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Cultured meat from muscle stem cells: A review of challenges and prospects |
Isam T Kadim, Osman Mahgoub, Senan Baqir, Bernard Faye , Roger Purchas |
1、Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat
PC. 123, Sultanate of Oman
2、Department of Biology, College of Science, Sultan Qaboos University, Muscat PC. 123, Sultanate of Oman
3、FAO Consultant, Camel Project, Al-Kharj 11941, Saudi Arabia
4、Institute of Food, Nutrition & Human Health, Massey University, Palmerston North Private Bag 11 222, New Zealand |
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摘要 Growing muscle tissue in culture from animal stem cells to produce meat theoretically eliminates the need to sacrifice animals. So-called “cultured” or “synthetic” or “in vitro” meat could in theory be constructed with different characteristics and be produced faster and more efficiently than traditional meat. The technique to generate cultured muscle tissues from stem cells was described long ago, but has not yet been developed for the commercial production of cultured meat products. The technology is at an early stage and prerequisites of implementation include a reasonably high level of consumer acceptance, and the development of commercially-viable means of large scale production. Recent advancements in tissue culture techniques suggest that production may be economically feasible, provided it has physical properties in terms of colour, flavour, aroma, texture and palatability that are comparable to conventional meat. Although considerable progress has been made during recent years, important issues remain to be resolved, including the characterization of social and ethical constraints, the fine-tuning of culture conditions, and the development of culture media that are cost-effective and free of animal products. Consumer acceptance and confidence in in vitro produced cultured meat might be a significant impediment that hinders the marketing process.
Abstract Growing muscle tissue in culture from animal stem cells to produce meat theoretically eliminates the need to sacrifice animals. So-called “cultured” or “synthetic” or “in vitro” meat could in theory be constructed with different characteristics and be produced faster and more efficiently than traditional meat. The technique to generate cultured muscle tissues from stem cells was described long ago, but has not yet been developed for the commercial production of cultured meat products. The technology is at an early stage and prerequisites of implementation include a reasonably high level of consumer acceptance, and the development of commercially-viable means of large scale production. Recent advancements in tissue culture techniques suggest that production may be economically feasible, provided it has physical properties in terms of colour, flavour, aroma, texture and palatability that are comparable to conventional meat. Although considerable progress has been made during recent years, important issues remain to be resolved, including the characterization of social and ethical constraints, the fine-tuning of culture conditions, and the development of culture media that are cost-effective and free of animal products. Consumer acceptance and confidence in in vitro produced cultured meat might be a significant impediment that hinders the marketing process.
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Received: 24 January 2014
Accepted:
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Corresponding Authors:
Isam T Kadim, E-mail: isam@squ.edu.om
E-mail: isam@squ.edu.om
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Cite this article:
Isam T Kadim, Osman Mahgoub, Senan Baqir, Bernard Faye , Roger Purchas.
2015.
Cultured meat from muscle stem cells: A review of challenges and prospects. Journal of Integrative Agriculture, 14(2): 222-233.
