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Journal of Integrative Agriculture  2018, Vol. 17 Issue (12): 2724-2733    DOI: 10.1016/S2095-3119(18)61971-9
Special Issue: 线虫合辑Nematology
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Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China
TIAN Zhong-ling1, Munawar Maria1, Eda Marie Barsalote1, Pablo Castillo2, ZHENG Jing-wu1, 3
1 Laboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2 Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, Córdoba 14004, Spain
3 Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, P.R.China
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The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice.  In China, it was first reported from Hainan Province, and later from several other provinces.  In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer (ITS) and cytochrome c oxidase subunit II (coxII)-16S rRNA genes and scanning electron microscopy (SEM) observations of males and the second-stage juveniles.  Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided.  The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description.  This is the first report of M. graminicola from Zhejiang.  Phylogenetic studies based on coxII suggest that all the Chinese populations belong to Type B.  This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China. 
Keywords:  Meloidogyne graminicola        morphology        morphometric        molecular        rice        rDNA        cytochrome c oxidase subunit II (coxII)        China  
Received: 16 January 2018   Accepted:
Fund: This research was supported by the Special Fund for Agro-scientific Research in the Public Interest in China (201503114).
Corresponding Authors:  Correspondence ZHENG Jing-wu, Tel/Fax: +86-571-88982580, E-mail:   
About author:  TIAN Zhong-ling, E-mail:;

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TIAN Zhong-ling, Munawar Maria, Eda Marie Barsalote, Pablo Castillo, ZHENG Jing-wu. 2018. Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China. Journal of Integrative Agriculture, 17(12): 2724-2733.

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