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Journal of Integrative Agriculture  2018, Vol. 17 Issue (05): 965-974    DOI: 10.1016/S2095-3119(17)61719-2
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Molecular mechanisms controlling seed set in cereal crop species under stress and non-stress conditions
LI Hui-yong1, 2, Thomas Lübberstedt2
1 Cereal Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China
2 Department of Agronomy, Iowa State University, Ames, IA 50010, USA
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Abstract  Maximizing seed yield is the ultimate breeding goal in important cereal crop species.  Seed set is a key developmental stage in the process of seed formation, which determines grain number, seed mass, and realized yield potential, and can be severely affected by abiotic and biotic stresses.  However, seed set can also be substantially reduced by genetic factors even under optimal fertilization conditions.  The underlying molecular genetic mechanisms are still obscure.  In this review, we elucidate the process of seed set of cereal crop species in detail, including development of floral structures, formation of viable gametes, double fertilization, seed development, and abortion.  We discuss how genetic and non-genetic factors affect seed set in different development stages.  Finally, we will propose novel strategies to study genetic mechanisms controlling seed set and exploit genetic resources to improve seed set in cereal crop species.
Keywords:  maize       seed set        molecular mechanism  
Received: 02 May 2017   Accepted: 04 May 2018

Authors would like to thank the National Key Research and Development Program of China (2016YFD100103), the Major Science and Technology Projects in Henan Province, China (161100110500, 151100111000), the Science Foundation for the Excellent Youth Scholars of Henan Academy of Agricultural Sciences, China (2016YQ04), the International Cooperation Project in Henan Province, China (162102410034), as well as USDA’s National Institute of Food and Agriculture (IOW04314, IOW01018), the RF Baker Center for Plant Breeding and K. J. Frey Chair in Agronomy at Iowa State University for funding this work.

Corresponding Authors:  Correspondence LI Hui-yong, Tel: +86-371-65758930, E-mail:   

Cite this article: 

LI Hui-yong, Thomas Lübberstedt. 2018. Molecular mechanisms controlling seed set in cereal crop species under stress and non-stress conditions. Journal of Integrative Agriculture, 17(05): 965-974.

Achard P, Cheng H, De Grauwe L, Decat J, Schoutteten H, Moritz T, Straeten D, Peng J, Harberd N P. 2006. Integration of plant responses to environmentally activated phytohormonal signals. Science, 311, 91–94.

Albacete A, Ghanem M E, Martínez-Andújar C, Acosta M, Sánchez-Bravo J, Martínez V, Lutts S, Dodd I C, Pérez-Alfocea F. 2008. Hormonal changes in relation to biomass partitioning and shoot growth impairment in salinized tomato (Solanum lycopersicum L.) plants. Journal of Experimental Botany, 59, 4119–4131.

Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles E R, Qian Q, Kitano H, Matsuoka M. 2005. Cytokinin oxidase regulates rice grain production. Science, 309, 741–745.

Aylor D E. 2003. Rate of dehydration of corn (Zea mays L.) pollen in the air. Journal of Experimental Botany, 54, 2307–2312.

Barcaccia G, Albertini E. 2013. Apomixis in plant reproduction: A novel perspective on an old dilemma. Plant Reproduction, 26, 159–179.

Barnabás B, Jäger K, Fehér A. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell & Environment, 31, 11–38.

Bashaw E C. 1980. Apomixis and its application in crop improvement. Hybridization of Crop Plants. [2017-03-15].

Berger S, Sinha A K, Roitsch T. 2007. Plant physiology meets phytopathology: Plant primary metabolism and plant-pathogen interactions. Journal of Experimental Botany, 58, 4019–4026.

Bernier G, Perilleux C. 2005. A physiological overview of the genetics of flowering time control. Plant Biotechnology Journal, 3, 3–16.

Bilgin D D, Zavala J A, Zhu J, Clough S J, Ort D R, Delucia E H.  2010. Biotic stress globally downregulates photosynthesis genes. Plant, Cell & Environment, 33, 1597–1613.

Borrás L, Maddonni G A, Otegui M E. 2003. Leaf senescence in maize hybrids: Plant population, row spacing and kernel set effects. Field Crops Research, 82, 13–26.

