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Journal of Integrative Agriculture  2020, Vol. 19 Issue (1): 33-50    DOI: 10.1016/S2095-3119(19)62659-6
Special Issue: 小麦遗传育种Wheat Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat
LI Xiao-lan1, 3, LÜ Xiang1, WANG Xiao-hong1, PENG Qin2, ZHANG Ming-sheng1, REN Ming-jian2 
1 School of Life Sciences, Guizhou University/State Engineering Technology Institute for Karst Desertification Control/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang 550025, P.R.China
2 School of Agriculture, Guizhou University/Guizhou Sub-Center of National Wheat Improvement Center, Guiyang 550025, P.R.China
3 Special Key Laboratory of Microbial Resources and Drug Development from Higher Education Institution of Guizhou Province/Research Center for Medicine and Biology, Zunyi Medical University, Zunyi 563006, P.R.China
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Abstract  
Triticum aestivum L. cv. Guizi 1 (GZ1) is a drought-tolerant local purple wheat cultivar.  It is not clear how purple wheat resists drought stress, but it could be related to anthocyanin biosynthesis.  In this study, transcriptome data from drought-treated samples and controls were compared.  Drought slightly reduced the anthocyanin, protein and starch contents of GZ1 grains and significantly reduced the grain weight. Under drought stress, 16 682 transcripts were reduced, 27 766 differentially expressed genes (DEGs) were identified, and 379 DEGs, including DREBs, were related to defense response.  The defense-response genes included response to water deprivation, reactive oxygen, bacteria, fungi, etc.  Most of the structural and regulatory genes in anthocyanin biosynthesis were downregulated, with only TaDFR, TaOMT, Ta5,3GT, and TaMYB-4B1 being upregulated. TaCHS, TaF3H, TaCHI, Ta4CL, and TaF3’H are involved in responses to UV, hormones, and stimulus.  TaCHS-2D1, TaDFR-2D2, TaDFR-7D, TaOMT-5A, Ta5,3GT-1B1, Ta5,3GT-3A, and Ta5,3GT-7B1 connect anthocyanin biosynthesis with other pathways, and their interacting proteins are involved in primary metabolism, genetic regulation, growth and development, and defense responses.  There is further speculation about the defense-responsive network in purple wheat.  The results indicated that biotic and abiotic stress-responsive genes were stimulated to resist drought stress in purple wheat GZ1, and anthocyanin biosynthesis also participated in the drought defense response through several structural genes.
Keywords:  transcriptome        purple wheat, drought        anthocyanin       differentially expressed genes       defense response       stress  
Received: 23 September 2018   Accepted:
Fund: This study was supported by the grants from the National Key R&D Program of China (2017YFD0100901-4 and 2016YFC0502604), the National Natural Science Foundation of China (31660390), the Major Special Project of Science and Technology Program in Guizhou, China (2017-5411-06 and 2017-5788), the Construction Project of State Engineering Technology Institute for Karst Desertification Control, China (2012FU125X13), the Innovation Talents Team Construction of Science and Technology in Guizhou, China (2016-5624), the Major Research Project of Innovation Group in Guizhou, China (2016-023), and the Graduate Innovation Fund of Guizhou University, China (2017025), and the Science and Technology Project in Guizhou, China (2019-4246).
Corresponding Authors:  Correspondence REN Ming-jian, Tel: +86-851-83855894, E-mail: rmj72@163.com    
About author:  LI Xiao-lan, Tel: +86-851-28642444, E-mail: lixiaolanl@163.com;

Cite this article: 

LI Xiao-lan, Lü Xiang, WANG Xiao-hong, PENG Qin, ZHANG Ming-sheng, REN Ming-jian . 2020. Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat. Journal of Integrative Agriculture, 19(1): 33-50.

Abdel-Aal E S M, Hucl P. 1999. A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheat. Cereal Chemistry, 76, 350–354.
Buchfink B, Xie C, Huson D H. 2015. Fast and sensitive protein alignment using DIAMOND. Nature Methods, 12, 59–60.
