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| CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis |
| ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji |
1、The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University,
Hohhot 010021, P.R.China
2、School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.China |
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摘要 Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.
Abstract Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti- CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.
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Received: 28 March 2013
Accepted:
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| Fund: This work was supported by the Program for Innovative Research of Ministry of Education, China (IRT0833) and the Natural Science Foundation of Inner Mongolia, China (2009ZD05). |
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Corresponding Authors:
WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com
E-mail: wuyj1211@163.com
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| About author: WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623, E-mail: wuyj1211@163.com* These authors contributed equally to this study. |
Cite this article:
ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu , LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji.
2014.
CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis. Journal of Integrative Agriculture, 13(8): 1759-1765.
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