Bortiri E, Hake S. 2007. Flowering and determinacy in maize. Journal of Experimental Botany, 58, 909–916.

Boss P K, Bastow R M, Mylne J S, Dean C. 2004. Multiple pathways in the decision to flower: Enabling, promoting, and resetting. The Plant Cell, 16(Suppl.), S18–S31.

Boyer J S, McLaughlin J E. 2007. Functional reversion to identify controlling genes in multigenic responses: Analysis of floral abortion. Journal of Experimental Botany, 58, 267–277.

Brekke B, Edwards J, Knapp A. 2011. Selection and adaptation to high plant density in the Iowa stiff stalk synthetic maize (Zea mays L.) population: II. Plant morphology. Crop Science, 51, 2344–2351.

Buitink J, Wakers B, Hoekstra F A, Crane J. 1996. Storage behavior of Typha latifolia pollenat low water content: Interpretation on the basis of water activity and glass concept. Physiologia Plantarum, 103, 145–153.

Cai G, Faleri C, Del Casino C, Emons A M C, Cresti M. 2011. Distribution of callose synthase, cellulose synthase, and sucrose synthase in tobacco pollen tube is controlled in dissimilar ways by actin filaments and microtubules. Plant Physiology, 155, 1169–1190.

Do Canto J, Studer B, Lubberstedt T. 2016. Overcoming self-incompatibility in grasses: A pathway to hybrid breeding. Theoretical and Applied Genetics, 129, 1815–1829.

Carcova J, Uribelarrea M, Otegui M, Westgate M E. 2000. Synchronous pollination within and between ears improves kernel set in maize. Crop Science, 40, 1056–1061.

Cassman K G, Dobermann A, Walters D T, Yang H. 2003. Meeting cereal demand while protecting natural resources and improving environmental quality. Annual Review of Environment and Resources, 28, 315–358.

Chaudhury A M, Ming L, Miller C, Craig S, Dennis E S, Peacock W J. 1997. Fertilization-independent seed development in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 94, 4223–4228.

Chaves M M, Maroco J P, Pereira J S. 2003. Understanding plant responses to drought  -  from genes to the whole plant. Functional Plant Biology, 30, 239–264.

Craufurd P Q, Peacock J M. 1993. Effect of heat and drought stress on sorghum (Sorghum bicolor). II. Grain yield. Experimental Agriculture, 29, 77–86.

Danilevskaya O N, Meng X, Selinger D A, Deschamps S, Hermon P, Vansant G, Gupta R, Ananiev E V, Muszynski M G. 2008. Involvement of the MADS-box gene ZMM4 in floral induction and inflorescence development in maize. Plant Physiology, 147, 2054–2069.

Danyluk J, Kane N A, Breton G, Limin A E, Fowler D B, Sarhan F. 2003. TaVRT-1, a putative transcription factor associated with vegetative to reproductive transition in cereals. Plant Physiology, 132, 1849–1860.

Van Dijk P J, Rigola D, Schauer S E. 2016. Plant breeding: Surprisingly, less sex is better. Current Biology, 26, R122–R124.

Dilbirligi M, Erayman M, Campbell B T, Randhawa H S, Baenziger P S, Dweikat I, Gill K S. 2006. High-density mapping and comparative analysis of agronomically important traits on wheat chromosome 3A. Genomics, 88, 74–87.

Dong Z, Danilevskaya O, Abadie T, Messina C, Coles N, Cooper M. 2012. A gene regulatory network model for floral transition of the shoot apex in maize and its dynamic modeling. PLoS ONE, 7, e43450.

Duvick D N. 1997. What is yield? In: Proceedings of a Symposium. El Batan, Mexico. pp. 25–29. 

Ekanayake I J, Steponkus P L, de Datta S K. 1990. Sensitivity of pollination to water deficits at antheis in upland rice. Crop Science, 30, 310–315.

FAO (Food and Agriculture Organization of the United Nations).  2015. FAOSTAT. [2017-03-15].

Fasoula V A, Fasoula D A. 2002. Principles underlying genetic improvement for high and stable crop yield potential. Field Crops Research, 75, 191–209.

Ferris R, Ellis R H, Wheeler T R, Hadley P. 1998. Effect of high temperature stress at anthesis on grain yield and biomass of field-grown crops of wheat. Annals of Botany, 82, 631–639.

