Please wait a minute...
Journal of Integrative Agriculture  2017, Vol. 16 Issue (07): 1576-1591    DOI: 10.1016/S2095-3119(16)61521-6
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
In silico genome-wide identification, phylogeny and expression analysis of the R2R3-MYB gene family in Medicago truncatula
ZHENG Xing-wei, YI Deng-xia, SHAO Lin-hui, LI Cong
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract      The R2R3-MYB genes make up one of the largest transcription factor families in plants, and play regulatory roles in various biological processes such as development, metabolism and defense response.  Although genome-wide analyses of this gene family have been conducted in several species, R2R3-MYB genes have not been systematically analyzed in Medicago truncatula, a sequenced model legume plant.  Here, we performed a comprehensive, genome-wide computational analysis of the structural characteristics, phylogeny, functions and expression patterns of M. truncatula R2R3-MYB genes.  DNA binding domains are highly conserved among the 155 putative MtR2R3-MYB proteins that we identified.  Chromosomal location analysis revealed that these genes were distributed across all eight chromosomes.  Results showed that the expansion of the MtR2R3-MYB family was mainly attributable to segmental duplication and tandem duplication.  A comprehensive classification was performed based on phylogenetic analysis of the R2R3-MYB gene families in M. truncatula, Arabidopsis thaliana and other plant species.  Evolutionary relationships within clades were supported by clade-specific conserved motifs outside the MYB domain.  Species-specific clades have been gained or lost during evolution, resulting in functional divergence.  Also, tissue-specific expression patterns were investigated.  The functions of stress response-related clades were further verified by the changes in transcript levels of representative R2R3-MYB genes upon treatment with abiotic and biotic stresses.  This study is the first report on identification and characterization of R2R3-MYB gene family based on the genome of M. truncatula, and will facilitate functional analysis of this gene family in the future.  
Keywords:  R2R3-MYB        Medicago truncatula        gene family        stress response        function prediction  
Received: 03 June 2016   Accepted:

This work was supported by the National Natural Science Foundation of China (31372362).

Corresponding Authors:  Correspondence LI Cong, Tel: +86-10-62815998, E-mail:    
About author:  ZHENG Xing-wei, E-mail:;

Cite this article: 

ZHENG Xing-wei, YI Deng-xia, SHAO Lin-hui, LI Cong . 2017. In silico genome-wide identification, phylogeny and expression analysis of the R2R3-MYB gene family in Medicago truncatula. Journal of Integrative Agriculture, 16(07): 1576-1591.

Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2003. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. The Plant Cell, 15, 63–78.

Albert N W. 2015. Subspecialization of R2R3-MYB repressors for anthocyanin and proanthocyanidin regulation in forage legumes. Frontiers in Plant Science, 6, 13.

Ambawat S, Sharma P, Yadav N R, Yadav R C. 2013. MYB transcription factor genes as regulators for plant responses: An overview. Physiology and Molecular Biology of Plants, 19, 307–321.

Bailey T L, Elkan C. 1994. Fitting a mixture model by expectation maximization to discover motifs in bipolymers. International Conference on Intelligent Systems for Molecular Biology, 2, 28–36.

Baldoni E, Genga A, Cominelli E. 2015. Plant MYB transcription factors: Their role in drought response mechanisms. International Journal of Molecular Sciences, 16, 15811–15851.

Baumann K, Perez-Rodriguez M, Bradley D, Venail J, Bailey P, Jin H L, Koes R, Roberts K, Martin C. 2007. Control of cell and petal morphogenesis by R2R3 MYB transcription factors. Development, 134, 1691–1701.

Berardini T Z, Reiser L, Li D H, Mezheritsky Y, Muller R, Strait E, Huala E. 2015. The arabidopsis information resource: Making and mining the “gold standard” annotated reference plant genome. Genesis, 53, 474–485.

