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| Allelopathy of decomposed maize straw products on three soilborn diseases of wheat and the analysis by GC-MS |
| QI Yong-zhi, ZHEN Wen-chao, LI Hai-yan |
| College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China |
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摘要 In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass spectrometry (GC-MS). Culture dish experiments showed that the mycelial growth, sclerotia formation amount and total weight of Rhizoctonia cerealis were promoted at concentrations of 0.03, 0.06 and 0.12 g mL–1 and inhibited at concentration of 0.48 g mL–1 of the decomposed products. No significant effects were found of the product concentrations on average weight of the sclerotia. Mycelial growth of Gaeumannomyces graminis was promoted at almost all concentrations except the highest one. Mycelial growth and spore germination of Bipolaris sorokiniana were significantly inhibited by all concentrations of the decomposed products, with enhanced inhibition effects along with the increased concentrations. The length, number and dry weight of roots together with the root superoxide dismutase activity were promoted by the lowest concentration (0.03 g mL–1), with a synthetic effect index of 0.012, and inhibited by other concentrations. The ion leakage of roots was increased and the root peroxidase activity of roots was lowered by all the treatments. Pot experiments revealed that occurrence of the sharp eyespot was reduced by 0.03 and 0.06 g mL–1 of decomposed products after irrigation. However, the incidence rates and disease indexes were significantly increased by 0.12, 0.24 and 0.48 g mL–1 of decomposed products. The results indicated that incidence rates and disease indexes of the take-all were significantly promoted after being irrigated with the decomposed products, while occurrences of the common rot didn’t change, significantly. GC-MS results showed that the compounds of the decomposed products included organic acids, esters, hydrocarbons, amides and aldehydes, with the proportions 25.26, 24.01, 17.22, 14.39 and 7.73%, respectively. Further analysis investigated that the allelochemicals identified in straw decomposed products contained p-hydroxybenzoic acid (9.21%), dibutyl phthalate (6.94%), 3-phenyl-2-acrylic (5.06%), 4-hydroxy-3,5-dimethoxybenzoic acid (2.26%), hexanoic acid (1.73%), 8-octadecenoic acid (1.06%), 3-(4-hydroxy-3-methoxy-phenyl)-2-propenoic acid (1.04%), 4-hydroxy-3-methoxy-benzoic acid (0.94%) and salicylic acid (0.94%).
Abstract In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass spectrometry (GC-MS). Culture dish experiments showed that the mycelial growth, sclerotia formation amount and total weight of Rhizoctonia cerealis were promoted at concentrations of 0.03, 0.06 and 0.12 g mL–1 and inhibited at concentration of 0.48 g mL–1 of the decomposed products. No significant effects were found of the product concentrations on average weight of the sclerotia. Mycelial growth of Gaeumannomyces graminis was promoted at almost all concentrations except the highest one. Mycelial growth and spore germination of Bipolaris sorokiniana were significantly inhibited by all concentrations of the decomposed products, with enhanced inhibition effects along with the increased concentrations. The length, number and dry weight of roots together with the root superoxide dismutase activity were promoted by the lowest concentration (0.03 g mL–1), with a synthetic effect index of 0.012, and inhibited by other concentrations. The ion leakage of roots was increased and the root peroxidase activity of roots was lowered by all the treatments. Pot experiments revealed that occurrence of the sharp eyespot was reduced by 0.03 and 0.06 g mL–1 of decomposed products after irrigation. However, the incidence rates and disease indexes were significantly increased by 0.12, 0.24 and 0.48 g mL–1 of decomposed products. The results indicated that incidence rates and disease indexes of the take-all were significantly promoted after being irrigated with the decomposed products, while occurrences of the common rot didn’t change, significantly. GC-MS results showed that the compounds of the decomposed products included organic acids, esters, hydrocarbons, amides and aldehydes, with the proportions 25.26, 24.01, 17.22, 14.39 and 7.73%, respectively. Further analysis investigated that the allelochemicals identified in straw decomposed products contained p-hydroxybenzoic acid (9.21%), dibutyl phthalate (6.94%), 3-phenyl-2-acrylic (5.06%), 4-hydroxy-3,5-dimethoxybenzoic acid (2.26%), hexanoic acid (1.73%), 8-octadecenoic acid (1.06%), 3-(4-hydroxy-3-methoxy-phenyl)-2-propenoic acid (1.04%), 4-hydroxy-3-methoxy-benzoic acid (0.94%) and salicylic acid (0.94%).
