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Special Issue:
动物科学合辑Animal Science
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| UBE2I stimulates female gonadal differentiation in chicken (Gallus gallus) embryos |
JIN Kai1, 2, 3*, ZHOU Jing1, 2, 3*, ZUO Qi-sheng1, 2, 3, LI Jian-cheng1, 2, 3, Jiuzhou SONG4, ZHANG Ya-ni1, 2, 3, CHANG Guo-bing1, 2, 3, CHEN Guo-hong1, 2, 3, LI Bi-chun1, 2, 3 |
1 Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
2 Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, P.R.China
3 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China
4 Animal & Avian Sciences, University of Maryland, College Park, MD, 20741, USA |
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摘要
该研究报道了一个新的雌性特异表达基因UBE2I，鉴定了其在鸡两性组织器官中的表达模式，并通过体内实验（干扰和过表达）研究了UBE2I在鸡胚性腺发育中的生物学功能。研究发现，UBE2I在鸡早期胚胎性腺中的PGCs（原始生殖细胞）中高表达，并在新生鸡卵巢组织中表现出高表达。与此同时，研究团队结合慢病毒和鸡胚血管注射技术建立了在鸡胚体内长期稳定干扰或过表达UBE2I表达的有效方法，并发现在UBE2I过表达的情况下，雌性的性别相关基因（FOXL2，CYP19A1和HINTW）被上调，而雄性的性别相关基因（SOX9，DMRT1和WT1）在干扰UBE2I后被下调，其对应的鸡胚性腺的组织也出现了对应的结构变化。与此同时，UBE2I表达的变化与雌二醇及其受体（AR和ESR）的水平有关，这些结果表明UBE2I是启动雌性鸡胚卵巢发育的关键因素。该研究结果有力的证明了UBE2I在鸡胚胎性别发育和分化过程中发挥了关键的作用。这是第一次报道UBE2I基因在鸡胚性别分化过程中发挥的功能，而UBE2I作为泛素化修饰过程中的重要调控因子，为进一步研究家禽性别决定的分子机理提供了新的思路。
Abstract Without known analogous sex-determining factors like SRY (sex determining region Y) in mammals, the chicken (Gallus gallus) sex determination mechanism still remains unclear, which highly restricts the biological research on chicken development and poultry single-sex reproduction. Here we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR, but also described the functional role of UBE2I in the gonadal development of chicken embryos. Results showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs (primordial germ cells) in embryonic gonads and robust expression in ovaries of newborn chickens. Most importantly, we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular injection. The qRT-PCR analysis showed that the sex-related genes (FOXL2, CYP19A1 and HINTW) in females were upregulated (P<0.05) under the overexpression of UBE2I and the sex-related genes (SOX9, DMRT1 and WT1) in females were downregulated (P<0.05) after interfering UBE2I. Furthermore, the change of UBE2I expression was associated with the level of estradiol and its receptors (AR and ESR), which suggests that UBE2I is necessary to initiate the female-specific development in chickens. In conclusion, this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens, which provides insights for further understanding the mechanism of sex determination in chickens.
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Received: 09 May 2020
Accepted:
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| Fund: This work was funded by the National Natural Science Foundation of China (31772582 and 31972547), the National Key R&D Program of China (2017YFE0108000), the High Level Talents Support Program of Yangzhou University, Postgraduate Research & Practice Innovation Program of Jiangsu Province, China (KYCX18_2376), the Jiangsu Science and Technology Project, China (Youth Fund, BK20180918), and the Natural Science Research Project of Jiangsu Higher Education Institutions, China (18KJB230008). |
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Corresponding Authors:
Correspondence LI Bi-chun, Tel: +86-514-87977207, Fax: +86-514-87350440, E-mail: yubcli@yzu.edu.cn
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| About author:
JIN Kai, E-mail: jinkai0621@163.com; * These authors contributed equally to this study. |
Cite this article:
JIN Kai, ZHOU Jing, ZUO Qi-sheng, LI Jian-cheng, Jiuzhou SONG, ZHANG Ya-ni, CHANG Guo-bing, CHEN Guo-hong, LI Bi-chun.
2021.
UBE2I stimulates female gonadal differentiation in chicken (Gallus gallus) embryos. Journal of Integrative Agriculture, 20(11): 2986-2994.
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