Bonthala V S, Mayes K, Moreton J, Blythe M, Wright V, May S T, Massawe F, Mayes S, Twycross J. 2016. Identification of gene modules associated with low temperatures response in Bambara groundnut by network-based analysis. PLoS ONE, 11, doi: 10.1371/journal.pone.0148771

Braun E L, Grotewold E. 1999. Newly discovered plant c-myb-like genes rewrite the evolution of the plant myb gene family. Plant Physiology, 121, 21–24.

Carletti G, Lucini L, Busconi M, Marocco A, Bernardi J. 2013. Insight into the role of anthocyanin biosynthesis-related genes in Medicago truncatula mutants impaired in pigmentation in leaves. Plant Physiology and Biochemistry, 70, 123–132.

Cavallini E. 2014. Functional diversification of grapevine MYB5a and MYB5b in the control of flavonoid biosynthesis in a petunia anthocyanin regulatory mutant. Plant & Cell Physiology, 55, 517–534.

Chai G, Wang Z, Tang X, Yu L, Qi G, Wang D, Yan X, Kong Y, Zhou G. 2014. R2R3-MYB gene pairs in Populus: Evolution and contribution to secondary wall formation and flowering time. Journal of Experimental Botany, 65, 4255–4269.

Deng W, Wang Y, Liu Z, Cheng H, Xue Y. 2014. HemI: A toolkit for illustrating heatmaps. PLoS ONE, 9, doi: 10.1371/journal.pone.0111988

Du H, Feng B R, Yang S S, Huang Y B, Tang Y X. 2012a. The R2R3-MYB transcription factor gene family in maize. PLoS ONE, 7, doi: 10.1371/journal.pone.0037463

Du H, Liang Z, Zhao S, Nan M G, Tran L S P, Lu K, Huang Y B, Li J N. 2015. The evolutionary history of R2R3-MYB proteins across 50 eukaryotes: New insights into subfamily classification and expansion. Scientific Reports, 5, 16.

Du H, Yang S S, Liang Z, Feng B R, Liu L, Huang Y B, Tang Y X. 2012b. Genome-wide analysis of the MYB transcription factor superfamily in soybean. BMC Plant Biology, 12, 1–22.

Du H, Zhang L, Liu L, Tang X F, Yang W J, Wu Y M, Huang Y B, Tang Y X. 2009. Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry, 74, 5–16.

Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. 2010. MYB transcription factors in Arabidopsis. Trends in Plant Science, 15, 573–581.

Feng S, Xu Y, Yang L, Sun S, Wang D, Chen X. 2015. Genome-wide identification and characterization of R2R3-MYB transcription factors in pear. Scientia Horticulturae, 197, 176–182.

Finn R D, Coggill P, Eberhardt R Y, Eddy S R, Mistry J, Mitchell A L, Potter S C, Punta M, Qureshi M, Sangrador-Vegas A, Salazar G A, Tate J, Bateman A. 2016. The Pfam protein families database: Towards a more sustainable future. Nucleic Acids Research, 44, D279–D285.

Franco-Zorrilla J M, Lopez-Vidriero I, Carrasco J L, Godoy M, Vera P, Solano R. 2014. DNA-binding specificities of plant transcription factors and their potential to define target genes. Proceedings of the National Academy of Sciences of the United States of America, 111, 2367–2372.

Geethalakshmi S, Barathkumar S, Prabu G. 2015. The MYB transcription factor family genes in sugarcane (Saccharum sp.). Plant Molecular Biology Reporter, 33, 512–531.

Gocal G F W, Poole A T, Gubler F, Watts R J, Blundell C, King R W. 1999. Long-day up-regulation of a GAMYB gene during Lolium temulentum inflorescence formation. Plant Physiology, 119, 1271–1278.

He Q L, Jones D C, Li W, Xie F L, Ma J, Sun R R, Wang Q L, Zhu S J, Zhang B H. 2016. Genome-wide identification of R2R3-MYB genes and expression analyses during abiotic stress in Gossypium raimondii. Scientific Reports, 6, 22980.

