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The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo |
BAI Li-jing, JU Hui-ming, MU Yu-lian, YANG Shu-lin, REN Hong-yan, AO Hong, WANG, Chu-duan , LI Kui |
1、State Key Laboratory for Animal Nutrition, Ministry of Agriculture/Key Laboratory of Farm Animal Genetic Resources and Germplasm
Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、College of Animal Science, China Agricultural University, Beijing 100193, P.R.China
3、College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.China |
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摘要 Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.
Abstract Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pcDNA-appA-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89% (16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by the appA gene (P<0.05); these animals also exhibited a strong anti-viral phenotype.
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Received: 05 July 2013
Accepted:
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Fund: This work was supported by the National Transgenic Breeding Project of China (2011ZX08010-003) and the National Natural Science Foundation of China (31272405, 31101683). |
Corresponding Authors:
LI Kui, Tel: +86-10-62813822, E-mail: likui@caas.cn
E-mail: likui@caas.cn
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About author: BAI Li-jing, Tel: +86-10-62833312, E-mail: bailijing0730@163.com |
Cite this article:
BAI Li-jing, JU Hui-ming, MU Yu-lian, YANG Shu-lin, REN Hong-yan, AO Hong, WANG , Chu-duan , LI Kui.
2014.
The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo. Journal of Integrative Agriculture, 13(12): 2741-2749.
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