Fornara F, Pa?enicová L, Falasca G, Pelucchi N, Masiero S, Ciannamea S, Lopez-Dee Z, Altamura M M, Colombo L, Kater M M. 2004. Functional characterization of OsMADS18, a member of the AP1/SQUA subfamily of MADS box genes. Plant Physiology, 135, 2207–2219.

Fischer R A. 1985. Number of kernels in wheat crops and the influence of solar radiation and temperature. The Journal of Agricultural Science, 105, 447–461.

Fuad-hassan A, Tardieu F, Turc O. 2008. Drought-induced changes in anthesis-silking interval are related to silk expansion: A spatio-temporal growth analysis in maize plants subjected to soil water deficit. Plant, Cell & Environment, 31, 1349–1360.

Grassini P, Thorbum J, Burr C, Cassman K G. 2011. High yield irrigated maize in the Western US Corn Belt: I. On-farm yield, yield potential, and impact of agronomic practices. Field Crops Research, 120, 142–150.

Grossniklaus U, Vielle-Calzada J P, Hoeppner M A, Gagliano W B. 1998. Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis. Science, 280, 446–450.

Groszmann M, Gonzalez-Bayon R, Lyons R L, Greaves I K, Kazan K, Peacock W J, Dennis E S. 2015. Hormone-regulated defense and stress response networks contribute to heterosis in Arabidopsis F1 hybrids. Proceedings of the National Academy of Sciences of the United States of America, 112, E6397–E6406.

Ha S, Vankova R, Yamaguchi-Shinozaki K, Shinozaki K, Tran L S P. 2012. Cytokinins: metabolism and function in plant adaptation to environmental stresses. Trends in Plant Science, 17, 172–179.

Hammer G L, Dong Z, McLean G, Doherty A, Messina C, Schussler J, Zinselmeier C, Paszkiewicz S, Cooper M. 2009. Can changes in canopy and/or root system architecture explain historical maize yield trends in the US Corn Belt? Crop Science, 49, 299–312.

Hanna W, Roche D, Ozias-Akins P. 1998. Apomixis in crop improvement: Traditional and molecular approaches. In: Advances in Hybrid Rice Technology: Proceedings of the 3rd International Symposium on Hybrid Rice, 14–16 November 1996. International Rice Research Institute, Hyderabad, India. p. 283.

Hashemi A M, Herbert S J, Putnam D H. 2005. Yield response of corn to crowding stress. Agronomy Journal, 97, 839–846.

Hoekstra F A, Crowe L M, Crowe J H. 1989. Differential dessiccation sensitivity of corn and Pennisetum pollen linked to sucrose content. Plant, Cell & Environment, 12, 83–91.

Holmes M G, Smith H. 1997. The function of phytochrome in the natural environment - IV. Light quality and plant development. Photochemistry and Photobiology, 25, 551–557.

Huang X, Qian Q, Liu Z, Sun H, He S, Luo D, Xia G M, Chu C C, Li J Y, Fu X D. 2009. Natural variation at the DEP1 locus enhances grain yield in rice. Nature Genetics, 41, 494–497.

Iqbal N, Nazar R, Khan M I R, Masood A, Khan N A. 2011. Role of gibberellins in regulation of source-sink relations under optimal and limiting environmental conditions. Current Science, 100, 998–1007.

Itoh H, Nonoue Y, Yano M, Izawa T. 2010. A pair of floral regulators sets critical day length for Hd3a florigen expression in rice. Nature Genetics, 42, 635–638.

Izawa T, Takahashi Y, Yano M. 2003. Comparative biology comes into bloom: Genomic and genetic comparison of flowering pathways in rice and Arabidopsis. Current Opinion in Plant Biology, 6, 113–120.

Kandemir N, Saygili I. 2015. Apomixis: New horizons in plant breeding. Turkish Journal of Agriculture and Forestry, 39, 549–556.

Kebrom T H, Brutnell T P. 2007. The molecular analysis of the shade avoidance syndrome in the grasses has begun. Journal of Experimental Botany, 58, 3079–3089.