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Received: 03 January 2014
Accepted:
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| Fund: This research was financially supported by the Key Technologies R&D Program of China during the 12th Five- Year Plan period (2011BAD16B08, 2012BAD04B06 and 2013BAD07B05). |
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Corresponding Authors:
ZHEN Wen-chao, Tel: +86-312-7528158, E-mail: wenchao@hebau.edu.cn
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| About author: QI Yong-zhi, E-mail: qiyongzhi1981@163.com; |
Cite this article:
QI Yong-zhi, ZHEN Wen-chao, LI Hai-yan.
2015.
Allelopathy of decomposed maize straw products on three soilborn diseases of wheat and the analysis by GC-MS. Journal of Integrative Agriculture, 14(1): 88-97.
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| Bailey K L, Lazarovits G. 2003. Suppressing soil-borne diseaseswith residue management and organic amendments. Soiland Tillage Research, 72, 169–180.Bithell S L, Butler R C, Harrow S, McKay A, Cromey M G.2011. Susceptibility to take-all of cereal and grass species,and their effects on pathogen inoculum. Annals of AppliedBiology, 159, 252–266.Cao K Q, Wang S T. 2007. Autotoxictiy and soil sickness ofstrawberry (Fragaria ananassa). Allelopathy Journal, 20,103–114.Chen Y X, Tang W H, Zhang D H, Jian X Y. 1986. A preliminaystudy on etiology of sharp eyespot of wheat in China. ActaPhytophylacica Sinica, 13, 39–44. (in Chinese)Cheng Z H, Xu P. 2012. GC-MS identification of chemicals inlily root exudates. Journal of Northwest A&F University, 40,202–208. (in Chinese)Chou C H, Lin H J. 1976. Autointoxication mechanism of Oryzasative I.Phytotoxic effects of decomposing rice residues insoil. Journal of Chemical Ecology, 2, 353–367.Chou C H, Patrick Z A. 1976. Identification and phototoxicactivity of compounds produced during decomposition ofcorn and rye residues in soil. Journal of Chemical Ecology,2, 369–387.Cui C, Cai J, Zhang S X. 2013. Allelopathic effects of walnut(Juglans regia L.) rhizospheric soil extracts on germinationand seedling growth of turnip (Brassica rapa L.). AllelopathyJournal, 32, 37–47.Daval S, Lebreton L, Gazengel K, Boutin M, Guillerm-Erckelboudt A Y, Sarniguet A. 2011. The biocontrolbacterium Pseudomonas fluorescens Pf29Arp strain affectsthe pathogenesis-related gene expression of the take-allfungus Gaeumannomyces graminis var. tritici on wheatroots. Molecular Plant Pathology, 12, 839–854.Dong N, Liu X, Lu Y, Du L P, Xu H J, Liu H X, Xin Z Y, Zhang ZY. 2010. Overexpression of TaPIEP1, a pathogen-inducedERF gene of wheat, confers host-enhanced resistanceto fungal pathogen Bipolaris sorokiniana. Functional &Integrative Genomics, 10, 215–226.Fan K, Zhang X D, Yu X M, Yang J X, Xin L. 2013. Biologicalcharacteristics of Colletotrichum gloeosporioides andinhibitory effects of eight fungicides. Acta PhytopathologicaSinica, 43, 75–81.Gao F, Jia Z K, Lu W T, Han Q F, Yang B P, Hou X Q. 2011.Effects of different straw returning treatments on soil water,maize growth and photosynthetic characteristics in thesemi-arid area of Southern Ningxia. Acta Ecologica Sinica,31, 0777–0783.Geng G D, Zhang S Q, Cheng Z H. 2009. Allelopathy andallelochemicals of root exudates in hot pepper. ActaHorticulturae Sinica, 36, 873–878. (in Chinese)Gill J S, Sivasithamparam K, Smettem K R J. 2001. Soil moistureaffects disease severity and colonization of wheat roots byRhizoctonia solani AG-8 Soil Biology and Biochemistry,33, 1363–1370Grzegorz L, Hanna K. 2013. Effects of sharp eyespot(Rhizoctonia cerealis) on yield and grain quality of