Cadiz N M, Kwon T R. 2008. Drought and salinity stress interactions: An overview. Asia Life Sciences, 17, 337–347.
Cui Z H, Bi W L, Hao X Y, Li P M, Duan Y, Walker M A, Xu Y, Wang Q C. 2017. Drought stress enhances up-regulation of anthocyanin biosynthesis in Grapevine leafroll-associated virus 3-infected in vitro Grapevine (Vitis vinifera) leaves. Plant Disease, 101, 1606–1615.
Dardick C, Ronald P. 2006. Plant and animal pathogen recognition receptors signal through non-RD kinases. PLoS Pathogens, 2, 0014–0028.
Fang Y, Du Y, Wang J, Wu A, Qiao S, Xu B, Zhang S, Siddique K H M, Chen Y. 2017. Moderate drought stress affected root growth and grain yield in old, modern and newly released cultivars of winter wheat. Frontiers in Plant Science, 8, 672.
Gonzalez-Villagra J, Kurepin L V, Reyes-Diaz M M. 2017. Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants. Planta, 246, 299–312.
Goyal E, Amit S K, Singh R S, Mahato A K, Chand S, Kanika K. 2016. Transcriptome profiling of the salt-stress response in Triticum aestivum cv. Kharchia Local. Scientific Reports, 6, 27752.
Hanaka A, Lechowski L, Mroczek-Zdyrska M, Strubinska J. 2018. Oxidative enzymes activity during abiotic and biotic stresses in Zea mays leaves and roots exposed to Cu, methyl jasmonate, and Trigonotylus caelestialium. Physiology and Molecular Biology of Plants, 24, 1–5.
Jaakola L. 2013. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science, 18, 477–483.
Jazayeri S M, Munoz L M M, Romero H M. 2015. RNA-Seq: A glance at technologies and methodologies. Acta Biologica Colombiana, 20, 23–35.
Jiang W, Liu T, Nan W, Chamila Jeewani D, Niu Y, Li C, Wang Y, Shi X, Wang C, Wang J, Li Y, Gao X, Wang Z. 2018. Two transcription factors TaPpm1 and TaPpb1 co-regulate the anthocyanin biosynthesis in purple pericarp of wheat. Journal of Experimental Botany, 69, 2555–2567
Kim D, Langmead B, Salzberg S L. 2015. HISAT: A fast spliced aligner with low memory requirements. Nature Methods, 12, 357–360.
Kim K H, Kamal A H M, Shin K H, Choi J S, Park C S, Heo H Y, Woo S H. 2010. Wild relatives of the wheat grain proteome. Journal of Plant Biology, 53, 344–357.
Kong L, Zhang Y, Ye Z Q, Liu X Q, Zhao S Q, Wei L, Gao G. 2007. CPC: Assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic Acids Research, 35, W345–W349.
Leng P F, Thomas L, Xu M L. 2017. Genomics-assisted breeding - A revolutionary strategy for crop improvement. Journal of Integrative Agriculture, 16, 2674–2685.
Li B, Dewey C N. 2011. RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics, 12, 323.
Li P, Li Y J, Zhang F J, Zhang G Z, Jiang X Y, Yu H M, Hou B K. 2017. The Arabidopsis UDP-glycosyltransferases UGT79B2 and UGT79B3, contribute to cold, salt and drought stress tolerance via modulating anthocyanin accumulation. The Plant Journal, 89, 85–103.
Li X, Yang X, Hu Y, Yu X, Li Q. 2014. A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress tolerance. Plant Cell Reports, 33, 767–778.
Li X, Zhang M, Ren M, Lü X, Ji N, Wang X. 2017. Molecular regulation mechanism of anthocyanin synthesis in purple wheat. Plant Physiology Journal, 53, 521–530.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(–Delta Delta CT) method. Methods, 25, 402–408
Liu Y, Tikunov Y, Schouten R E, Marcelis L F M, Visser R G F, Bovy A. 2018. Anthocyanin biosynthesis and degradation mechanisms in solanaceous vegetables: A review. Frontiers in Chemistry, 6, 52.
