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Journal of Integrative Agriculture  2018, Vol. 17 Issue (12): 2734-2744    DOI: 10.1016/S2095-3119(18)62105-7
Special Issue: 线虫合辑Nematology
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Chemical mutagenesis and soybean mutants potential for identification of novel genes conferring resistance to soybean cyst nematode
GE Feng-yong1, ZHENG Na1, ZHANG Liu-ping1, HUANG Wen-kun1, PENG De-liang1, LIU Shi-ming1, 2
1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China
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The resistance of soybean (Glycine max (L.) Merr.) to soybean cyst nematode (SCN, Heterodera glycines Ichinohe), which is a devastating pathogen in soybean production and causes a large quantity of annual yield loss worldwide, can shift during the long-term interaction and domestication.  It is vital to identify more new resistance genetic sources for identification of novel genes underlying resistance to SCN for management of this pathogen.  In the present study, first, two ethane methylsulfonate-mutagenesis soybean M2 populations of PI 437654, which shows a broad resistance to almost all of SCN races, and Zhonghuang 13, which is a soybean cultivar in China conferring strong resistance to lodging, were developed.  Many types of morphological phenotypes such as four- and five-leaflet leaves were observed from these two soybean M2 populations.  Second, 13 mutants were identified and confirmed to exhibit alteration of resistance to SCN race 4 through the forward genetic screening of 400 mutants of the PI 437654 M2 population, the rate of mutants with alteration of SCN-infection phenotype is 3.25%.  Third, these identified mutants were further verified not to show any changes in the genomic sequences of the three known SCN-resistant genes, GmSHMT08, GmSNAP18 and GmSANP11, compared to the wild-type soybean; and all of them were still resistant to SCN race 3 similar to the wild-type soybean.  Taken together, we can conclude that the 13 mutants identified in the present study carry the mutations of the new gene(s) which contribute(s) to the resistance to SCN race 4 in PI 437654 and can be potentially used as the genetic soybean sources to further identify the novel SCN-resistant gene(s).   
Keywords:  soybean        ethane methylsulfonate-mutagenesis populations        mutants       soybean cyst nematode race 4        resistance  
Received: 23 June 2018   Accepted:
Fund: This work was financially supported by the Innovation Program and Youth Elite Program of Chinese Academy of Agricultural Sciences and the Special Fund for Agro-scientific Research in the Public Interest of China (201503114).
Corresponding Authors:  Correspondence LIU Shi-ming, E-mail:   
About author:  Ge Feng-yong, E-mail:;

Cite this article: 

GE Feng-yong, ZHENG Na, ZHANG Liu-ping, HUANG Wen-kun, PENG De-liang, LIU Shi-ming. 2018. Chemical mutagenesis and soybean mutants potential for identification of novel genes conferring resistance to soybean cyst nematode. Journal of Integrative Agriculture, 17(12): 2734-2744.

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