winterwheat. European Journal Plant Pathology, 135, 187–200.Hamada M S, Yin Y N, Chen H G, Ma Z H. 2011. Theescalating threat of Rhizoctonia cerealis, the causal agentof sharp eyespot in wheat. Pest Management Science, 67,1411–1419.Han X, Cheng Z H, Meng H W, Yang X L, Ahmad I. 2013.Allelopathic effect of decomposed garlic (Allium sativum L.)stalk on lettuce (L. sativa var. crispa L.). Pakistan Journalof Botany, 45, 225–233.Hao W Y, Ran W, Shen Q R, Ren L X. 2010. Effects of rootexudates from watermelon, rice plants and phenolic acidson Fusarium oxysporum f. sp. niveum. Scientia AgriculturaSinica, 43, 2443–2452. (in Chinese)Huang Y Q, Han X R, Yang J F, Liang C H, Zhan X M. 2013. Autotoxicity of peanut and identification of phytotoxicsubstances in rhizosphere soil. Allelopathy Journal, 31,297–308.Hou Y X, Zhou B L, Wu X L, Fu Y W, Wang Y Y. 2006.Allelopathy of decomposing pepper stalk on pepper growth.Chinese Journal of Applied Ecology, 17, 699–702. (inChinese)Jia T X, Wu G B, Liu C D. 1995. The present research situationand control countermeasure of root rots in wheat. ScientiaAgricultura Sinica, 28, 41–48. (in Chinese)Jiang G Y, Li Y B, Liu J G. 2013. Autotoxicity potential of cottontissues and root exudates and identification of its autotoxins.Allelopathy Journal, 32, 279–287.Kumar J, Schafer P, Hückelhoven R, Langen G, BaltruschatH, Stein E, Nagarajan S, Kogel K H. 2002. Bipolarissorokiniana, a cereal pathogen of global concern: cytologicaland molecular approaches towards better control. MolecularPlant Pathology, 3, 185–195.Li X G, Zhang T L, Wang X X, Hua K, Zhao L, Han Z M.2013. The composition of root exudates from two differentresistant peanut cultivars and their effects on the growthof soil-borne pathogen. International Journal of BiologicalScience, 9, 164–173.Li Y B, Wan C X, Zhang Q, Gong M F, Zhang L L. 2009.Effect of decomposed liquids of cotton stalk on cottonseeds germination and seedling growth. Journal of Agro-Environment Science, 28, 1258–1262.Liu H J, Huang R N, Xie F, Zhang S X, Shi J. 2012.Enantioselective phytotoxicity of metolachlor againstmaize and rice roots. Journal of Hazardous Materials, 217,330–337.Liu P, Jiang L H, Wan S P, Wei J L, Yu S F, Yang L, Wang M.2009. Studies on allelopathy of peanut root exudates onroot rot fungi and N-fixing bacteria. Journal of AgriculturalScience and Technology, 11, 107–111. (in Chinese)Liu P, Liu Z H, Wang C B, Guo F, Wang M, Zhang Y F, DongL, Wan S B. 2012. Effects of three long-chain fatty acidspresent in peanut (Arachis hypogaea L.) root exudates onits own growth and the soil enzymes activities. AllelopathyJournal, 29, 13–24.Martin V L, McCoy E L, Dick W A. 1990. Allelopathy of cropresidues influences com seed germination and early growth.Agronomy Journal, 82, 555–560.Peeyush S, Vikas A, Sharma R K. 2011. Impact of tillage andmulch management on economics, energy requirementand crop performance in maize-wheat rotation in rainfedsubhumid inceptions, India. European Journal of Agronomy,34, 46–51.Porter P M, Banwart W L, Hassett J J. 1985. HPLC isolation andGC-MS identification of genistein, daidzein and coumestrolfrom unhydrolyzed soybean root extracts. Environmentaland Experimental Botany, 25, 29–232.Rice E L. 1984. Allelopathy. 