|
|
|
Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.) |
CHAILu1 , ZHANGJian1 , PANXiao-biao2 , ZHANGFan1 , ZHENGTian-qing1 , ZHAOXiu-qing1 , WANGWen-sheng1 , AliJauhar3 , XUJian-long1 , LIZhi-kang1 |
1、National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Crop Research Institute, Taizhou Academy of Agricultural Sciences of Zhejiang Province, Linhai 317000, P.R.China
3、International Rice Research Institute, DAPO 777, Metro Malina, The Philippines |
|
|
摘要 Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the whole- plant growth duration salt tolerance (ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines (ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL (AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and non- stress conditions were identified, most (>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan (HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection (MAS) or genome selection.
Abstract Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the whole- plant growth duration salt tolerance (ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines (ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL (AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and non- stress conditions were identified, most (>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan (HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection (MAS) or genome selection.
|
Received: 17 May 2013
Accepted:
|
Fund: Acknowledgements This research study was funded by the National High-Tech R&D Program of China (2012AA101101), the 948 Project from the Ministry of Agriculture, China (2010-G2B), the International Cooperative Project from the Ministry of Science and Technology, China (S2012ZR0160), and the Bill & Melinda Gates Foundation Project (OPP51587). |
Corresponding Authors:
LI Zhi-kang, Tel: +86-10-82105857, E-mail: zhkli1953@126.com; XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@126.com
E-mail: zhkli1953@126.com;xujlcaas@126.com
|
Cite this article:
CHAILu1 , ZHANGJian1 , PANXiao-biao2 , ZHANGFan1 , ZHENGTian-qing1 , ZHAOXiu-qing1 , WANGWen-sheng1 , AliJauhar3 , XUJian-long1 , LIZhi-kang1 .
2014.
Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.). Journal of Integrative Agriculture, 13(8): 1609-1620.
|
Akbar M, Yabuno T. 1974. Breeding for saline-resistant varieties of rice: II. Comparative performances of some rice varieties to salinity during early development stage. Japanese Journal Breeding, 25, 176-181 Ali A J, Xu J L, Ismail A M, Fu B Y, Vijayakumar C H M, Gao Y M, Domingo J, Maghirang R, Yu S B, Gregorio G, Yanaghihara S, Cohen M, Carmen B, Mackill D, Li Z K. 2006. Hidden diversity for abiotic and biotic stress tolerances in the primary gene pool of rice revealed by a large backcross breeding program. Field Crops Research, 97, 66-76 Andaya V C, Tai T H. 2006. Fine mapping of the qCTS12 locus, a major QTL for seedling cold tolerance in rice. Theoretical and Applied Genetics, 113, 467-475 Churchill G A, Doerge R W. 1994. Empirical threshold values for quantitative trait mapping. Genetics, 138, 963-971 Foolad M R, Lin G Y. 1997. Absence of a relationship between salt tolerance during germination and vegetative growth in tomato. Plant Breeding, 116, 363-367 Greenway H, Munns R. 1980. Mechanism of salt tolerance in non-halophytes. Annual Review of Plant Physiology, 31, 149-190 He Y X, Zheng T Q, Hao X B, Wang L F, Gao Y M, Hua T Z, Zhai H Q, Xu J L, Xu J Z, Zhu L H, Li Z K. 2010. Yield performances of japonica introgression lines selected for drought tolerance in a BC breeding programme. Plant Breeding, 129, 167-175 Hou X, Xie K B, Yao J L, Qi Z Y, Xiong L Z. 