Hall B G. 2013. Building phylogenetic trees from molecular data with MEGA. Molecular Biology and Evolution, 30, 1229–1235.

Haga N, Kato K, Murase M, Araki S, Kubo M, Demura T, Suzuki K, Muller I, Voss U, Jurgens G, Ito M. 2007. R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana. Development, 134, 1101–1110.

Hichri I, Barrieu F, Bogs J, Kappel C, Delrot S, Lauvergeat V. 2011. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. Journal of Experimental Botany, 62, 2465–2483.

Hou X J, Li S B, Liu S R, Hu C G, Zhang J Z. 2014. Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange. PLoS ONE, 9, doi: 10.1371/journal.pone.0112375

Huang W J, Sun W, Lv H Y, Luo M, Zeng S H, Pattanaik S, Yuan L, Wang Y. 2013. A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway. PLoS ONE, 8, doi: 10.1371/journal.pone.0070778

Ito M, Araki S, Matsunaga S, Itoh T, Nishihama R, Machida Y, Doonan J H, Watanabe A. 2001. G2/M-phase-special transcription during the plant cell cycle is mediated by c-Myb-like transcription factors. The Plant Cell, 13, 1891–1905.

Jia L, Clegg M T, Jiang T. 2004. Evolutionary dynamics of the DNA-binding domains in putative R2R3-MYB genes identified from rice subspecies indica and japonica genomes. Plant Physiology, 134, 575–585.

Jiang C H, Gu J Y, Chopra S, Gu X, Peterson T. 2004. Ordered origin of the typical two- and three-repeat Myb genes. Gene, 326, 13–22.

Jin H L, Martin C. 1999. Multifunctionality and diversity within the plant MYB-gene family. Plant Molecular Biology, 41, 577–585.

Jung C, Seo J S, Han S W, Koo Y J, Kim C H, Song S I, Nahm B H, Choi Y D, Cheong J J. 2008. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiology, 146, 623–635.

Katiyar A, Smita S, Lenka S K, Rajwanshi R, Chinnusamy V, Bansal K C. 2012. Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis. BMC Genomics, 13, 1–19.

Kelemen Z, Sebastian A, Xu W J, Grain D, Salsac F, Avon A, Berger N, Tran J, Dubreucq B, Lurin C, Lepiniec L, Contreras-Moreira B, Dubos C. 2015. Analysis of the DNA-binding activities of the Arabidopsis R2R3-MYB transcription factor family by one-hybrid experiments in yeast. PLoS ONE, 10, doi: 10.1371/journal.pone.0141044

Kim J H, Nguyen N H, Jeong C Y, Nguyen N T, Hong S W, Lee H. 2013. Loss of the R2R3 MYB, AtMyb73, causes hyper-induction of the SOS1 and SOS3 genes in response to high salinity in Arabidopsis. Journal of Plant Physiology, 170, 1461–1465.

Kim J, Yang J Y, Yang R H, Sicher R C, Chang C, Tucker M L. 2016. Transcriptome analysis of soybean leaf abscission identifies transcriptional regulators of organ polarity and cell fate. Frontiers in Plant Science, 7, 16.

Kohler A, Rinaldi C, Duplessis S, Baucher M, Geelen D, Duchaussoy F, Meyers B C, Boerjan W, Martin F. 2008. Genome-wide identification of NBS resistance genes in Populus trichocarpa. Plant Molecular Biology, 66, 619–636.

Kranz H D, Denekamp M, Greco R, Jin H, Leyva A, Meissner R C, Petroni K, Urzainqui A, Bevan M, Martin C, Smeekens S, Tonelli C, Paz-Ares J, Weisshaar B. 1998. Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. The Plant Journal, 16, 263–276.

Lau S, Schwarzacher T, Rofina Yasmin O, Harikrishna J A. 2015. dsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid. BMC Plant Biology, 15, 1–14.

