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Combining gas exchange and chlorophyll a fluorescence measurements to analyze the photosynthetic activity of drip-irrigated cotton under different soil water deficits |
LUO Hong-hai1*, Tsimilli-michael Merope2* , ZHANG Ya-li1, ZHANG Wang-feng1 |
1 Key Laboratory of Oasis Ecology Agriculture, Xinjiang Production and Construction Group, Shihezi University, Shihezi 832003, P.R.China
2 Bioenergetics Laboratory, University of Geneva, Jussy-Geneva CH-1254, Switzerland |
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Abstract Gas exchange and chlorophyll a fluorescence were measured to study the effects of soil water deficit (75, 60 and 45% of field capacity, FC) on the photosynthetic activity of drip-irrigated cotton under field conditions. At light intensities above 1 200 µmol m−2 s−1, leaf net photosynthetic rate (Pn) at 60 and 45% FC was 0.75 and 0.45 times respectively than that of 75% FC. The chlorophyll content, leaf water potential and yield decreased as soil water deficit decreased. Fiber length was significantly lower at 45% FC than at 75% FC. The actual quantum yield of the photosystem II (PSII) primary photochemistry and the photochemical quenching were significantly greater at 60% FC than at 75% FC. The electron transport rate and non-photochemical quenching at 45% FC were 0.91 and 1.29 times than those at 75% FC, respectively. The amplitudes of the K- and L-bands were higher at 45% FC than at 60% FC. As soil water content decreased, active PSII reaction centers per chlorophyll decreased, functional PSII antenna size increased, and energetic connectivity between PSII units decreased. Electron flow from plastoquinol to the PSI end electron acceptors was significantly lower at 45% FC than at 75% FC. Similar to the effect on leaf Pn, water deficit reduced the performance index (PIABS, total) in the dark-adapted state. These results suggest that (i) the effect of mild water deficit on photosystem activity was mainly related to processes between plastoquinol and the PSI end electron acceptors, (ii) PSI end electron acceptors were only affected at moderate water deficit, and (iii) PIABS, total can reliably indicate the effect of water deficit on the energy supply for cotton metabolism.
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Received: 02 June 2015
Accepted:
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Fund: This study was supported by the National Natural Science Foundation of China (31401321 and U1203283), the Pairing Program of Shihezi University with Eminent Scholars in Elite Universities (SDJDZ201510) and the Swiss National Science Foundation (200021-116765). |
Corresponding Authors:
ZHANG Wang-feng, Tel: +86-993-2057326, Fax: +86-993-2057999, E-mail: Zwf_shzu@163.com
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About author: LUO Hong-hai, E-mail: luohonghai79@163.com |
Cite this article:
LUO Hong-hai, Tsimilli-michael Merope, ZHANG Ya-li, ZHANG Wang-feng.
2016.
Combining gas exchange and chlorophyll a fluorescence measurements to analyze the photosynthetic activity of drip-irrigated cotton under different soil water deficits. Journal of Integrative Agriculture, 15(06): 1256-1266.