2009. A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance. Proceedings of the National Academy of Sciences of the United States of America, 106, 6410-6415 Jiang L, Xun M M, Wang J K, Wan J M. 2008. QTL analysis of cold tolerance at seedling stage in rice (Oryza sativa L.) using recombination inbred lines. Journal of Cereal Science, 48, 173-179 Johnson D W, Smith S E, Dobrenz A K. 1992. Genetic and phenotypic relationships in response to NaCl at different developmental stages in alfalfa. Theoretical and Applied Genetics, 83, 833-838 Kanneganti V, Gupta A K. 2008. Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice. Plant Molecular Biology, 66, 445-462 Koyama M L, Levesley A, Koebner R M, Flowers T J, Yeo A R. 2001. Quantitative trait loci for component physiological traits determining salt tolerance in rice. Plant Physiology, 125, 406-422 Lafitte H R, Li Z K, Vijayakumar CH M, Gao Y M, Shi Y, Xu J L, Fu B Y. 2006. Improvement of rice drought tolerance through backcross breeding: evaluation of donors and selection in drought nurseries. Field Crops Research, 97, 77-86 Lauchli A, Epstein E. 1990. Plant responses to saline and sodic conditions. In: Tanji K K, ed., Agricultural Salinity Assessment and Management. American Society of Civil Engineers, New York. pp. 113-137 Lee S Y, Ahn J H, Cha Y S, Yun D W, Lee M C, Ko J C, Lee K S, Eun M Y. 2006. Mapping of quantitative trait loci for salt tolerance at the seedling stage in rice. Molecular Cells, 21, 192-196 Li H H, Ye G Y, Wang J K. 2007. A modified algorithm for the improvement of composite interval mapping. Genetics, 175, 361-374 Li Z K. 2001. QTL mapping in rice: A few critical considerations. In: Khush G S, Brar D S, Hardy B, eds., Rice Genetics IV. Science Publishers, and International Rice Research Institute, New Delhi, India, and Los Banos, Philippines. pp. 153-172 Li Z K, Fu B Y, Gao Y M, Xu J L, Ali J, Lafitte H R, Jiang Y Z, Domingo R J, Vijayakumar CH M, Maghirang R, Zheng T Q, Zhu L H. 2005. Gonome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.). Plant Molecular Biology, 59, 33-52 Li Z K, Zhang F. 2013. Rice breeding in the post-genomics era: From concept to practice. Current Opinion in Plant Biology, doi: org/10.1016/j.pbi.2013.03.008 Lin H X, Zhu M Z, Yano M, Gao J P, Liang Z W, Su W A, Hu X H, Ren Z H, Chao D Y. 2004. QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance. Theoretical and Applied Genetics, 108, 253-260 Ma B J, Gu Z M, Tang H J, Chen X F, Liu F, Zhang H S. 2009. Preliminary study on the function of calcineurin B-like protein gene OsCBL8 in rice. [2013-02-13] Sciencepaper online of China. http://www.paper.edu.cn/releasepaper/ content/200901-151 Mather K, Jinks J L 1982. Biometrical Genetics. 3rd ed. Chapman and Hall, London. Meng L J, Lin, X Y, Wang J M, Chen K, Cui Y R, Xu J L, Li Z K. 2013. Simultaneous improvement of cold tolerance and yield of temperate japonica rice (Oryza sativa L.) by introgression breeding. Plant Breeding, 132, 604-612 Prasad S R, Bagali P G, Hittalmani S, Shashidhar H E. 2000. Molecular mapping of quantitative trait loci associated with seedling tolerance to salt stress in rice (Oryza sativa L.). Current Science, 78, 162-164 Rajanaidu N, Zakri A H. 1988. Breeding for morphophysiological traits in crop plants. In: Zakri A H, ed., Plant Breeding and Genetic Engineering. SABRAO Publishers. pp. 116-139 SAS Institute. 