Legay S, Sivadon P, Blervacq A S, Pavy N, Baghdady A, Tremblay L, Levasseur C, Ladouce N, Lapierre C, Seguin A, Hawkins S, Mackay J, Grima-Pettenati J. 2010. EgMYB1, an R2R3 MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar. New Phytologist, 188, 774–786.

Li C, Ng C K Y, Fan L M. 2015. MYB transcription factors, active players in abiotic stress signaling. Environmental and Experimental Botany, 114, 80–91.

Li J, Yang X, Wang Y, Li X, Gao Z, Pei M, Chen Z, Qu L J, Gu H. 2006. Two groups of MYB transcription factors share a motif which enhances trans-activation activity. Biochemical and Biophysical Research Communications, 341, 1155–1163.

Li X, Xue C, Li J, Qiao X, Li L, Yu L a, Huang Y, Wu J. 2016. Genome-wide identification, evolution and functional divergence of MYB transcription factors in Chinese white pear (Pyrus bretschneideri). Plant and Cell Physiology, 57, 824–847.

Liao Y, Zou H F, Wang H W, Zhang W K, Ma B, Zhang J S, Chen S Y. 2008. Soybean GmMYB76, GmMYB92 and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants. Cell Research, 18, 1047–1060.

Lin Y X, Cheng Y, Jin J, Jin X L, Jiang H Y, Yan H W, Cheng B J. 2014. Genome duplication and gene loss affect the evolution of heat shock transcription factor genes in Legumes. PLoS ONE, 9, doi: 10.1371/journal.pone.0102825

Lipsick J S. 1996. One billion years of Myb. Oncogene, 13, 223–235.

Liu J, Osbourn A, Ma P. 2015. MYB transcription factors as regulators of phenylpropanoid metabolism in plants. Molecular Plant, 8, 689–708.

Liu Z, Luan Y S, Li J B, Yin Y L. 2016. Expression of a tomato MYB gene in transgenic tobacco increases resistance to Fusarium oxysporum and Botrytis cinerea. European Journal of Plant Pathology, 144, 607–617.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2–??CT method. Methods, 25, 402–408.

Ma Q H, Wang C, Zhu H H. 2011. TaMYB4 cloned from wheat regulates lignin biosynthesis through negatively controlling the transcripts of both cinnamyl alcohol dehydrogenase and cinnamoyl-CoA reductase genes. Biochimie, 93, 1179–1186.

Martin C, Paz-Ares J. 1997. MYB transcription factors in plants. Trends in Genetics, 13, 67–73.

Matus J T, Aquea F, Arce-Johnson P. 2008. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes. BMC Plant Biology, 8, 83.

Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. 2012. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta (BBA, Gene Regulatory Mechanisms), 1819, 86–96.

Muller D, Schmitz G, Theres K. 2006. Blind homologous R2R3 Myb genes control the pattern of lateral meristem initiation in Arabidopsis. The Plant Cell, 18, 586–597.

Nuruzzaman M, Manimekalai R, Sharoni A, Satoh K, Kondoh H, Ooka H, Kikuchi S. 2010. Genome-wide analysis of NAC transcription factor family in rice. Gene, 465, 30–44.

Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kanai H, Sarai A, Ishii S, Nishimura Y. 1994. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices. Cell, 79, 639–648.

Panchy N, Shiu M L, Shiu S H. 2016. Evolution of gene duplication in plants. Plant Physiology, 171, 2294–2316.

Passeri V, Koes R, Quattrocchio F M. 2016. New challenges for the design of high value plant products: Stabilization of anthocyanins in plant vacuoles. Frontiers in Plant Science, 7, 9.

Paz-Ares J, Ghosal D, Wienand U, Peterson P A, Saedler H. 1987. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. Embo Journal, 6, 3553–3558.

Peel G J, Pang Y, Modolo L V, Dixon R A. 2009. The LAP1 MYB transcription factor orchestrates anthocyanidin biosynthesis and glycosylation in Medicago. The Plant Journal, 59, 136–149.

