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| Molecular mapping of a stripe rust resistance gene in Chinese wheat cultivar Mianmai 41 |
| REN Yong, LI Sheng-rong, WEI Yu-ming, ZHOU Qiang, DU Xiao-ying, HE Yuan-jiang, ZHENG You-liang |
1、Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2、Mianyang Institute of Agricultural Science/Mianyang Wheat Experimental Station, China Agriculture Research System (CARS),
Mianyang 621023, P.R.China |
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摘要 Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2:3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as YrMY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 cM, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and YrCH42 on chromosome 1B. Allelic tests indicated that YrMY41 is likely a new allele at Yr26 locus.
Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2:3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as YrMY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 cM, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and YrCH42 on chromosome 1B. Allelic tests indicated that YrMY41 is likely a new allele at Yr26 locus.
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Received: 10 February 2014
Accepted:
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| Fund: This work was supported by the China Agriculture Research System (CARS- 03) and the National Basic Research Program of China (2011CB100100). |
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Corresponding Authors:
ZHENG You-liang, Tel: +86-28-86290909,Fax: +86-28-82650350, E-mail: ylzheng@sicau.edu.cn
E-mail: ylzheng@sicau.edu.cn
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| About author: REN Yong, Mobile: 13698123162, E-mail: rywheat@126.com; |
Cite this article:
REN Yong, LI Sheng-rong, WEI Yu-ming, ZHOU Qiang, DU Xiao-ying, HE Yuan-jiang, ZHENG You-liang.
2015.
Molecular mapping of a stripe rust resistance gene in Chinese wheat cultivar Mianmai 41. Journal of Integrative Agriculture, 14(2): 295-304.
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