1996. SAS/STAT User’s Guide. SAS Institute, Cary NC, USA. pp. 25-36 Senadhira D, Zapata-Arias F J, Gregorio G B, Alejar M S, de la Cruz H C, Padolina T F, Galvez A M. 2002. Development of the first saly-tolerant rice cultivar through indica/indica anther culture. Field Crops Research, 76, 103-110 Shannon M C. 1985. Principles and strategies in breeding for higher salt tolerance. Plant and Soil, 89, 227-241 Sun Y, Zang J P, Wang Y, Zhu L H, Fotokian M, Xu J L, Li Z K. 2007. Mining favorable salt-tolerant QTL from rice germplasm using a backcrossing introgression line population. Acta Agronomica Sinica, 33, 1611-1617 (in Chinese) Takehisa H, Shimodate T, Fukuta Y, Ueda T, YanoM, Yamaya T, Kameya T, Sato T. 2004. Identification of quantita-tive trait loci for plant growth of rice in paddy field flooded with salt water. Field Crops Research, 89, 85-95 Tal M. 1985. Genetics of salt tolerance in higher plants: Theoretical and applied considerations. Plant Soils, 89, 199-226 Tanksley S D, Nelson J C. 1996. Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theoretical and Applied Genetics, 92, 191-203 Temnykh S, DeCklerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S. 2001. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential. Genome Research, 11, 1441-1452 Tian L, Tan L B, Liu F X, Cai H W, Sun C Q. 2011. Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon. Journal of Genetics and Genomics, 38, 593-601 Walia H, Wilson C, Condamine P, Liu X, Ismail A M, Zeng L, Wanamaker S I, Mandal J, Xu J, Cui X, Close T J. 2005. Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiology, 139, 822-835 Walia H, Wilson C, Zeng L, Ismail A M, Close P C, Close T J. 2007. Genome-wide transcriptional analysis of salinity stressed japonica and indica rice genotypes during panicle initiation stage. Plant Molecular Biology, 63, 609-623 Weir B S. 1996. Genetic Data Analysis II: Methods for Discrete Population Genetic Data. 2nd ed. Sinauer Associates, Sunderland, MA. Xu J L, Lafitte H R, Gao Y M, Fu B Y, Torres R, Li Z K. 2005. QTLs for drought escape and tolerance identified in a set of random introgression lines of rice. Theoretical and Applied Genetics, 111, 1642-1650Xu J L, Wang J M, Sun Y Q, Wei L J, Luo R T, Zhang MX, Li Z K. 2006. Heavy genetic load associated with thesubspecific differentiation of Japonica Rice (Oryza sativassp. Japonica L.). Journal of Experimental Botany, 57,2815-2824Yeo A R, Yeo M E, Flower S A, Flowers T J. 1990. Screeningof rice (Oryza sativa L.) genotypes for physiologicalcharacters contributing to salinity resistance, and theirrelationship to overall performance. Theoretical andApplied Genetics, 79, 377-384Yeo A R. 1994. Physiological criteria in screening andbreeding. In: Yeo A R, Flowers T J, eds., Soil MineralStresses: Approaches to Crop Improvement. Springer Verlag, Berlin. pp. 37-57Zaidem M L, Mendoza R D, Tumimbang E B. 2004. Geneticvariability of salinity tolerance at different growth stagesof rice. In: PBGB 2003 Annual Report. Las Banos, IRRI,Philippines. pp. 19-20Zang J P, Sun Y, Wang Y, Yang J, Li F, Zhou Y L, Zhu LH, Reys J, Mohammadhosein F, Xu J L, Li Z K. 2008.Dissection of genetic overlap of salt tolerance QTLs at theseedling and tillering stage using backcross introgressionlines in rice. Science in China (Series C: Life Sciences),51, 583-591Zhang F, Jiang Y Z, Yu S B, Ali J, Paterson A H, Khush G S,Xu J L, Gao Y M, Fu B Y, Lafitte R, Li Z K. 2013. Threegenetic systems controlling growth, development andproductivity of rice (Oryza sativa L.): A reevaluation ofthe Green Revolution. Theoretical and Applied Genetics,126, 1011-1024Zhang F, Zhai H Q, Paterson A H, Xu J L, Gao Y M, Zheng TQ, Wu R L, Fu B Y, Ali J, Li Z K. 2011. Dissecting genetic networks underlying complex phenotypes: The theoretical framework. PLoS ONE, 6, e14541.Zhang G, Yan G, Chen S L, Chen S Y. 1995. RFLP tagging ofa salt tolerance gene. Plant Science, 110, 227-234 |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|