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CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis |
ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji |
1、The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University,
Hohhot 010021, P.R.China
2、School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.China |
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摘要 Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.
Abstract Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.
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Received: 28 March 2013
Accepted:
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Fund: This work was supported by the Program for Innovative Research of Ministry of Education, China (IRT0833) and the Natural Science Foundation of Inner Mongolia, China (2009ZD05). |
Corresponding Authors:
WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com
E-mail: wuyj1211@163.com
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About author: WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com* These authors contributed equally to this study. |
Cite this article:
ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji.
2014.
CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis. Journal of Integrative Agriculture, 13(8): 1759-1765.
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Behrens J, Lowrick O, Klein-Hitpass L, Birchmeier W. 1991. The E-cadherin promoter: functional analysis of a GC-rich region and an epithelial cell-specific palindromic regulatory element. Proceedings of the National Academy of Sciences of the United States of America, 88, 11495-11499 Borjigin U, Davey R, Hutton K, Herrid M 2011. Expression of promyelocytic leukemia zinc-finger in ovine testis and its application in evaluating the enrichment efficiency of differential plating. Reproduction, Fertility and Development, 22, 733-742 Costa G M J, Avelar G F, Rezende-Neto J V, Campos-Junior P H, Lacerda S M, Andrade B S, Thome R G, Hofmann M C, Franca L R. 2012. Spermatogonial stem cell markers and niche in Equids. PLOS ONE, 7, e44091. da Fernan S T, Frank E A, Anita K D, Parry J G. 2003. N-terminal E-cadherin peptides act as decoy receptors for listeria monocytogenes. Infection and Immunity, 71, 1580-1583 Fujihara M, Kim S M, Minami N, Yamada M, Imai H. 2011. Characterization and in vitro culture of male germ cells from developing bovie testis. Journal of Reproduction and Development, 57, 355-364 Herrid M, Olejnik J, Jackson M, Suchowerska N, Stockwell S, Davey R, Hutton K, Hope S, Hill J R 2009. Irradiation enhances the efficiency of testicular germ cell transplantation in sheep. Biology and Reproduction, 81, 895-905 Hill J R, Dobrinski I. 2006. Male germ cell transplantation in livestock. Reproduction, Fertility and Development, 18, 13-18 Honaramooz A, Yang Y. 2011. Recent advances in application of male germ cell transplantation in farm animals. Veterinary Medicine International, Pii, 657860. Hu T Y, Bai Y, Luo F H, Wu Y J. 2011. Cloning and analysis of sheep CDH1 gene. Biotechnology Bulletin, 9, 96-101 (in Chinese) Lee J O, Kwun H J, Jung J K, Chio K H, Min D S, Jang K L. 2005. Hepatitis B vitus X protein represses E-cadherin expression via activation of DNA methyltransferse1. Oncogene, 24, 6617-6625 Niemann H, Kues W A. 2007. Transgenic farm animals: an update. Reproduction, Fertility and Development, 19, 762-770 Ou S K, Hwang J M, Patterson P H. 1993. A modified method for obtaining large amounts of high titer polyclonal ascites fluid. Journal of Immunological Methods, 165, 75-80 Reding S C, Stepnoski A L, Cloninger E W, Oatley J M. 2010. THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction,139, 893-903Shinohara T, Ishii K, Kanatsu-Shinohara M. 2011. Unstableside population phenotype of mouse spermatogonialstem cells in vitro. The Journal of Reproduction andDevelopment, 57, 288-295Takhashi M, Kanatsu-Shinohara M, Shinohara T. 2010.Generation of genetically modified animals usingspermatogonial stem cells. Development, Growth &Differentiation, 52, 303-310Tokuda M, Kadokawa Y, Kurahashi H, Marunouchi T.2007. CDH1 is a specific marker for undifferentiatedspermatogonia in mouse testes. Biology of Reproduction,76, 130-141Wang F, Liu C, Zhang S S. 2012. Transplantation of goat bonemarrow mesenchymal stem cells (gMSCs) help restorespermatogenesis in endogenous germ cells-depleted mousemodels. Journal of Integrative Agriculture, 12, 483-494Wu Y, Luo F, Bou S. 2005. Prospect of creating transgenicanimals by using spermatogonial transplantation. ChineseScience Bulletin, 50, 2537-2545Zhang Y, Su H, Luo H, Wu S, Liu L, Liu T, Yu B, Wu Y.2011. E-cadherin can be expressed by a small populationof rat undifferentiated spermatogonia in vivo and in vitro.In Vitro Cellular & Developmental Biology, 47, 593-600 |
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