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Journal of Integrative Agriculture  2020, Vol. 19 Issue (2): 449-464    DOI: 10.1016/S2095-3119(19)62660-2
Special Issue: Maize Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing
HAO Lu-yang1*, LIU Xu-yang1*, ZHANG Xiao-jing1, SUN Bao-cheng2, LIU Cheng2, ZHANG Deng-feng1, TANG Huai-jun2, LI Chun-hui1, LI Yong-xiang1, SHI Yun-su1, XIE Xiao-qing2, SONG Yan-chun1, WANG Tian-yu1, LI Yu1  
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830002, P.R.China
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Abstract  
Drought is one of the most important abiotic stresses affecting maize growth and development and therefore resulting in yield loss.  Thus it is essential to understand molecular mechanisms of drought stress responses in maize for drought tolerance improvement.  The root plays a critical role in plants sensing water deficit.  In the present study, two maize inbred lines, H082183, a drought-tolerant line, and Lv28, a drought-sensitive line, were grown in the field and treated with different water conditions (moderate drought, severe drought, and well-watered conditions) during vegetative stage.  The transcriptomes of their roots were investigated by RNA sequencing.  There were 1 428 and 512 drought-responsive genes (DRGs) in Lv28, 688 and 3 363 DRGs in H082183 under moderate drought and severe drought, respectively.  A total of 31 Gene Ontology (GO) terms were significantly over-represented in the two lines, 13 of which were enriched only in the DRGs of H082183.  Based on results of Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis, “plant hormone signal transduction” and “starch and sucrose metabolism” were enriched in both of the two lines, while “phenylpropanoid biosynthesis” was only enriched in H082183.  Further analysis revealed the different expression patterns of genes related to abscisic acid (ABA) signal pathway, trehalose biosynthesis, reactive oxygen scavenging, and transcription factors might contribute to drought tolerance in maize.  Our results contribute to illustrating drought-responsive molecular mechanisms and providing gene resources for maize drought improvement.
Keywords:  maize (Zea mays L.)        root        transcriptome        RNA sequencing        drought-responsive genes  
Received: 10 October 2018   Accepted: 18 January 2020
Fund: This work was supported by the Sci-Tech Innovation Program of Chinese Academy of Agricultural Sciences (Y2016PT10).
Corresponding Authors:  Correspondence WANG Tian-yu, E-mail: wangtianyu@caas.cn; LI Yu, E-mail: liyu03@caas.cn    
About author:  Received 10 October, 2018 Accepted 1 March, 2019 HAO Lu-yang, E-mail: 841866574@qq.com; LIU Xu-yang, E-mail: 1021247118@qq.com; * These authors contributed equally to this study.

Cite this article: 

HAO Lu-yang, LIU Xu-yang, ZHANG Xiao-jing, SUN Bao-cheng, LIU Cheng, ZHANG Deng-feng, TANG Huai-jun, LI Chun-hui, LI Yong-xiang, SHI Yun-su, XIE Xiao-qing, SONG Yan-chun, WANG Tian-yu, LI Yu . 2020.

Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing
. Journal of Integrative Agriculture, 19(2): 449-464.

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