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| Barley and Wheat Share the Same Gene Controlling the Short Basic Vegetative Period |
| LÜ Rui-hua, XU Yan-hao, Rodger Boyd, ZHANG Xiao-qi, Sue Broughton, Michael Jones, LI Cheng-dao,CHEN Yao-feng |
1 College of Agronomy, Northwest A&F University, Shaanxi 712100, P.R.China
2 College of Agriculture, Yangtze University, Jingzhou 434000, P.R.China
3 Department of Agriculture and Food, Government of Western Australia, South Perth WA 6151, Australia
4 State Agricultural Biotechnology Centre, Murdoch University, Murdoch WA 6132, Australia
5 Natural and Agricultural Sciences, The University of Western Australia, Crawley WA 6009, Australia |
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摘要 Basic vegetative period (BVP) is an important trait for determining flowering time and adaptation to variable environments. A short BVP barley mutant is about 30 d shorter than its wild type. Genetic analysis using 557 F2 individuals revealed that the short BVP is governed by a single recessive gene (BVP-1) and was further validated in 2 090 F3 individuals. The BVP-1 gene was first mapped to barley chromosome 1H using SSR markers. Comparative genomic analysis demonstrated that the chromosome region of BVP-1 is syntenic to rice chromosome 5 and Brachypodium chromosome 2. Barley ESTs/genes were identified after comparison with candidate genes in rice and Brachypodium; seven new gene-specific markers were developed and mapped in the mapping populations. The BVP-1 gene co-segregated with the Mot1 and Ftsh4 genes and was flanked by the gene-specific markers AK252360 (0.2 cM) and CA608558 (0.5 cM). Further analysis demonstrated that barley and wheat share the same short BVP gene controlling early flowering.
Abstract Basic vegetative period (BVP) is an important trait for determining flowering time and adaptation to variable environments. A short BVP barley mutant is about 30 d shorter than its wild type. Genetic analysis using 557 F2 individuals revealed that the short BVP is governed by a single recessive gene (BVP-1) and was further validated in 2 090 F3 individuals. The BVP-1 gene was first mapped to barley chromosome 1H using SSR markers. Comparative genomic analysis demonstrated that the chromosome region of BVP-1 is syntenic to rice chromosome 5 and Brachypodium chromosome 2. Barley ESTs/genes were identified after comparison with candidate genes in rice and Brachypodium; seven new gene-specific markers were developed and mapped in the mapping populations. The BVP-1 gene co-segregated with the Mot1 and Ftsh4 genes and was flanked by the gene-specific markers AK252360 (0.2 cM) and CA608558 (0.5 cM). Further analysis demonstrated that barley and wheat share the same short BVP gene controlling early flowering.
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Received: 19 November 2012
Accepted: 01 October 2013
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| Fund: This project was supported by the Grain Research and Development Cooperation, Australia and the Chinese Scholarship Council. |
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Corresponding Authors:
Correspondence LI Cheng-dao, Tel: +61-8-93683843, E-mail: chengdao.li@
agric.wa.gov.au; CHEN Yao-feng, Mobile: 13060391299, E-mail: chenyf3828@126.com
E-mail: chengdao.li@agric.wa.gov.au
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| About author: Lü Rui-hua, E-mail: vvlv07@gmail.com; XU Yan-hao, E-mail: xyh09@yangtzeu.edu.cn |
Cite this article:
Lü Rui-hua, XU Yan-hao, Rodger Boyd, ZHANG Xiao-qi, Sue Broughton, Michael Jones, LI Cheng-dao, CHEN Yao-feng.
2013.
Barley and Wheat Share the Same Gene Controlling the Short Basic Vegetative Period. Journal of Integrative Agriculture, 12(10): 1703-1711.