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Aisen P, Enns C, Wessling-Resnick M. 2001. Chemistry andbiology of eukaryotic iron metabolism. International Journalof Biochemistry and Cell Biology, 33, 940-959Bach A D, Stem-Straeter J, Beier J P, Bannasch H, Stark GB. 2003. Engineering of muscle tissue. Clinics in PlasticSurgery, 30, 589-599Baquero-Perez B, Kuchipudi S V, Nelli R K, Chang K C. 2012.A simplified but robust method for the isolation of avian andmammalian muscle satellite cells. BMC Cell Biology, 13, 16.Benjaminson M A, Gilchriest J A, Lorenz M. 2002. In vitro ediblemuscle protein production system (MPPS): Stage 1, fish.Acta Astronautica, 51, 879-889Bhat Z F, Fayaz H. 2011. Prospectus of cultured meatadvancingmeat alternatives. Journal of Food Science &Technology, 48, 125-140Blanton J R, Grand A L, McFarland D C, Robinson J, BidwellC A. 1999. Isolation of two populations of myoblasts fromporcine skeletal muscle. Muscle and Nerve, 22, 43-50Boonen K J, Langelaan M L, Polak R B, van der Schaft DW, Baaijens F P, Post M J. 2010. Effects of a combinedmechanical stimulation protocol: Value for skeletalmuscle tissue engineering. Journal of Biomechanics, 43,1514-1521Campion D R. 1984. The muscle satellite cell: A review.International Review of Cytology, 87, 225-251Canavan H E, Cheng X, Graham D J, Ratner B D, Castner DG. 2005. Cell sheet detachment affects the extracellularmatrix: A surface science study comparing thermal lift offenzymatic and mechanical methods. Journal of BiomedicalMaterials Research, 75A, 1-13Carrier R L, Papadaki M, Rupnick M, Schoen F J, Bursac N,Langer R. 1999. Cardiac tissue engineering: Cell seedingcultivation parameters and tissue construct characterization.Biotechnology and Bioengineering, 64, 580-589Catts O, Zurr I. 2002. Growing semi-living sculptures: The tissueculture project. Leonardo, 35, 365-370CDC (Centre for Disease Control). 2012. CDC estimates offoodborne illness in the United States. [2014-5-13]. http://www.cdc.gov/foodborneburden/2011-foodborne-estimates.htmlChurchill W S. 1932. Thoughts and Adventures. ThorntonButterworth, London.Claeys E, De Smet S, Balcaen A, Raes K, Demeyer D. 2004.Quantification of fresh meat peptides by SDS-page inrelation to ageing time and taste intensity. Meat Science,67, 281-288Coecke S, Balls M, Bowe G, Davis J, Gstraunthaler G, HartungT. 2005. Guidance on good cell culture practice: A reportof the second ECVAM Task Force on good cell culturepractice. Alternatives to Laboratory Animals, 33, 261-287Danoviz M E, Yablonka-Reuveni Z. 2012. Skeletal musclesatellite cells: Background and methods for isolation andanalysis in a primary culture system. Methods in MolecularBiology, 798, 21-52Datar I, Betti M. 2010. Possibilities for an in vitro meatproduction system. Innovative Food Science and EmergingTechnologies, 11, 13-22De Deyne P G. 2000. Formation of sarcomeres in developingmyotubes: role of mechanical stretch and contractileactivation. American Journal of Cell Physiology, 279,C1801-C1811.DeGrazia D. 1996. Taking Animals Seriously: Mental Life andMoral Status. Cambridge University Press, UK.Dennis R G, Kosnik P 2nd. 2000. Excitability and isometriccontractile properties of mammalian skeletal muscleconstructs engineered in vitro, In Vitro Cellular & Developmental Biology Animal, 36, 327-335Dennis R G, Smith B, Philip A, Donnelky K, Baar K. 2009.Bioreactors for guiding muscle tissue growth anddevelopment. Advances in Biochemical Engineering(Biotechnology), 112, 39-79Dodson M V, Mathison B A 1988. Comparison of ovine and ratmuscle-derived satellite cells: Response to insulin. Tissueand Cell, 20, 909-918Dodson M V, McFarland D C, Martin E L, Brannon M A. 1986.Isolation of satellite cells from ovine skeletal muscles.Journal of Tissue Culture Methods, 10, 233-237Dodson M V, Martin E L, Brannon M A, Mathison B A, McFarlandD C. 1987. Optimization of bovine satellite cell derivedmyotube formation cultured. Tissue and Cell, 19, 159-166Dodson M V, Wei S, Duarte M, Du M, Jiang Z, Hausman G J,Bergen W G. 2012. Cell supermarket: Adipose tissue as asource of stem cells. Journal of Genomics, 1, 39-44Doumit M E, Cook D R, Merkel R A. 1993. Fibroblast growthfactor epidermal growth factor insulin-like growth factorand platelet-derived growth factor-BB stimulate proliferateof clonally derived porcine myogenic satellite cells. Journalof Cellular Physiology, 157, 326-332Duque P, Gómez E, Diaz E, Facal N, Hidalgo C, Diez C. 2003.Use of two replacements of serum during bovine embryoculture cultured in vitro. Theriogenology, 59, 889-899Edelman P D, McFarland D C, Mironov V A, Matheny J G. 2005.Commentary: In vitro-cultured meat production. TissueEngineering, 11, 659-662Van Eelen W. 2007. Patent holder Willem van Eelen: ‘In anotherfive years meat will come out of the factory’. In Vitro MeatFoundation operated by Willem van Eelen publishing.