|
|
|
|
|
| Hybridization between salt resistant and salt susceptible genotypes of mungbean (Vigna radiata L. Wilczek) and purity testing of the hybrids using SSRs markers |
| Sehrawat Nirmala, Yadav Mukesh, Bhat Kangila Venkataraman, Sairam Raj Kumar, Jaiwal Pawan Kumar |
1、Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India
2、Department of Biotechnology, Maharishi Markandeshwar University, Ambala 133207, India
3、National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
4、Indian Society for Plant Physiology, G-3, NASC Complex, Pusa, New Delhi 110012, India |
|
|
|
摘要 Six cultivated and two wild genotypes of mungbean (Vigna radiata L. Wilczek) possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12% (average 3.08%). Molecular analysis confirmed the hybrids purity. All F1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F2 seeds ranged from 5–8 per cluster. The F2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.
Abstract Six cultivated and two wild genotypes of mungbean (Vigna radiata L. Wilczek) possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12% (average 3.08%). Molecular analysis confirmed the hybrids purity. All F1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F2 seeds ranged from 5–8 per cluster. The F2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.
|
|
Received: 15 February 2015
Accepted:
|
|
Corresponding Authors:
Sehrawat Nirmala, Tel: +91-1731-304148,Fax: +91-1731-274375, E-mail: nirmalasehrawat@gmail.com
E-mail: nirmalasehrawat@gmail.com
|
Cite this article:
Sehrawat Nirmala, Yadav Mukesh, Bhat Kangila Venkataraman, Sairam Raj Kumar, Jaiwal Pawan Kumar.
2016.
Hybridization between salt resistant and salt susceptible genotypes of mungbean (Vigna radiata L. Wilczek) and purity testing of the hybrids using SSRs markers. Journal of Integrative Agriculture, 15(3): 521-527.
|
| Abd-Alla M H, Vuong T D, Harper J E. 1998. Genotypicdifferences in nitrogen fixation response to NaCl stressin intact and grafted soybean. Crop Science, 38, 72-77Abdel-Hamid A M E, Mohamed H I. 2014. The effect of theexogenous gibberellic acid on two salt stressed barleycultivars. European Scientific Journal, 10, 228-245Adinarayanamurty V V, Rao M V B, Satyanarayana A,Subramanyam D. 1993. The crossability of V. mungo andV. radiata with V. trilobata. International Journal of TropicalAgricultural, 11, 209-213Agarwal P K, Shukla P S, Gupta K, Jha B. 2013. Bioengineeringfor salinity tolerance in plants: State of the art. MolecularBiotechnology, 54, 102-123Ahmad F, Gaur P M, Croser J S. 2005. Chickpea (Cicer arietinumL.). In: Singh R J, Jauhar P P, eds., Genetic Resources,Chromosome Engineering and Crop Improvement. vol.1. Grain legumes. CRC Press, Boca Raton, Florida. pp.187-217Ahmad M, Sandhu G R. 1988. Response of legumes to saltstress: Effect on growth and nitrogen status of soybean.Pakistan Journal Agriculture Research, 9, 463-468Ashraf M Y, Sarwar G, Ashraf M, Afaf R, Sattar A. 2002. Salinityinduced changes in α-amylase activity during germinationand early cotton seedling growth. Biologia Plantarum, 45,589-591Asif M, Mehboob-Ur-Rahman, Zafar Y. 2006. Genotypinganalysis of six maize (Zea mays L.) hybrids using DNAfingerprinting technology. Pakistan Journal of Botany, 38,1425-1430Birchler J A, Yao H, Chudalayandi S. 2006. Unraveling thegenetic basis of hybrid vigor. Proceedings of the NationalAcademy of Sciences of the United States of America, 103,12957-12958Boling M, Sander D A, Matlock R S. 1961. Mungbeanhybridization technique. Agronomy Journal, 53, 54-55Chaudhary D, Sainger M, Sahoo L, Jaiwal P K. 2010. Genetictransformation of Vigna species: Current status and futureprospective. In: Proceedings of the 14th InternationalWorkshop on Genetic Resources and ComparativeGenomics of Soybean and Vigna. National Institute ofAgrobiological Sciences (NIAS), Tsukuba, Japan. pp. 1-8Epstein E, Norlyn J O, Rush D W, Kingsbury R W, Kelly D B,Cunningham G A, Wrona A F. 1980. Saline culture of crops.Science, 210, 399-404FAO (Food and Agricultural Organization), PNMS (PlantNutrition Management Service). 