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| [1]Aghnoum R, Marcel T C, Johrde A, Pecchioni N, Schweizer P, Niks R E. 2010. Basal host resistance of barley to powdery mildew: connecting quantitative trait loci and candidate genes. Molecular Plant-Microbe Interactions, 23, 91-102[2]Appels R, Francki M, Chibbar R 2003. Advances in cereal functional genomics. Functional and Integrative Genomics, 3, 1-24[3]Bäurle I, Dean C 2006. The timing of developmental transitions in plants. Cell, 125, 655-664[4]Beales J, Turner A, Griffiths S, Snape J W, Laurie D A 2007. A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 115, 721-733[5]Boyd W J R, Li C D, Grime C R, Cakir M, Potipibool S, Kaveeta L, Men S, Kamali M R J, Barr A R, Moody D B. 2003. Conventional and molecular genetic analysis of factors contributing to variation in the timing of heading among spring barley (Hordeum vulgare L.) genotypes grown over a mild winter growing season. Australian Journal of Agricultural Research, 54, 1277-1302[6]Brkljacic J, Grotewold E, Scholl R, Mockler T, Garvin D F, Vain P, Brutnell T, Sibout R, Bevan M, Budak H. 2011. Brachypodium as a model for the grasses: today and the future. Plant Physiology, 157, 3-13[7]Bullrich L, Appendino M, Tranquilli G, Lewis S, Dubcovsky J. 2002. Mapping of a thermo-sensitive earliness per se gene on Triticum monococcum chromosome 1A m. Theoretical and Applied Genetics, 105, 585-593[8]Colasanti J, Coneva V. 2009. Mechanisms of floral induction in grasses: something borrowed, something new. Plant Physiology, 149, 56-62[9]Devos K M. 2010. Grass genome organization and evolution. Current Opinion in Plant Biology, 13, 139- 145. Distelfeld A, Li C, Dubcovsky J. 2009. Regulation of flowering in temperate cereals. Current Opinion in Plant Biology, 12, 178-184[10]Faricelli M E, Valárik M, Dubcovsky J. 2010. Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium Functional and Integrative Genomics, 10, 293-306[11]Franckowiak J D, Konishi T. 2002. Early maturity 6, Eam6. Barley Genetics Newsletter, 32, 88-89[12]Fu D, Szucs P, Yan L, Helguera M, Skinner J S, Von Zitzewitz J, Hayes P M, Dubcovsky J. 2005. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat Molecular Genetics and Genomics, 273, 54-65[13]Gallagher L W, Soliman K M, Vivar H. 1991. Interactions among loci conferring photoperiod insensitivity for heading time in spring barley. Crop Science, 31, 256- 261.[14]Greenup A, Peacock W J, Dennis E S, Trevaskis B. 2009. The molecular biology of seasonal flowering-responses in Arabidopsis and the cereals. Annals of Botany, 103, 1165-1172[15]Hay R K M, Ellis R P. 1998. The control of flowering in wheat and barley: what recent advances in molecular genetics can reveal. Annals of Botany, 82, 541-554[16]Jia Q, Zhang J, Westcott S, Zhang X Q, Bellgard M, Lance R, Li C D. 2009. GA-20 oxidase as a candidate for the semidwarf gene sdw1/denso in barley Functional and Integrative Genomics, 9, 255-262[17]Jordan T, Seeholzer S, Schwizer S, Keller B. 2010. The wheat Mla homologue TmMla1 exhibits an volutionarily conserved function against powdery mildew in both wheat and barley. The Plant Journal, 65, 610-621[18]Jung C, Müller A E. 2009. Flowering time control and applications in plant breeding. Trends in Plant Science, 14, 563-573[19]Kato K, Miura H, Sawada S. 1999. Detection of an earliness per se quantitative trait locus in the proximal region of wheat chromosome 5AL. Plant Breeding, 118, 391-394[20]Kato K, Miura H, Sawada S, McIntosh R A. 2002. Characterization of QEet. ocs 5A. 1, a quantitative trait locus for ear emergence time on wheat chromosome 5AL.Plant Breeding, 121, 389-393[21]Kuraparthy V, Sood S, Gill B S. 2008. Genomic targeting and mapping of tiller inhibition gene (tin3) of wheat using ESTs and synteny with rice. Functional and Integrative Genomics, 8, 33-42[22]Laurie D A, Pratchett N, Bezant J H, Snape J W. 1995. RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome, 38, 575- 585.