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Special Issue:
植物抗病遗传合辑Plant Disease-resistance Genetics
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| Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| LIU Tai-guo*, GE Run-jing*, MA Yu-tong, LIU Bo, GAO Li, CHEN Wan-quan |
| State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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摘要 Received 10 October, 2017 Accepted 9 February, 2018
Abstract Puccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population structure was analyzed using four presumably neutral partial sequence markers such as elongation factor-1α (EF-1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB) and the second largest RNA polymerase subunit (RPB2). The phenotypic diversity of Yunnan and Sichuan populations was higher than that of Gansu and Henan populations. The four populations were separated into two clusters based on the pathogenic data. A total of 12 single nucleotide polymorphisms (SNPs) and 32 haplotypes were identified among the four sequences. The 32 haplotypes were divided into two clusters in a neighbor-joining tree. Bayesian analyses also identified two clusters. Pairwise Fst between populations in different regions were significantly different (P<0.05). Analysis of molecular variance (AMOVA) indicated that 68% of the total genetic variation was within populations.
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Received: 10 October 2017
Accepted:
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| Fund: The financial supports by the National Natural Science Foundation of China (31671967), the National Key Research and Development Program from the Ministry of Science and Technology, China (2016YFD0300705), the National GMO New Variety Breeding Project, China (2014ZX0801101B) and the earmarked fund for China Agriculture Research System (CARS-3) were gratefully acknowledged. |
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Corresponding Authors:
Correspondence LIU Tai-guo, Tel: +86-10-62815618, Fax: +86-10-62815909, E-mail: tgliu@ippcaas.cn; CHEN Wan-quan, E-mail: wqchen@ippcaas.cn
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| About author: * These authors contributed equally to this study. |
Cite this article:
LIU Tai-guo, GE Run-jing, MA Yu-tong, LIU Bo, GAO Li, CHEN Wan-quan.
2018.
Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences. Journal of Integrative Agriculture, 17(08): 1779-1789.
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