|Role of TRP channels and HSPs in thermal stress response in the aphid parasitoid Aphelinus asychis (Hymenoptera: Aphelinidae)
LIU Xiang1, KANG Zhi-wei2, YU Xing-lin2, LI Fan2, LIU Tong-xian2, LI Qiang1
1 Department of Entomology, College of Plant Protection, Yunnan Agricultural University, Kunming 650000, P.R.China
2 State Key Laboratory of Crop Stress Biology for Arid Areas/Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling 712100, P.R.China
Aphelinus asychis is an important aphid endoparasitoid. Under field and greenhouse conditions, high temperature is one of the factors limiting the application of A. asychis for biological pest control. To explore the potential role of transient receptor potential (TRP) channels and heat shock proteins (HSPs) in this process, we identified 11 genes encoding TRP channels and nine genes encoding HSPs. Three proteins (AasyTRPA5, AasyPyrexia, AasyPainless) that belong to transient receptor potential ankyrin (TRPA) subfamily and nine HSPs are involved in the response to high temperature. We also investigated the survival of A. asychis and the response of the identified TRP channels and HSPs to high temperature. The results showed that the maximum temperature that allowed A. asychis survival was approximately 41°C; females had higher survival rates than that of the males at 40 and 41°C. Short-term heat-shock resulted in increased expression of Aasyshsp in males, and Aasyhsp40, Aasyhsp68, Aasyhsp70-4, Aasyhsp70-5 and Aasyhsp90 were upregulated and then downregulated, whereas Aasyhsp70-3 was upregulated at 41°C. Moreover, Aasyhsp40 and Aasyhsp90 showed higher expression levels in females, while Aasyshsp and Aasyhsp70-3 presented opposite expression patterns. At temperature above 35°C, expression of AasyPyrexia in females was significant higher than that in males, whereas AasyPainless and AasyTRPA5 presented higher expression in males at 40 and 41°C, respectively. Altogether, these results indicate that protection against thermal stress in A. asychis is coordinated by TRP channels and HSPs. These findings provide a basis for understanding the potential mechanism of A. asychis in response to high temperatures.
Received: 23 May 2019
|Fund: This work was supported by the National Key Basic Research Program of China (973 Program; 2013CB127600)
Correspondence LIU Tong-xian, E-mail: email@example.com; LI Qiang, E-mail: firstname.lastname@example.org
|About author: LIU Xiang, Mobile: +86-18700907734, E-mail: email@example.com;
Cite this article:
LIU Xiang, KANG Zhi-wei, YU Xing-lin, LI Fan, LIU Tong-xian, LI Qiang .
Role of TRP channels and HSPs in thermal stress response in the aphid parasitoid Aphelinus asychis (Hymenoptera: Aphelinidae). Journal of Integrative Agriculture, 19(6): 1530-1542.
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