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Chaves M M. 1991. Effects of water deficits on carbon assimilation. Journal of Experimental Botany, 42, 1–16.Chen S G, Kang Y, Zhang M, Wang X X, Strasser R J, Zhou B, Qiang S. 2015. Differential sensitivity to the potential bioherbicide tenuazonic acid probed by the JIP-test based on fast chlorophyll fluorescence kinetics. Environmental and Experimental Botany, 112, 1–15. Chen Y H, Hsu B D. 1995. Effects of dehydration on the electron transport of Chlorella. An in vivo fluorescence study. Photosynthesis Research, 46, 295–299.Demetriou G, Neonaki C, Navakoudis E, Kotzabasis K. 2007. Salt stress impact on the molecular structure and function of the photosynthetic apparatus-theprotective role of polyamines. Biochimica et Biophysica Acta-Bioenergetics, 1767, 272–280.Demmig-Adams B. 1990. Carotenoids and photoprotection in plant: A role for the xanthophyll zeaxanthin. Biochimica et Biophysica Acta-Bioenergetics, 1020, 1–24.Ennahli S, Earl H J. 2005. Physiological limitations to photosynthetic carbon assimilation in cotton under water stress. Crop Science, 45, 2374–2382. Genty B, Briantais J M, Da Silva J B V. 1987. Effects of drought on primary photosynthetic processes of cotton leaves. Plant Physiology, 83, 360–364. Hsiao T C. 1973. Plant responses to water stress. Annual Review of Plant Biology, 24, 519–570.Jiang C D, Gao H Y, Zou Q. 2001. Enhanced thermal energy dissipation depending on xanthophyll cycle and D1 protein turnover in iron-deficient maize leaves under high irradiance. Photosynthetica, 39, 269–274.Jiao X L. 2014. The effects of non-sufficient irrigation on boll development and fibre quality of cotton. MSc thesis, Xinjiang Agriculture University, China. (in Chinese)Kaiser W M. 1987. Effects of water deficit on photosynthetic capacity. Physiologia Plantarum, 71, 142–149.Kitao M, Lei T T. 2007. Circumvention of over-excitation of PSII by maintaining electron transport rate in leaves of four cotton genotypes developed under long-term drought. Plant Biology, 9, 69–76.Long S P, Humphries S, Falkowski P G. 1994. Photoinhibition of photosynthesis in nature. Annual Review of Plant Biology, 45, 633–662.Oxborough K, Baker N R. 1997. An instrument capable of imaging chlorophyll a fluorescence from intact leaves at very low irradiance and at cellular and subcellular levels of organization. Plant, Cell and Environment, 20, 1473–1483.Quick W P, Chaves M M, Wendler R, David M, Rodrigues M L, Passaharinho J A, Pereira J S, Adcock M D, Leegood R C, Stitt M. 1992. The effect of water stress on photosynthetic carbon metabolism in four species grown under field conditions. Plant, Cell and Environment, 15, 25–35.Raines C A. 2011. Increasing photosynthetic carbon assimilation in C3 plant to improve crop yield: Current and future strategies. Plant Physiology, 155, 36–42.Schansker G, Tóth S Z, Strasser R J. 2005. Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochimica et Biophysica Acta-Bioenergetics, 1706, 250–261.Schreiber U. 2004. Pulse-amplitude modulation (PAM) fluorometry and saturation pulse method: An overview. In: Yunus M, Pathre U, Mohanty P, eds., Probing Photosynthesis: Mechanism, Regulation and Adaptation. Taylor & Francis, London. pp. 279–319. Stirbet A, Govindjee. 2011. On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: Basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology (B-Biology), 104, 236–257.Strasser B J, Strasser R J. 1995. Measuring fast fluorescence transients to address environmental questions: The JIP -test. In: Mathis P, ed., Photosynthesis: From Light to Biosphere. Kluwer Academic Publishers, Dodrecht. pp. 977–980.Strasser R J, Tsimilli-Michael M, Dangre D, Rai M. 2007. Biophysical phenomics reveals functional building blocks of plants systems biology: A case study for the evaluation of the impact of mycorrhization with piriformospora indica. In: Varma A, Oelmüler R, eds., Advanced Techniques in Soil Microbiology, Soil Biology. Springer-Verlag, Berlin Heidelberg. pp. 319–341.Strasser R J, Tsimilli-Michael M, Srivastava A. 2004. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G C, Govindjee, eds., Chlorophyll a Fluorescence: Asignature of Photosynthesis. Advances in Photosynthesis and Respiration Series. Kluwer Academic Publishers, Rotterdam. pp. 321–362.Strasser R J, Tsimilli-Michael M, Qiang S, Goltsev V. 2010. Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. Biochimica et Biophysica Acta-Bioenergetics, 1797, 1313–1326.Tsimilli-Michael M, Strasser R J. 2013. The energy flux theory 35 years later: Formulations and applications. Photosynthesis Research, 117, 289–320.Zhang Y L, Luo H H, Hu Y Y, Strasser R J, Zhang W F. 2013. Characteristics of photosystem II behavior in cotton (Gossypium hirsutum L.) bract and capsule wall. Journal of Integrative Agriculture, 12, 2056–2064. |
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