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Journal of Integrative Agriculture  2017, Vol. 16 Issue (10): 2145-2155    DOI: 10.1016/S2095-3119(16)61564-2
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Conditional and unconditional QTLs mapping of gluten strength in common wheat (Triticum aestivum L.)
LIU Tong-tong, LIU Kai, WANG Fang-fang, ZHANG Ying, LI Qing-fang, ZHANG Kai-ran, XIE Chu-peng, TIAN Ji-chun, CHEN Jian-sheng
State Key Laboratory of Crop Biology/Group of Wheat Quality Breeding, Key Laboratory of Crop Water Physiology and Drought-tolerance Germplasm Improvement, Ministry of Agriculture/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
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Abstract      Dissecting the genetic relationships among gluten-related traits is important for high quality wheat breeding. Quantitative trait loci (QTLs) analysis for gluten strength, as measured by sedimentation volume (SV) and gluten index (GI), was performed using the QTLNetwork 2.0 software. Recombinant inbred lines (RILs) derived from the winter wheat varieties Shannong 01-35×Gaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identified using an unconditional analysis. QGi1D-13 and QSv1D-14 were detected through unconditional and conditional QTLs mapping, which explained 9.15–45.08% of the phenotypic variation. QTLs only identified under conditional QTL mapping were located in three marker intervals: WPT-3743–GLU-D1 (1D), WPT-7001–WMC258 (1B), and WPT-8682–WPT-5562 (1B). Six pairs of epistatic QTLs distributed nine chromosomes were identified. Of these, two main effect QTLs (QGi1D-13 and QSv1D-14) and 12 pairs of epistatic QTLs were involved in interactions with the environment. The results indicated that chromosomes 1B and 1D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level.
Keywords:  wheat (Triticum aestivum L.)        gluten strength        gluten index        sedimentation volume        unconditional QTL mapping        conditional QTL mapping  
Received: 02 November 2016   Accepted:

This work received support from the Natural Science Foundation of Shandong Province, China (ZR2015CM036), the Molecular Foundation of Main Crop Quality, the Ministry of Science and Technology of China (2016YFD0100500), the Project of Science and Technology of Shandong “Wheat Breeding by Molecular Design”, China (2016LZGC023), and the Research Fund for Agricultural Big Data Project, China.

Corresponding Authors:  Correspondence CHEN Jian-sheng, Tel: +86-538-8249236, E-mail:; TIAN Ji-chun, Tel/Fax: +86-538-8242040, E-mail:    
About author:  LIU Tong-tong, E-mail:

Cite this article: 

LIU Tong-tong, LIU Kai, WANG Fang-fang, ZHANG Ying, LI Qing-fang, ZHANG Kai-ran, XIE Chu-peng, TIAN Ji-chun, CHEN Jian-sheng. 2017. Conditional and unconditional QTLs mapping of gluten strength in common wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 16(10): 2145-2155.

AACC International. 2000. Approved methods of the American Association of Cereal Chemists. 10th ed., Methods  26-21A and 56-61A. The American Association of Cereal Chemists, St. Paul.

Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S Y, Szynski G U, Mohler V, Lehmensiek A, Kuchel H, Hayden M J, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A. 2006. Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theoretical and Applied Genetics, 113, 1409–1420.

Clarke F R, Clarke J M, Ames N A, Knox R E, Ross J R. 2010. Gluten index compared with SDS-sedimentation volume for early generation selection for gluten strength in durum wheat. Canadian Journal of Plant Pathology, 90, 1–11.

Clarke J M, Clarke F R, McCaig T N, Knox R E, Ames N P. 2000. Evaluation of predictors of quality for use in early generation selection. In: Royo C, Nachit M M, Fonzo N, Araus J L, eds., Durum Wheat Improvement in the Mediterranean Region: New Challenges. Zaragoza, Spain. pp. 439–446.

Conti V, Roncallo P F, Beaufort V, Cervigni G L, Miranda R, Jensen C A, Echenique V C. 2011. Mapping of main and epistatic effect QTLs associated to grain protein and gluten strength using a RIL population of durum wheat. Journal of Applied Genetics, 52, 287–298.

Cubbada R, Carcea M, Pasqui L A. 1992. Suitability of the gluten index method for assessing gluten strength in durum wheat and semolina. Cereal Food World, 37, 866–869.

Cui F, Li J, Ding A M, Zhao C H, Wang L, Wang X Q, Li S S, Bao Y G, Li X F, Feng D S, Kong L R, Wang H G. 2011. Conditional QTL mapping for plant height with respect to the length of the spike and internode in two mapping populations of wheat. Theoretical and Applied Genetics, 122, 1517–1536.

Deng Z Y, Tian J C, Chen F, Li W J, Zheng F F, Chen J S, Shi C L, Sun C L, Wang S Y, Zhang Y X. 2014. Genetic dissection on wheat flour quality traits in two related populations. Euphytica, 203, 221–235.

Deng Z Y, Zhao L, Liu B, Zhang K P, Chen J S, Qu H L, Sun C L, Zhang Y X, Tian J C. 2013. Conditional QTL mapping of sedimentation volume on seven quality traits in common wheat. Journal of Integrative Agriculture, 12, 2125–2133.

