Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents
TAN Xiao-li, ZHENG Xiang-feng, ZHANG Zhi-yan, WANG Zheng, XIA Heng-chuan, LU Changming, GU Shou-lai
1.Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R.China
2.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R.China
摘要 Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol (TAG) biosynthesis and fatty acid β-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassica napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum (ER), the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.
Abstract Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol (TAG) biosynthesis and fatty acid β-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassica napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum (ER), the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.
This work was supported by the Natural Science Foundation of China (31271760) and the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops, Ministry of Agriculture, China.
TAN Xiao-li, ZHENG Xiang-feng, ZHANG Zhi-yan, WANG Zheng, XIA Heng-chuan, LU Changming, GU Shou-lai.
2014.
Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents. Journal of Integrative Agriculture, 13(1): 54-62.
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