[2014-5-10] http://www.invitromeatfoundation.eu/uk/publications.phpVan Eelen W F, van Kooten W J, Westerhof W. 1999. Industrialproduction of meat from cultured cell cultures. Patentdescription. [2014-6-20]. http://www.wipoint/pctdb/en/wojsp?wo=1999031223Fiala N. 2008. Meeting the demand: An estimation of potentialfuture greenhouse gas emissions from meat production.Ecological Economics, 67, 412-419Frerich B, Winter K, Scheller K, Braumann U D. 2011.Comparison of different fabrication techniques for humanadipose tissue engineering in severe combined immunedeficientmice. Artificial Organs, 36, 227-237Froud S J. 1999. The development benefits and disadvantagesof serum-free media. Developments in BiologicalStandardization, 99, 157-166Goodwin J N, Shoulders C W. 2013. The future of meat: Aqualitative analysis of cultured meat media coverage. MeatScience, 95, 445-450Graber S G, Woodworth R C. 1986. Myoglobin expressionin L6 muscle cells. Journal of Biological Chemistry, 261,9150-9154Le Grand F, Rudnicki M A. 2007. Skeletal muscle satellite cellsand adult myogenesis. Current Opinion in Cell Biology,196, 628-633Greger M. 2007. The human/animal interface: Emergence andresurgence of zoonotic infectious diseases. Critical Reviewsin Microbiology, 33, 243-299Grinnell F. 2000. Fibroblast-collagen-matrix contraction: growthfactorsignaling and mechanical loading. Trends in CellBiology, 10, 362-365Hocquette J-F, Gondret F, Beaza E, Medale F, Jurie C, PethwickD W. 2010. Intramuscular fat content in meat-producinganimals: development, genetic, and nutritional control,and identification of putative markers. Animal, 4, 303-319Holden C, Vogel G. 2008. A seismic shift stem cell research.Science, 319, 560-563Hopkins P, Dacey A. 2008. Vegetarian Meat: Could technologysave animals and satisfy meat eaters? Journal ofAgricultural Ethics, 21, 579-596Howard-Borjas P. 1996. Cattle and crisis: the genesis ofunsustainable development in Central America. LandReform, Land Settlement and Cooperatives, (1995),88-116IVMC (The In Vitro Meat Consortium). 2008. Is in vitro meatfuture? [2014-6-16]. http://invitromeat.org/Jun I, Jeong S, Shin H. 2009. The stimulation of myoblastdifferentiation by electrically conductive sub-micron fibers.Biomaterials, 30, 2038-2047Kazama T, Fujie M, Endo T, Kano K. 2008. Mature adipocytederiveddedifferentiated fat cells can transdifferentiate intoskeletal myocytes in vitro. Biochemical and BiophysicalResearch Communications, 377, 780-785Khalifa T, Lymberopoulos A, Theodosiadou E. 2013. Associationof soybean-based extenders with field fertility of stored ram(Ovis aries) semen: A randomized double-blind parallelgroup design. Theriogenology, 79, 517-527Klumpp D, Horch R E, Kneser U, Beier J P. 2010. Engineeringskeletal muscle tissue-new perspectives in vitro andin vivo. Journal of Cellular and Molecular Medicine, 14,2622-2629Kuang S, Rudnicki M A. 2008. The emerging biology of satrllitecells and their therapeutic potential. Trends in MolecularMedicine, 14, 82-91Lam M T, Huang Y C, Birla R K, Takayama S. 2009.Microfeature guided skeletal muscle tissue engineeringfor highly organized three-dimensional free-standingconstructs. Biomaterials, 30, 1150-1155Langelaan M L P, Boonen K J M, Polak R B, Baaijens F P T,Post M J, van der Schaft D W J. 2010. Meet the new meat:tissue engineered skeletal muscle. Trends in Food Science& Technology, 21, 59-66Lawrie R A. 1991. Meat Science. Pergamon Press, Oxford.Marques A, Lourenco H M, Nunes M L, Roseiro C, SantosC, Barrabco A, Rainieri S, Langerholc T, Cencic A. 2011.New tools to assess toxicity, bioaccessibility and uptakeof chemical contaminants in meat and seafood. FoodResearch International, 44, 510-522Mauro A. 1961. Satellite cell of skeletal muscle fibers. Journal of Biophysical and Biochemical Cytology, 9, 493-495McFarland D C, Doumit M E, Minshall R D. 1988. The turkeymyogenic satellite cell: Optimization of cultured proliferationand differentiation. Tissue and Cell, 20, 899-908McFarland D C, Pesall J E, Norberg J M, Dvoracek M A. 1991.Proliferation of the turkey myogenic satellite cell in a serumfreemedium. Comparative Biochemistry and Physiology,99, 163-167Merten O W. 1999. Safety issues of animal productsused in serum-free media. Developments in BiologicalStandardization, 99, 167-180Meyers C, Abrams K. 2010. Feeding the debate: A qualitativeframing analysis of organic food news media coverage.Journal of Applied Communications, 94, 22-36Mouly V, Aamiri A, Bigot A, Cooper R N, Di Donna S, FurlingD, Gidato T, Jaquemin V, Mamchaoui K, Negroni E, PerieS, Renault V, Silva-Barbosa S D, Butler-Brown G S. 