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Journal of Integrative Agriculture  2017, Vol. 16 Issue (10): 2168-2176    DOI: 10.1016/S2095-3119(16)61570-8
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Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+ uptake in Saccharomyces cerevisiae
PARK Sung-chul1*, YU Yi-cheng2*, KOU Meng1, YAN Hui1, TANG Wei1, WANG Xin1, LIU Ya-ju1, ZHANG Yun-gang1, KWAK Sang-soo3, MA Dai-fu1, SUN Jian2, LI Qiang1, 2
1 Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China
2 Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China
3 Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305806, Republic of Korea
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Abstract  Soil salinity causes the negative effects on the growth and yield of crops. In this study, two sweet potato (Ipomoea batatas L.) cultivars, Xushu 28 (X-28) and Okinawa 100 (O-100), were examined under 50 and 100 mmol L–1 NaCl stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly, real-time quantitative polymerase chain reaction (RT-qPCR) results indicated that sweet potato high-affinity K+ transporter 1 (IbHKT1) gene expression was highly induced by 50 and 100 mmol L–1 NaCl stress in the stems of X-28 cultivar than in those of O-100 cultivar, but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter, we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium (Na+). Yeast complementation assay showed that IbHKT1 could take up K+ in 50 mmol L–1 K+ medium without the presence of NaCl. Moreover, Na+ uptake significantly increased in yeast overexpressing IbHKT1. These results showed that IbHKT1 transporter could have K+-Na+ symport function in yeast. Therefore, the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other HKT1 transporters in class I. Potentially, IbHKT1 could be used to improve the salt tolerance nature in sweet potato.
Keywords:  IbHKT1        Na+/K+ transporter        salt stress        sweet potato  
Received: 31 August 2016   Accepted: 30 September 2017

This work was supported by the China Agriculture Research System (CARS-10, Sweetpotato), the Agricultural Science and Technology Innovation Program of Jiangsu Province, China (CX(13)2032), and the China-Korea Young Scientist Exchange Program.

Corresponding Authors:  Correspondence LI Qiang, Tel: +86-516-82189203, E-mail:   

Cite this article: 

PARK Sung-chul, YU Yi-cheng, KOU Meng, YAN Hui, TANG Wei, WANG Xin, LIU Ya-ju, ZHANG Yun-gang, KWAK Sang-soo, MA Dai-fu, SUN Jian, LI Qiang. 2017. Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+ uptake in Saccharomyces cerevisiae. Journal of Integrative Agriculture, 16(10): 2168-2176.

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