2nd ed. Academic Press, UnitedStates. pp. 1–50.Röhrig J, Kastner C, Fischer R. 2013. Light inhibits sporegermination through phytochrome in Aspergillus nidulans.Current Genetics, 59, 55–62.Sun H Y, Wang Y. 2012. Effect of root exudated potentialallelochemicals in hot pepper (Capsicum annumm L.) onantioxidative metabolism for Lettuce (Lactuca sativa L.).Plant Physiology Journal, 48, 887–894. (in Chinese)Wang M, Lü B L, Xing X P, Li H L. 2011. Composition andvirulence variation of the pathogen of wheat sharp eyespotfrom Henan Province. Acta Phytopathologica Sinica, 41,556–560.Wang J, Zhu J Y, Gao Y N, Liu B Y, Liu S P, He F, Wu Z B.2013. Toxicity of allelochemicals released by submergedmacrophytes on phytoplankton. Allelopathy Journal, 31,199–210.Williamson G B, Richardson D. 1988. Bioassays for allelopathy:Measuring treatment responses with independent controls.Journal of Chemical Ecology, 14, 181–187.Wit C, Cassman K G, Olk D C. 2000. Crop rotation and residuemanagement effects on carbon sequestration, nitrogencycling and productivity of irrigated rice systems. PlantSoil, 225, 263–278.Wu Y X, Shen X J, Fang W P. 2007. The effects of cotton rootexudates on growth and development of Verticillium dahliae.Cotton Science, 19, 286–290.Xie R Z, Li S K, Li X J, Jin Y Z, Wang K R, Chu Z D, Gao SJ. 2007. The analysis of conservation tillage in Chinaconservationtillage and crop production: Reviewing theevidence. Scientia Agricultura Sinica, 40, 1914–1924. (inChinese)Yang L H, Wang J F, Du L P, Xu H J, Wei X N, Li Z, Ma LJ, Zhang Z Y. 2013. Generation and characterization ofPgPRIP1 transgenic wheat plants with enhanced resistanceto take-all and common root rot. Acta Agronomica Sinica,39, 1576–1581. (in Chinese)Yang M M, Mavrodi D V, Mavrodi O V, Bonsall R F, ParejkoJ A, Paulitz T C, Thomashow L S, Yang H T, Weller D M,Guo J H. 2011. Biological control of take-all by fluorescentPseudomonas spp. from Chinese wheat fields. BiologicalControl, 101, 1481–1491.Ye S F, Yu J Q, Peng Y H, Zheng J H, Zou L Y. 2004. Incidenceof Fusarium wilt in Cucumis sativus L. is promoted bycinnamic acid, an autotoxin in root exudates. Plant andSoil, 263, 143–150.Ye S F, Zhou Y H, Sun Y, Zou L Y, Yu J Q. 2006. Cinnamic acidcauses oxidative stress in cucumber roots, and promotesincidence of Fusarium wilt. Environmental and ExperimentalBotany, 56, 255–262.Yu J Q, Matsui Y. 1997. Effects of root exudates of cucumber(Cucumis sativus L.) and allelochemicals on ion uptakeby cucumber seedling. Journal of Chemical Ecology, 23,817–827.Yu J Q, Matsui Y. 1999. Autointoxication of roots exudates inPisum sativus. Acta Horticulturace Sinica, 26, 175–179.(in Chinese)Zhang Q, Li Y B, Teng L P, Yue J. 2010. Allelopathy of differentdecomposed liquids of cotton stalk on Fusarium oxysporumand Verticillium dahliae. Journal of Agro-Environment Science, 31, 1696–1701. (in Chinese)Zhang X S, Cao Y S, Cao K Q. 2005. Management of wheatsoil-borne diseases under the conservative farming system.Journal of Northwest Sci-Tech University of Agriculture andForestry, 33, 47–48. (in Chinese)Zhang Y M, Ma Y Q. 1994. Alleopathic effect of wheat strawmulching on seedling growth and development of summercorn. Chinese Journal of Ecology, 13, 70–72. (in Chinese)Zhao X S, Zhen W C, Qi Y Z, Liu X J, Yin B Z. 2009. Coordinatedeffects of root autotoxic substances and Fusariumoxysporum Schl. f. sp. fragariae on the growth and replantdisease of strawberry. Frontiers of Agriculture in China,3, 34–39.Zhou B L, Chen Z X, Du L, Xie Y H, Zhang Q, Ye X L. 2011.Allelopathy of root exudates from different resistanteggplants to Verticillium dahliae and the identification ofallelochemicals. African Journal of Biotechnology, 10,8284–8290.Zhou B L, Yin Y L, Li Y P, Xu Y, Chen S L, Ye X L. 2010.Relationship between root exudates of grafted eggplantsand Verticillium wilt resistance and their componentsidentification. Acta Ecologica Sinica, 30, 3073–3079. (inChinese) |
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