di Fonzo N, Villa M, Frova C. 1999. Genetic analysis of drought tolerance in maize by molecular markers. II. Plant height and flowering. Theoretical and Applied Genetics, 99, 289–295.Su J Y, Zheng Q, Li H W, Li B, Jing R L, Tong Y P, Li Z S. 2009. Detection of QTLs for phosphorus use efficiency in relation to agronomic performance of wheat grown under phosphorus sufficient and limited conditions. Plant Science, 176, 824–836.Thornsberry J M, Goodman M M, Doebley J, Kresovich S, Nielsen D, Buckler E S. 2001. Dwarf8 polymorphisms associate with variation in flowering time. Nature Genetics, 28, 286–289.Wang Q, Mao X G, Chang X P, Jia J Z, Liu H M, Jing R L. 2014. Polymorphism of TaSnRK2.10 and its association with yield-related traits in wheat. Scientia Agricultura Sinica, 47, 1865–1877. (in Chinese)Wang Y M, Zhang Z B, Liu C M, Zhong G C, Xu P. 2004. The production status of drought-resisting and water-saving wheat varieties and the breeding countermeasures in Hebei province. Chinese Journal of Eco-Agriculture, 12, 142–144.(in Chinese)Wang Z H, Wu X S, Ren Q, Chang X P, Li R Z, Jing R L. 2010. QTL mapping for developmental behavior of plant height in wheat (Triticum aestivum L.). Euphytica, 174, 447–458.Wu G, Robertson A J, Liu X, Zheng P, Wilen R W, Nesbitt N T, Gusta L V. 2004. A lipid transfer protein gene BG-14 is differentially regulated by abiotic stress, ABA, anisomycin, and sphingosine in bromegrass (Bromus inermis). Journal of Plant Physiology, 161, 449–458.Wu X S, Chang X P, Jing R L. 2012. Genetic insight into yield-associated traits of wheat grown in multiple rain-fed environments. PLoS ONE, 7, e31249.Wu X S, Wang Z H, Chang X P, Jing R L. 2010. Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes. Journal of Experimental Botany, 61, 2923–2937.Yang D L, Jing R L, Chang X P, Li W. 2007. Identification of quantitative trait loci and environmental interactions for accumulation and remobilization of water-soluble carbohydrates in wheat (Triticum aestivum L.) stems. Genetics, 176, 571–584.Zhang B, Shi W, Li W Y, Chang X P, Jing R L. 2013. Efficacy of pyramiding elite alleles for dynamic development of plant height in common wheat. Molecular Breeding, 32, 327–338.Zhang J N, Hao C Y, Ren Q, Chang X P, Liu G R , Jing R L. 2011. Association mapping of dynamic developmental plant height in common wheat. Planta, 234, 891–902.Zheng J, Liu H, Wang Y, Wang L, Chang X, Jing R, Hao C, Zhang X. 2014. TEF-7A, a transcript elongation factor gene, influences yield-related traits in bread wheat (Triticum aestivum L.). Journal of Experimental Botany, 65, 5351–5365.Zhu X, Li Z, Xu H, Zhou M, Du L, Zhang Z. 2012. Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat. Functional & Integrative Genomics, 12, 481–488. |
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