Perez-Diaz J R, Perez-Diaz J, Madrid-Espinoza J, Gonzalez-Villanueva E, Moreno Y, Ruiz-Lara S. 2016. New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in the flowers of transgenic tobacco. Plant Molecular Biology, 90, 63–76.

Perez-Rodriguez M, Jaffe F W, Butelli E, Glover B J, Martin C. 2005. Development of three different cell types is associated with the activity of a specific MYB transcription factor in the ventral petal of Antirrhinum majus flowers. Development, 132, 359–370.

Qin C, Cheng L, Shen J, Zhang Y, Cao H, Lu D, Shen C. 2016. Genome-wide identification and expression analysis of the 14-3-3 family genes in Medicago truncatula. Frontiers in Plant Science, 7, 320.

Riechmann J L, Heard J, Martin G, Reuber L, Jiang C Z, Keddie J, Adam L, Pineda O, Ratcliffe O J, Samaha R R, Creelman R, Pilgrim M, Broun P, Zhang J Z, Ghandehari D, Sherman B K, Yu G L. 2000. Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes. Science, 290, 2105–2110.

Sagawa J M, Stanley L E, LaFountain A M, Frank H A, Liu C, Yuan Y. 2016. An R2R3-MYB transcription factor regulates carotenoid pigmentation in Mimulus lewisii flowers. New Phytologist, 209, 1049–1057.

Schmutz J, Cannon S B, Schlueter J, Ma J, Mitros T, Nelson W, Hyten D L, Song Q, Thelen J J, Cheng J, Xu D, Hellsten U, May G D, Yu Y, Sakurai T, Umezawa T, Bhattacharyya M K, Sandhu D, Valliyodan B, Lindquist E, et al. 2010. Genome sequence of the palaeopolyploid soybean. Nature, 463, 178–183.

Sharoni A M, Nuruzzaman M, Satoh K, Shimizu T, Kondoh H, Sasaya T, Choi I R, Omura T, Kikuchi S. 2011. Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant and Cell Physiology, 52, 344–360.

Shin J H, Van K, Kim D H, Kim K D, Jang Y E, Choi B S, Kim M Y, Lee S H. 2008. The lipoxygenase gene family: A genomic fossil of shared polyploidy between Glycine max and Medicago truncatula. BMC Plant Biology, 8, 133.

Shoemaker R C, Schlueter J, Doyle J J. 2006. polyploidy and gene duplication in soybean and other legumes. Current Opinion in Plant Biology, 9, 104–109.

Soler M, Camargo E L O, Carocha V, Cassan-Wang H, San Clemente H, Savelli B, Hefer C A, Paiva J A P, Myburg A A, Grima-Pettenati J. 2015. The Eucalyptus grandis R2R3-MYB transcription factor family: Evidence for woody growth-related evolution and function. New Phytologist, 206, 1364–1377.

Song H, Nan Z B. 2014. Genome-wide identification and analysis of WRKY transcription factors in Medicago truncatula. Hereditas, 36, 152–168. (in Chinese)

Song S S, Qi T C, Huang H, Ren Q C, Wu D W, Chang C Q, Peng W, Liu Y L, Peng J R, Xie D X. 2011. The jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect jasmonate-regulated stamen development in Arabidopsis. The Plant Cell, 23, 1000–1013.

Stracke R, Holtgrawe D, Schneider J, Pucker B, Sorensen T R, Weisshaar B. 2014. Genome-wide identification and characterisation of R2R3-MYB genes in sugar beet (Beta vulgaris). BMC Plant Biology, 14, 17.

Stracke R, Werber M, Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 4, 447–456.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.

Uppalapati S R, Marek S M, Lee H-K, Nakashima J, Tang Y, Sledge M K, Dixon R A, Mysore K S. 2008. Global gene expression profiling during Medicago truncatula-Phymatotrichopsis omnivora interaction reveals a role for jasmonic acid, ethylene, and the flavonoid pathway in disease development. Molecular Plant-Microbe Interactions, 22, 7–17.