Doerge R W. 2002. Mapping and analysis of quantitative trait loci in experimental populations. Nature Reviews Genetics, 3, 43–52.

Edwards N M, Gianibelli M C, McCaig T N, Clarke J M, Ames N P, Larroque O R, Dexter J E. 2007. Relationships between dough strength, polymeric protein quantity and composition for diverse durum wheat genotypes. Journal of Cereal Science, 45, 140–149.

Elangovan M, Rai R, Dholakia B B, Lagu M D, Tiwari R, Gupta R K, Rao V S, Röder M S, Gupta V S. 2008. Molecular genetic mapping of quantitative trait loci associated with loaf volume in hexaploid wheat (Triticum aestivum). Journal of Cereal Science, 47, 587–598.

He Z H, Yang J, Zhang Y, Quail K J, Peña R J. 2004. Pan bread and dry white Chinese noodle quality in Chinese winter wheat. Euphytica, 139, 257–267.

Huang X Q, Cloutier S, Lycar L, Radovanovic N, Humphreys D G, Noll J S, Somers D J, Brown P D. 2006. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theoretical and Applied Genetics, 113, 753–766.

Kerfal S, Giraldo P, Rodríguez-Quijano M, Vázquez J F, Adams K, Lukow O M, Röder M S, Somers D J, Carrillo J M. 2010. Mapping quantitative trait loci (QTLs) associated with dough quality in a soft×hard bread wheat progeny. Journal of Cereal Science, 52, 46–52.

Kosambi D D. 1943. The estimation of map distances from recombination values. Annals of Human Genetics, 12, 172–175.

Kumar A, Elias E M, Ghavami F, Xu X, Jain S, Manthey F A, Mohammed S A, Penny M A, Kianian S F. 2013. A major QTL for gluten strength in durum wheat (Triticum turgidum L. var. durum). Journal of Cereal Science, 57, 21–29.

Kunert A, Naz A A, Oliver D, Pillen K, Léon J. 2007. AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides×T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theoretical and Applied Genetics, 115, 683–695.

Li Y, Song Y, Zhou R, Branlard G, Jia J. 2009. Detection of QTLs for bread-making quality in wheat using a recombinant inbred line population. Plant Breeding, 128, 235–243.

Li Y, Zhou R, Wang J, Liao X, Branlard G, Jia J. 2012. Novel and favorable QTL allele clusters for end-use quality revealed by introgression lines derived from synthetic wheat. Molecular Breeding, 29, 627–643.

Lincoln S, Daly M, Lander E. 1992. Constructing genetics maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report. Whitehead Institute, Cambridge, Massachusetts, USA.

Liu L, He Z H, Yan J, Zhang Y, Xia X C, Peña R J. 2005. Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica, 142, 197–204.

Ozturk S, Kahraman K, Tiftik B, Koksel H. 2008. Predicting the cookie quality of flours by using Mixolab. European Food Research and Technology, 227, 1549–1554.

Patil R M, Oak M D, Tamhankar S A, Rao V S. 2009. Molecular mapping of QTLs for gluten strength as measured by sedimentation volume and mixograph in durum wheat (Triticum turgidum L. ssp. durum). Journal of Cereal Science, 49, 378–386.

Pestsova E, Ganal M W, Röder M S. 2000. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 43, 689–697.

Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal W M. 1998. A microsatellite map of wheat. Genetics, 149, 2007–2023.

Sun H Y, Lü J H, Fan Y D, Zhao Y, Kong F M, Li R J, Wang H G, Li S S. 2008. Quantitative trait loci (QTLs) for quality traits related to protein and starch in wheat. Progress in Natural Science, 18, 825–831.

Tsilo T J, Simsek S, Ohm J B, Hareland G A, Chao S, Anderson J A. 2011. Quantitative trait loci influencing endosperm texture, dough-mixing strength, and bread-making properties of the hard red spring wheat breeding lines. Genome, 54, 460–470.

Voorrips R E. 2002. MapChart, software for the graphical presentation of linkage maps and QTLs. Journal of Heredity, 93, 77–78.

Wang D L, Zhu J, Li Z K, Paterson A H. 1999. Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. Theoretical and Applied Genetics, 99, 1255–1264.

Wang L H, Li G Y, Peña R J, Xia X C, He Z H. 2010. Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). Journal of Cereal Science, 51, 305–312.

Wang Z, Wu X, Ren Q, Chang X, Li R, Jiang R. 2010. QTL mapping for developmental behavior of plant height in wheat (Triticum aestivum L.). Euphytica, 174, 447–458.

Wen Y X, Zhu J. 2005. Multivariable conditional analysis for complex trait and its components. Journal of Genetics and Genomics, 32, 289–296.

Wyman E, Nyquist R J, Baker. 1991. Estimation of heritability and prediction of selection response in plant populations. Critical Reviews in Plant Sciences, 10, 235–322.

Yang J, Zhu J. 2005. Methods for predicting superior genotypes under multiple environments based on QTL effects. Theoretical and Applied Genetics, 110, 1268–1274.

Yang J, Zhu J, Williams R W. 2007. Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics, 23, 1527–1536.

Zhu J. 1995. Analysis of conditional genetic effects and variance components in developmental genetics. Genetics, 141, 1633–1639.
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