Koonjul P K, Minhas J S, Nunes C, Sheoran I S, Saini H S. 2005. Selective transcriptional down-regulation of anther invertases precedes the failure of pollen development in water-stressed wheat. Journal of Experimental Botany, 56, 179–190.

Köhler C, Makarevich G. 2006. Epigenetic mechanisms governing seed development in plants. EMBO Reports, 7, 1223–1227.

Kudlicka K, Brown Jr R M. 1997. Cellulose and callose biosynthesis in higher plants (I. Solubilization and separation of (1-> 3)-and (1-> 4)-[beta]-glucan synthase activities from mung bean). Plant Physiology, 115, 643–656.

Lagercrantz U. 2009. At the end of the day: A common molecular mechanism for photoperiod responses in plants? Journal of Experimental Botany, 60, 2501–2515.

Lashkari M, Madani H, Ardakani M R, Golzardi F, Zargari K. 2011. Effect of plant density on yield and yield components of different corn (Zea mays L.) hybrids. American-Eurasian Journal of Agricultural & Environmental Sciences, 10, 450–457.

Li X, Paech N, Nield J, Hayman D, Langridge P. 1997. Self-incompatibility in the grasses: Evolutionary relationship of the S gene from Phalaris coerulescens to homologous sequences in other grasses. Plant Molecular Biology, 34, 223–232.

Li Y, Ma X, Wang T, Li Y, Liu C, Liu Z Z, Sun B C, Shi Y S, Song Y C, Carlone M, Bubeck D, Bhardwaj H, Whitaker D, Wilson W, Jones E, Wright K, Sun S K, Niebur W, Smith S. 2011. Increasing maize productivity in China by planting hybrids with germplasm that responds favorably to higher planting densities. Crop Science, 51, 2391–2400.

Loukoianov A, Yan L, Blechl A, Sanchez A, Dubcovsky J. 2005. Regulation of VRN-1 vernalization genes in normal and transgenic polyploid wheat. Plant Physiology, 138, 2364–2373.

Mahalakshmi V, Bidinger F R. 1985. Water stress and time of floral initiation in pearl millet. The Journal of Agricultural Science, 105, 437–445.

Matsui T, Omasa K.  2002. Rice (Oryza sativa L.) cultivars tolerant to high temperature at flowering: Anther characteristics. Annals of Botany, 89, 683–687.

McLaughlin J E, Boyer J S. 2004. Sugar-responsive gene expression, invertase activity, and senescence in aborting maize ovaries at low water potentials. Annals of Botany, 94, 675–689.

Miura K, Ikeda M, Matsubara A, Song X J, Ito M, Asano K, Matsuoka M, Kitano H, Ashikari M. 2010. OsSPL14 promotes panicle branching and higher grain productivity in rice. Nature Genetics, 42, 545–549.

Monneveux P, Sanchez C, Beck D, Edmeades G O. 2006. Drought tolerance improvement in tropical maize source populations. Crop Science, 46, 180–191.

Moore G, Foote T, Helentjaris T, Devos K, Kurata N, Gale M. 1995. Was there a single ancestral cereal chromosome? Trends in Genetics, 11, 81–82.

Morgan J. 1980. Possible role of abscisic acid in reducing seed set in water-stressed wheat plants. Nature, 285, 655–657.

Morgan J, King R. 1984. Association between loss of leaf turgor, abscisic acid levels and seed set in two wheat cultivars. Functional Plant Biology, 11, 143–150.

Murai K, Miyamae M, Kato H, Takumi S, Ogihara Y. 2003. WAP1, a wheat APETALA1 homolog, plays a central role in the phase transition from vegetative to reproductive growth. Plant and Cell Physiology, 44, 1255–1265.

Nabity P D, Zavala J A, DeLucia E H. 2009. Indirect suppression of photosynthesis on individual leaves by arthropod herbivory. Annals of Botany, 103, 655–663.

Naik P K, Mohapatra P K. 1999. Ethylene inhibitors promote male gametophyte survival in rice. Plant Growth Regulation, 28, 29–39.

Nishiyama R Y, Watanabe Y, Fujita Y, Le D T, Kojima M, Werner T, Vankova R, Yamaguchi-Shinozaki K, Shinozaki K, Kakimoto T, Sakakibara H, Schmülling T, Tran L P. 2011. Analysis of cytokinin mutants and regulation of cytokinin metabolic genes reveals important regulatory roles of cytokinins in drought, salt and abscisic acid responses, and abscisic acid biosynthesis. The Plant Cell, 23, 2169–2183.