Ma D, Sun D, Wang C, Li Y, Guo T. 2014. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiology and Biochemistry, 80, 60–66.
Mahajan S, Tteja N. 2005. Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444, 139–158.
Marguerat S, Bahler J. 2010. RNA-seq: From technology to biology. Cellular and Molecular Life Sciences, 67, 569–579.
von Mering C, Jensen L J, Snel B, Hooper S D, Krupp M, Foglierini M, Jouffre N, Huynen A M, Bork P. 2005. STRING: Known and predicted protein-protein associations, integrated and transferred across organisms. Nucleic Acids Research, 33, D433–D437.
Park Y S, Bae D W, Ryu C M. 2015. Aboveground whitefly infestation modulates transcriptional levels of anthocyanin biosynthesis and jasmonic acid signaling-related genes and augments the cope with drought stress of maize. PLoS ONE, 10, e0143879.
Pertea M, Pertea G M, Antonescu C M, Chang T C, Mendell J T, Salzberg S L. 2015. StringTie enables improved reconstruction of a transcriptome from RNA-Seq reads. Nature Biotechnology, 33, 290–295.
Ploenlap P, Pattanagul W. 2015. Effects of exogenous abscisic acid on foliar anthocyanin accumulation and drought tolerance in purple rice. Biologia, 70, 915–921.
Sasaki N, Matsuba Y, Abe Y, Okamura M, Momose M, Umemoto N, Nakayama M, Itoh Y, Ozeki Y. 2013. Recent advances in understanding the anthocyanin modification steps in carnation flowers. Scientia Horticulturae, 163, 37–45.
Shen S, Park J W, Lu Z X, Lin L, Henry M D, Wu Y N, Zhou Q, Xing Y. 2014. rMATS: Robust and flexible detection of differential alternative splicing from replicate RNA-Seq data. Proceedings of the National Academy of Sciences of the United States of America, 111, E5593–E5601.
Shi G Q , Fu J Y, Rong L J, Zhang P Y, Guo C J, Xiao K. 2018. TaMIR1119, a miRNA family member of wheat (Triticum aestivum), is essential in the regulation of plant drought tolerance. Journal of Integrative Agriculture, 17, 5–14.
Springob K, Nakajima J, Yamazaki M, Saito K. 2003. Recent advances in the biosynthesis and accumulation of anthocyanins. Natural Product Reports, 20, 288–303.
Teng W, He X, Tong Y P. 2017. Transgenic approaches for improving use efficiency of nitrogen, phosphorus and potassium in crops. Journal of Integrative Agriculture, 16, 2657–2673.
Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley R D, Pimentel H, Salzberg S, Rinn L J, Pachter L. 2012. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols, 7, 562–578.
Uma B, Podile A R. 2015. Apoplastic oxidative defenses during non-host interactions of tomato (Lycopersicon esculentum L.) with Magnaporthe grisea. Acta Physiologiae Plantarum, 37, 26.
Wang F, Dong X, Tang X, Tu L, Zhao B, Sui N, Fu D, Zhang X. 2018. Comparative transcriptome analysis revealing the effect of light on anthocyanin biosynthesis in purple grains of wheat. Journal of Agricultural and Food Chemistry, 66, 3465–3476.
Wang H Q, Wu Y C, Yang X B, Guo X R, Cao X Y. 2017. SmLEA2, a gene for late embryogenesis abundant protein isolated from Salvia miltiorrhiza, confers tolerance to drought and salt stress in Escherichia coli and
S. miltiorrhizo. Protoplasma, 254, 685–696.
Wang J, Pan Y, Shen S, Lin L, Xing Y. 2017. rMATS-DVR: rMATS discovery of differential variants in RNA. Bioinformatics, 33, 2216–2217.
Zhang X, Liu S, Takano T. 2008. Two cysteine proteinase inhibitors from Arabidopsis thaliana, AtCYSa and AtCYSb, increasing the salt, drought, oxidation and cold tolerance. Plant Molecular Biology, 68, 131–143.
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