Walther D, Brunnemann R, Selbig J. 2007. The regulatory code for transcriptional response diversity and its relation to genome structural properties in A. thaliana. PLoS Genetics, 3, 216–229.

Wang H Y, Wang H L, Shao H B, Tang X L. 2016. Recent advances in utilizing transcription factors to improve plant abiotic stress tolerance by transgenic technology. Frontiers in Plant Science, 7, 13.

Wang W, Li E Y, Porth I, Chen J G, Mansfield S, Douglas C, Wang S C. 2016. Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis. Journal of Plant Biology, 59, 16–23.

Wilkins O, Nahal H, Foong J, Provart N J, Campbell M M. 2009. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiology, 149, 981–993.

Xu G, Huang J, Yang Y, Yao Y A. 2016. Transcriptome analysis of flower sex differentiation in Jatropha curcas L. using RNA sequencing. PLoS ONE, 11, doi: 10.1371/journal.pone.0145613

Yang A, Dai X, Zhang W H. 2012. An R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. Journal of Experimental Botany, 63, 2541–2556.

Young N D, Debelle F, Oldroyd G E D, Geurts R, Cannon S B, Udvardi M K, Benedito V A, Mayer K F X, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook D R, Meyers B C, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S G, et al. 2011. The Medicago genome provides insight into the evolution of rhizobial symbioses. Nature, 480, 520–524.

Yu Y T, Wu Z, Lu K, Bi C, Liang S, Wang X F, Zhang D P. 2016. Overexpression of the MYB37 transcription factor enhances abscisic acid sensitivity, and improves both drought tolerance and seed productivity in Arabidopsis thaliana. Plant Molecular Biology, 90, 267–279.

Zhang W, Han Z X, Guo Q L, Liu Y, Zheng Y X, Wu F L, Jin W B. 2014. Identification of maize long non-coding RNAs responsive to drought stress. PLoS ONE, 9, doi: 10.1371/journal.pone.0098958

Zhang Z Y, Liu X, Wang X D, Zhou M P, Zhou X Y, Ye X G, Wei X N. 2012. An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. New Phytologist, 196, 1155–1170.

Zheng K J, Tian H N, Hu Q N, Guo H Y, Yang L, Cai L, Wang X T, Liu B, Wang S C. 2016. Ectopic expression of R3 MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation. Scientific Reports, 6, 12.

Zhou M L, Hou H L, Zhu X M, Shao J R, Wu Y M, Tang Y X. 2011. Soybean transcription factor GmMYBZ2 represses catharanthine biosynthesis in hairy roots of Catharanthus roseus. Applied Microbiology and Biotechnology, 91, 1095–1105.
[1] CHEN Rong-zhu, SHEN Xu, ZHANG Shu-ting, ZHAO Hua, CHEN Xiao-hui, XU Xiao-ping, HUO Wen, ZHANG Zi-hao, LIN Yu-ling, LAI Zhong-xiong. Genome-wide identification and expression analysis of Argonaute gene family from longan embryogenic callus[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2138-2155.
[2] SHI Dong-ya, REN Wei-chao, WANG Jin, ZHANG Jie, Jane Ifunanya MBADIANYA, MAO Xue-wei, CHEN Chang-jun. The transcription factor FgNsf1 regulates fungal development, virulence and stress responses in Fusarium graminearum[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2156-2169.
[3] XIONG Wen-feng, XIE Jia, WEI Lu-ting, ZHANG Si-si, SONG Xiao-wen, GAO Shan-shan, LI Bin. Transcriptome analysis of hsp18.3 functions and regulatory systems using RNA-sequencing in the red flour beetle, Tribolium castaneum[J]. >Journal of Integrative Agriculture, 2018, 17(05): 1040-1056.
No Suggested Reading articles found!