Map-based cloning of qLPA01.1, a favorable allele from Gossypium tomentosum chromosome segment line

doi:10.1016/j.jia.2024.02.011

Journal of Integrative Agriculture

2024, Vol. 23

Issue (10): 3283-3293 DOI: 10.1016/j.jia.2024.02.011

Section 1: Cotton functional genomics

Advanced Online Publication | Current Issue | Archive | Adv Search

Map-based cloning of qLP_A01_.₁, a favorable allele from Gossypium tomentosum chromosome segment line

Wenwen Wang^*, Lei Chen^*, Yan Wu, Xin Guo, Jinming Yang, Dexin Liu, Xueying Liu, Kai Guo, Dajun Liu, Zhonghua Teng, Yuehua Xiao, Zhengsheng Zhang^#

Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400716, China

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摘要

棉花是世界上重要的天然纤维作物，在我们的日常生活中起着至关重要的作用。提高棉花产量一直是棉花育种的一个重要目标。衣分是决定棉花纤维产量的重要因素之一。在前期研究中，我们利用毛棉染色体片段代换系在A01染色体上初步定位到一个控制衣分的稳定QTL，qLP_A01.1。为了精细定位qLP_A01.1，本研究通过将毛棉染色体片段代换系HT_390与陆地棉栽培品种CCRI35杂交，建立了一个包含986个单株的F₂群体。在QTL区域构建了一张包含47个位点、全长为56.98-cM的高密度遗传图谱，并将qLP_A01.1定位到A01染色体上约80kb的区间内，该区间包含6个注释基因。结合转录组和序列分析结果表明， qLP_A01.1的目标基因是硫-酰基转移酶蛋白24 (GoPAT24)。这些结果有助于通过标记辅助选择(MAS)提高棉纤维产量，同时也有利于棉花纤维发育机制的解析。

Abstract

Cotton is an important natural fiber crop worldwide which plays a vital role in our daily life. High yield is a constant goal of cotton breeding, and lint percentage (LP) is one of the important components of cotton fiber yield. A stable QTL controlling LP, qLP_A01_.₁,was identified on chromosome A01 from Gossypium hirsutum introgressed lines with G. tomentosum chromosome segments in a previous study. To fine-map qLP_A01_.₁, an F₂ population with 986 individuals was established by crossing G. hirsutum cultivar CCRI35 with the chromosome segment substitution line HT_390. A high-resolution genetic map including 47 loci and spanning 56.98 cM was constructed in the QTL region, and qLP_A01_.₁ was ultimately mapped into an interval corresponding to an ~80 kb genome region of chromosome A01 in the reference genome, which contained six annotated genes. Transcriptome data and sequence analysis revealed that S-acyltransferase protein 24 (GoPAT24) might be the target gene of qLP_A01_.₁.This result provides the basis for cotton fiber yield improvement via marker-assisted selection (MAS) and further studies on the mechanism of cotton fiber development.

Keywords: cotton lint percentage fine-mapping qLP_A01.₁ GoPAT24

Received: 23 October 2023 Accepted: 18 December 2023

Fund:

This work was supported by the National Natural Science Foundation of China (32172064 and 32201827).

About author: Wenwen Wang, E-mail: www20216024@swu.edu.cn; #Correspondence Zhengsheng Zhang, Tel: +86-23-68250453, E-mail: zhangzs@swu.edu.cn * These authors contributed equally to this study.

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	Wenwen Wang
	Lei Chen
	Yan Wu
	Xin Guo
	Jinming Yang
	Dexin Liu
	Xueying Liu
	Kai Guo
	Dajun Liu
	Zhonghua Teng
	Yuehua Xiao
	Zhengsheng Zhang

Cite this article:

Wenwen Wang, Lei Chen, Yan Wu, Xin Guo, Jinming Yang, Dexin Liu, Xueying Liu, Kai Guo, Dajun Liu, Zhonghua Teng, Yuehua Xiao, Zhengsheng Zhang. 2024. Map-based cloning of qLP_A01_.₁, a favorable allele from Gossypium tomentosum chromosome segment line. Journal of Integrative Agriculture, 23(10): 3283-3293.

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