2005.The mitotic clock in skeletal muscle regeneration, diseaseand cell mediated therapy. Acta Physiologica Scandivavica,184, 3-15Papanikolaou G, Pantopoulos K. 2005. Iron metabolism andtoxicity. Toxicology and Applied Pharmacology, 202,199-211Post M J. 2012. Review: Cultured meat from stem cells:Challenges and prospects. Meat Science, 92, 297-301Post M J. 2014. Cultured beef: medical technology to producefood. Journal of the Science of Food & Agriculture, 94,1039-1041Powell C P, Smiley B L, Mills J, Vandenburgh H H. 2002.Mechanical stimulation improves tissue engineered humanskeletal muscle. American Journal of Cell Physiology, 283,1557-1565Powell R E, Dodson M V, Cloud J G. 1989. Cultivation anddifferentiation of satellite cells from skeletal muscle of therainbow trout Salmo gairdneri. Journal of ExperimentalZoology, 250, 333-338Radisic M, Marsano A, Maidhof R, Wang Y, Vunjak-NovakovicG. 2008. Cardiac tissue engineering using perfusionbioreactor systems. Nature Protocols, 3, 719-738Roobtouck V D, Ulloa-Montoya F, Verfaillie C M. 2008. Selrenewaland differentiation capacity of young and agedstwm cells. Experimental Cell Research, 314, 1937-1944Rother L. 2003. Relentless foe of the Amazon jungle soybeans.New York Times. Sept 17.Da Silva R M P, Mano J F, Reis R L. 2007. Smart thermoresponsivecoatings and surfaces for tissue engineering: Switching cellmaterialboundaries. Trends in Biotechnology, 25, 577-583Skardal A, Zhang J, Prestwich G D. 2010. Bioprinting vessellikeconstructs using hyaluronan hydrogels crosslinked withtetrahedral polyethylene glycol tetracrylates. Biomaterials,31, 6173-6181Springer N P, Duchin F. 2014. Feeding nine billion peoplesustainably: Conserving land and water through shiftingdiets and changes in technologies. Environmental Science& Technology, 48, 4444-4451Steinfeld H, Gerber P, Wassenaar T, Castel V, Rosales M, DeHaan C. 2006. Livestock’s Long Shadow: EnvironmentalIssues and Options. FAO 978-92-5-195571-7. InformationDivision, Rome, Italy.Stephens N. 2010. In vitro meat: Zombies on the menu?Scripted, 7, 394-401Suman S P, Joseph P. 2013. Myoglobin chemistry and meatcolor. Annual Review of Food Science and Technology,4, 79-99Telugu B P, Ezashi T, Roberts R M. 2010. The promise of stemcell research in pigs and other ungulate species. Stem CellReviews, 6, 31-41Tonsor G T, Olynk N J. 2011. Impacts of animal well-being andwelfare media on meat demand. Journal of AgriculturalEconomics, 62, 13.Tuomisto H L, de Mattos M J T. 2011. Environmental impactsof cultured meat production. Environmental Science andTechnology, 45, 6117-6123Uzel C, Conrad M E. 1998. Absorption of heme iron. Seminarsin Hematology, 35, 27-34van der Valk J, Brunner D, De Smet K, Svenningsen A F,Honegger P, Knudsen L E, Lindl T, Noraberg J, PriceA, Scarino M L, Gstraunthaler G. 2010. Optimization ofchemically defined cell culture media-replacing fetal bovineserum in mammalian in vitro methods. Toxicology In Vitro,24, 1053-1063van der Weele C, Tramper J. 2014. Cultured meat: Every villageits own factory. Trends in Biotechnology, 32, 294-296Vandenburggh H, Shansky J, Del Tatto M, Chromiak J. 1999.Organogenesis of skeletal muscle in tissue culture. Methodsin Molecular Medicine, 18, 217-225Verbeke W, van Wezemael L, de Barcellos M D, Kugler J O,Hocquette J-F, Ueland O, Grunert K G. 2010. Europeanbeef consumers’ interest in a beef eating quality guaranteeinsights from a qualitative study in four EU countries.Appetite, 54, 289-296Verbeke W, Ward R, Viaene J. 2000. Probit analysis of freshmeat consumption in Belgium: Exploring BSE and televisioncommunication impact. Agribusiness, 16, 215-234Verseijden F, Posthumus-van Sluijs S J, van Neck J W, Hofer SO P, Hovius S E R, van Osch G J V M. 2012. Vascularizationof prevascularized and non-prevascularized fibrin basedhuman adipose tissue constructs after implantation in nudemice. Journal of Tissue Engineering and RegenerativeMedicine, 6, 169-178Wang Y X, Rudnicki M A. 2012. Satellite cells, the engines ofmuscle repair. Nature Reviews Molecular Cell Biology, 13,127-133Welin S, Gold J, Berlin J. 2012. In vitro meat: What are themoral issues? In: Kaplan D M, ed., The Philosophy of Food.University of California Press, USA. pp. 292-304Webb S. 2006. Tissue engineers cook up plan for lab-grownmeat. Discover, 27, 43.Wiliamson M. 2003. Space ethics and the protection of thespace environment. Science Policy, 19, 47-52 |
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