[23]Li C D, Ni P, Francki M, Hunter A, Zhang Y, Schibeci D, Li H, Tarr A, Wang J, Cakir M. 2004. Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Functional and Integrative Genomics, 4, 84-93[24]Liu F L, Gupta S, Zhang X Q, Jones M, Loughman R, Lance R, Li C D. 2011. PCR markers for selection of adult plant leaf rust resistance in barley (Hordeum vulgare L.). Molecular Breeding, 28, 657-666[25]Lundqvist U, Franckowiak J D, Konishi T. 1997. New and revised descriptions of barley genes. Barley Genetics Newsletter, 26, 22. [26]Mayer K F X, Martis M, Hedley P E, Šimková H, Liu H, Morris J A, Steuernagel B, Taudien S, Roessner S, Gundlach H. 2011. Unlocking the barley genome by chromosomal and comparative genomics. The Plant Cell, 23, 1249-1263[27]Sameri M, Komatsuda T. 2004. Identification of quantitative trait loci (QTLs) controlling heading time in the population generated from a cross between oriental and occidental barley cultivars (Hordeum vulgare L.). Breeding Science, 54, 327-332[28]Sameri M, Takeda K, Komatsuda T. 2006. Quantitative trait loci controlling agronomic traits in recombinant inbred lines from a cross of oriental- and occidental-type barley cultivars. Breeding Science, 56, 243-252[29]Silvar C, Perovic D, Scholz U, Casas A M, Igartua E, Ordon F. 2012. Fine mapping and comparative genomics integration of two quantitative trait loci controlling resistance to powdery mildew in a Spanish barley landrace. Theoretical and Applied Genetics, 124, 49-62[30]Snape J W, Quarrie S A, Laurie D A. 1996. Comparative mapping and its use for the genetic analysis of agronomic characters in wheat. Euphytica, 89, 27-31[31]Tsuji H, Taoka K, Shimamoto K. 2011. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation. Current Opinion in Plant Biology, 14, 45-52[32]Turner A, Beales J, Faure S, Dunford R P, Laurie D A. 2005. The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science, 310, 1031- 1034. Valárik M, Linkiewicz A M, Dubcovsky J. 2006. A microcolinearity study at the earliness per se gene Eps-A m 1 region reveals an ancient duplication that preceded the wheat-rice divergence. Theoretical and Applied Genetics, 112, 945-957[33]Vogel J P, Garvin D F, Mockler T C, Schmutz J, Rokhsar D, Bevan M W, Barry K, Lucas S, Harmon-Smith M, Lail K. 2010. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature, 463, 763-768[34]Worland A J. 1996. The influence of flowering time genes on environmental adaptability in European wheats. Euphytica, 89, 49-57[35]Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences of the United States of America, 103, 19581- 19586. [36]Yan L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen J L, Echenique V, Dubcovsky J. 2004. The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science, 303, 1640-1644[37]Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J. 2003. Positional cloning of the wheat vernalization gene VRN1. Proceedings of the National Academy of Sciences of the United States of America, 100, 6263-6268[38]Yang X, Westcott S, Gong X, Evans E, Zhang X Q, Lance R C M, Li C D. 2009. Amino acid substitutions of the limit dextrinase gene in barley are associated with enzyme thermostability. Molecular Breeding, 23, 61-74[39]Yant L, Mathieu J, Schmid M. 2009. Just say no: floral repressors help Arabidopsis bide the time. Current Opinion in Plant Biology, 12, 580-586[40]Ye H X, Zhang X Q, Broughton S, Westcott S, Wu D, Lance R, Li C D. 2011. A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley. Functional and Integrative Genomics, 11, 103- 110.[41]Yu J, Hu S, Wang J, Wong G K S, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X. 2002. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science, 296, 79- 92. |
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