Ohad N I R, Margossian L, Hsu Y C, Williams C, Repetti P, Fischer R L.1996. A mutation that allows endosperm development without fertilization. Proceedings of the National Academy of Sciences of the United States of America, 93, 5319–5324.

Oliver S N, Dennis E S, Dolferus R. 2007. ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice. Plant and Cell Physiology, 48, 1319–1330.

Ozias-Akins A, Connor J A. 2015. Gene for Induction of Parthenogenesis, a Component of Apomictic Reproduction Patent Application. U.S. Patent, Application No. WO/2015/061355. 2014-10-21.

Pacini E. 1996. Types and meaning of pollen carbohydrate reserves. Sexual Plant Reproduction, 9, 362–366.

Parish R W, Phan H A, Iacuone S, Li S F. 2012. Tapetal development and abiotic stress: A centre of vulnerability. Functional Plant Biology, 39, 553–559.

Pierik R, Sasidharan R, Voesenek L A. 2007. Growth control by ethylene: Adjusting phenotypes to the environment. Journal of Plant Growth Regulation, 26, 188–200.

Pimentel D. 1991. Diversification of biological control strategies in agriculture. Crop Protection, 10, 243–253.

Prabha K, Sood R, Gupta S C. 1982. High temperature-induced inactivation of sporophytic self-incompatibility in ipomoea fistulosa. New Phytologist, 92, 115–122.

Prasad P V V, Boote K J, Allen L H, Sheehy J E, Thomas J M G. 2006. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crops Research, 95, 398–411.

Roitsch T, González M C. 2004. Function and regulation of plant invertases: sweet sensations. Trends in Plant Science, 9, 606–613.

Rosegrant M W, Cline S A. 2003. Global food security: Challenges and policies. Science, 302, 1917–1919.

Rossini M A, Maddonni G A, Otegui M E. 2011. Inter-plant competition for resources in maize crops grown under contrasting nitrogen supply and density: Variability in plant and ear growth. Field Crops Research, 121, 373–380.

Ruan Y L, Jin Y, Yang Y J, Li G J, John B. 2010. Sugar input, metabolism, and signaling mediated by invertase: Roles in development, yield potential, and response to drought and heat. Molecular Plant, 3, 942–955.

Ruan Y L, Patrick J W, Bouzayen M, Osorio S, Fernie A R. 2012. Molecular regulation of seed and fruit set. Trends in Plant Science, 17, 656–665.

Saini H S, Aspinall D. 1982. Abnormal sporogenesis in wheat (Triticum aestivum L.) induced by short periods of high temperature. Annals of Botany, 49, 835–846.

Salse J, Piégu B, Cooke R, Delseny M. 2004. New in silico insight into the synteny between rice (Oryza sativa L.) and maize (Zea mays L.) highlights reshuffling and identifies new duplications in the rice genome. The Plant Journal, 38, 396–409.

Sangoi L, Gracietti M A, Rampazzo C, Bianchetti P. 2002. Response of Brazilian maize hybrids from different eras to changes in plant density. Field Crops Research, 79, 39–51.

Satoh-Nagasawa N, Nagasawa N, Malcomber S, Sakai H, Jackson D. 2006. A trehalose metabolic enzyme controls inflorescence architecture in maize. Nature, 441, 227–230.

Schoper J B, Lambert R J, Vasilas B L, Westgate M E. 1987.Plant factors controlling seed set in maize - The influence of silk, pollen, and ear-leaf water status and tassel heat-treatment at pollination. Plant Physiology, 83, 121–125.

Schussler J R, Westgate M E. 1991. Maize kernel set at low water potential: I. Sensitivity to reduced assimilates during early kernel growth. Crop Science, 31, 1189–1195.

Smith H. 1995. Physiological and ecological function within the phytochrome family. Annual Review of Plant Biology, 46, 289–315.

Smith H M, Samach A. 2013. Constraints to obtaining consistent annual yields in perennial tree crops. I: Heavy fruit load dominates over vegetative growth. Plant Science, 207, 158–167.