2009. How to feed theworld in 2050. [2012-05-25]. http://www.fao.org/wsfs/forum2050/en/Groszmann M, Gonzalez-Bayon R, Greaves I K, Wang L,Huen A K, Peacock W J, Dennis E S. 2014. Intraspecificarabidopsis hybrids show different patterns of heterosisdespite the close relatedness of the parental genomes.Plant Physiology, 166, 265-280Kausar A, Ashraf M Y, Ali I, Niaz M, Abbass Q. 2012.Evaluation of sorghum varieties/lines for salt tolerance usingphysiological indices as screening tool. Pakistan Journal ofBotany, 44, 47-52Khajudparn P, Prajongjai T, Poolsawat O, Tantasawat PA. 2012. Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata). Genetics andMolecular Research, 11, 3329-3338Khan M A, Shirazi M U, Khan M A, Mujtaba S M, Islam E,Mumtaz S, Shereen A, Ansari R U, Ashraf M Y. 2009.Role of proline, K/Na ratio and chlorophyll content in salttolerance of wheat (Triticum aestivum L.). Pakistan Journalof Botany, 41, 633-638Khattak G S S, Ashraf M, Saeed I, Alam B. 2006. A new highyielding mungbean (Vigna radiata (L.) Wilczek) variety“ramzan” for the agro climatic conditions of NWFP. PakistanJournal of Botany, 38, 301-310Kumar B S, Prakash M, Narayanan S, Gokulakrishnan J. 2012.Breeding for salinity tolerance in mungbean. APCBEEProcedia, 4, 30-35Lin X C, Lou Y F, Liu J, Peng J S, Liao G L, Fang W. 2010.Crossbreeding of phyllostachys species (Poaceae) andidentification of their hybrids using ISSR markers. Geneticsand Molecular Research, 9, 398-404Mohammed A H M A. 2007. Physiological aspects of mungbeanplant (Vigna radiata L. Wilczek) in response to salt stressand gibberellic acid treatment. Research Journal ofAgriculture and Biological Sciences, 3, 200-213Nair R M, Schafleitner R, Kenyon L, Srinivasan R, EasdownW, Ebert A, Hanson P. 2012. Genetic improvement ofmungbean. SABRAO Journal of Breeding and Genetics,44, 177-190Pandiyan M, Senthil N, Anitha M, Raveendran M, Sudha M,Latha M, Nagarajan P, Toomoka N, Balasubramanian P.2012. Diversity analysis of Vigna sp. through morphologicalmarkers. Wudpecker Journal of Agricultural Research, 1,335-340Parida A, Das A B. 2005. Salt tolerance and salinity effectson plants. Ecotoxicology and Environmental Safety, 60,324-349Paroda R S, Thomas T A. 1988. Genetic resources ofmungbean (Vigna radiata L. Wilczek) in India. In: Mungbean:Proceedings of the Second International Symposium. 16-20November 1987, Bangkok, Thailand AVRDC, Shanhua,Tainan, Taiwan, China. pp. 19-28Reddy M P, Sarla N, Siddiq E A. 2002. Inter simple sequencerepeat (ISSR) polymorphism and its application in plantbreeding. Euphytica, 128, 9-17Saha P, Chatterjee P, Biswas A K. 2010. NaCl pretreatmentalleviates salt stress by enhancement of antioxidant defenseand osmolyte accumulation in mungbean (Vigna radiataL. Wilczek). Indian Journal of Experimental Biology, 48,593-600Sahoo L, Jaiwal P K. 2008. Asiatic beans 115-132 In: Kole C,Hall T C, eds., A Compen dium of Transgenic Crop Plants.Blackwell Publ, Oxford, UK.Sehrawat N, Bhat K V, Kaga A, Tomooka N, Yadav M, JaiwalP K. 2014a. Development of new gene-specific markersassociated with salt tolerance for mungbean (Vigna radiataL. Wilczek). Spanish Journal of Agricultural Research, 12,732-741Sehrawat N, Bhat K V, Sairam R K, Jaiwal P K. 2013a.Identification of salt resistant wild relatives of mungbean(Vigna radiata (L.) Wilczek). Asian Journal of Plant Scienceand Research, 3, 41-49Sehrawat N, Bhat K V, Sairam R K, Jaiwal P K. 2014b.Screening of mungbean [Vigna radiata (L.) Wilczek]genotypes for salt tolerance. International Journal of Plant,Animal and Environmental Sciences, 4, 36-43Sehrawat N, Bhat K V, Sairam R K, Toomoka N, Kaga A, ShuY, Jaiwal P K 2013b. Diversity analysis and confirmation ofintra-specific hybrids for salt tolerance in mungbean (Vignaradiata L. Wilczek). International Journal of IntegrativeBiology, 14, 65-73Sehrawat N, Yadav M, Bhat K V, Sairam R K, Jaiwal P K. 2014c.Evaluation of mungbean genotypes for salt tolerance atearly seedling growth stage. Biocatalysis and AgriculturalBiotechnology, 3, 108-113Singh T P, Malhotra R S. 1975. Crossing technique in mungbean(Phaseolus aureus Roxb.). Current Science, 44, 64-65Virmani S S, Singh K B, Singh K, Malhotra R S. 1983. Evaluationof mungbean germplasm. Indian Journal of Genetics andPlant Breeding, 43, 54-58Win T, Oo A Z, Hirasawa T, Ookawa T, Yutaka H. 2011.Genetic analysis of Myanmar Vigna species in responsesto salt stress at the seedling stage. African Journal ofBiotechnology, 10, 1615-1624Yohe J M, Poehlman J M. 1972. Genetic variability in themungbean, Vigna radiata (L.) Wilczek. Crop Science, 12,461–464. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
