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Journal of Integrative Agriculture  2017, Vol. 16 Issue (10): 2145-2155    DOI: 10.1016/S2095-3119(16)61564-2
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Conditional and unconditional QTLs mapping of gluten strength in common wheat (Triticum aestivum L.)
LIU Tong-tong, LIU Kai, WANG Fang-fang, ZHANG Ying, LI Qing-fang, ZHANG Kai-ran, XIE Chu-peng, TIAN Ji-chun, CHEN Jian-sheng
State Key Laboratory of Crop Biology/Group of Wheat Quality Breeding, Key Laboratory of Crop Water Physiology and Drought-tolerance Germplasm Improvement, Ministry of Agriculture/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
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Abstract      Dissecting the genetic relationships among gluten-related traits is important for high quality wheat breeding. Quantitative trait loci (QTLs) analysis for gluten strength, as measured by sedimentation volume (SV) and gluten index (GI), was performed using the QTLNetwork 2.0 software. Recombinant inbred lines (RILs) derived from the winter wheat varieties Shannong 01-35×Gaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identified using an unconditional analysis. QGi1D-13 and QSv1D-14 were detected through unconditional and conditional QTLs mapping, which explained 9.15–45.08% of the phenotypic variation. QTLs only identified under conditional QTL mapping were located in three marker intervals: WPT-3743–GLU-D1 (1D), WPT-7001–WMC258 (1B), and WPT-8682–WPT-5562 (1B). Six pairs of epistatic QTLs distributed nine chromosomes were identified. Of these, two main effect QTLs (QGi1D-13 and QSv1D-14) and 12 pairs of epistatic QTLs were involved in interactions with the environment. The results indicated that chromosomes 1B and 1D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level.
Keywords:  wheat (Triticum aestivum L.)        gluten strength        gluten index        sedimentation volume        unconditional QTL mapping        conditional QTL mapping  
Received: 02 November 2016   Accepted:
Fund: 

This work received support from the Natural Science Foundation of Shandong Province, China (ZR2015CM036), the Molecular Foundation of Main Crop Quality, the Ministry of Science and Technology of China (2016YFD0100500), the Project of Science and Technology of Shandong “Wheat Breeding by Molecular Design”, China (2016LZGC023), and the Research Fund for Agricultural Big Data Project, China.

Corresponding Authors:  Correspondence CHEN Jian-sheng, Tel: +86-538-8249236, E-mail: cjs777777@126.com; TIAN Ji-chun, Tel/Fax: +86-538-8242040, E-mail: jctian9666@163.com    
About author:  LIU Tong-tong, E-mail: 13617314043@163.com

Cite this article: 

LIU Tong-tong, LIU Kai, WANG Fang-fang, ZHANG Ying, LI Qing-fang, ZHANG Kai-ran, XIE Chu-peng, TIAN Ji-chun, CHEN Jian-sheng. 2017. Conditional and unconditional QTLs mapping of gluten strength in common wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 16(10): 2145-2155.

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