Song Y, Ito S, Imaizumi T. 2010. Similarities in the circadian clock and photoperiodism in plants. Current Opinion in Plant Biology, 13, 594–603. 

Sreenivasulu N, Schnurbusch T. 2012. A genetic playground for enhancing grain number in cereals. Trends in Plant Science, 17, 91–101.

Suwa R, Hakata H, Hara H, El-Shemy H A, Adu-Gyamfi J J, Nguyen N T, Kanai S, Lightfoot D A, Mohapatra P K, Fujita K. 2010. High temperature effects on photosynthate partitioning and sugar metabolism during ear expansion in maize (Zea mays L.) genotypes. Plant Physiology and Biochemistry, 48, 124–130.

Suzuki N, Rivero R M, Shulaev V, Blumwald E, Mittler R. 2014. Abiotic and biotic stress combinations. New Phytologist, 203, 32–43.

Szitty G, Silhavy D, Molnár A, Havelda Z, Lovas Á, Lakatos L, Bánfalvi Z, Burgyá J. 2003. Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. The EMBO Journal, 22, 633–640.

Tester M, Bacic A. 2005. Abiotic stress tolerance in grasses. From model plants to crop plants. Plant Physiology, 137, 791–793.

Tian D, Traw M B, Chen J Q, Kreitman M, Bergelson J. 2003. Fitness costs of R-gene-mediated resistance in Arabidopsis thaliana. Nature, 423, 74–77.

Tokatlidis I S, Koutroubas S D. 2004. A review of maize hybrids’ dependence on high plant populations and its implications for crop yield stability. Field Crops Research, 88, 103–114.

Tollenaar M, Lee E A. 2002. Yield potential, yield stability and stress tolerance in maize. Field Crops Research, 75, 161–169.

Vollbrecht E, Springer P S, Goh L, Buckler I V E S, Martienssen R. 2005. Architecture of floral branch systems in maize and related grasses. Nature, 436, 1119–1126.

Weber H, Borisjuk L, Wobus U. 2005. Molecular physiology of legume seed development. Annual Review of Plant Biology, 56, 253–279.

Wellmer F, Riechmann J L. 2010. Gene networks controlling the initiation of flower development. Trends in Genetics, 26, 519–527.

Westgate M E. 1994. Water status and development of the maize endosperm and embryo during drought. Crop Science, 34, 76–83.

Westgate M E, Passioura J B, Munns R. 1996. Water status and ABA content of floral organs in drought-stressed wheat. Functional Plant Biology, 23, 763–772.

Wilkins P W, Thorogood D. 1992. Breakdown of self-incompatibility in perennial ryegrass at high temperature and its uses in breeding. Euphytica, 64, 65–69.

Winkel T, Renno J F, Payne W A. 1997. Effect of the timing of water deficit on growth, phenology and yield of pearl millet (Pennisetum glaucum (L.) R. Br.) grown in Sahelian conditions. Journal of Experimental Botany, 48, 1001–1009.

Wopereis M C S, Kropff M J, Maligaya A R, Tuong T P. 1996. Drought-stress responses of two lowland rice cultivars to soil water status. Field Crops Research, 46, 21–39.

Yang J, Zhang J, Wang Z, Xu G, Zhu Q. 2004. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiology, 135, 1621–1629.

Yano M, Kojima S, Takahashi Y, Lin H, Sasaki T. 2001. Genetic control of flowering time in rice, a short-day plant. Plant Physiology, 127, 1425–1429.

Zalabák D, Pospíšilová H, Šmehilová M, Mrízová K, Frébort I, Galuszka P. 2013. Genetic engineering of cytokinin metabolism: Prospective way to improve agricultural traits of crop plants. Biotechnology Advances, 31, 97–117.

Zinn K E, Tunc-Ozdemir M, Harper J F. 2010. Temperature stress and plant sexual reproduction: Uncovering the weakest links. Journal of Experimental Botany, 61, 1959–1968.

Zou J, Rodriguez-Zas S, Aldea M, Li M, Zhu J, Gonzalez D O, Vodkin L O, DeLucia E, Clough S J. 2005. Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis. Molecular Plant-Microbe Interactions, 18, 1161–1174.
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