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| Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation |
| XU Guang-yong, JIANG Jin-qi, WANG Ming, LI Jie, SU Jing-liang , REN Xiao-ming |
1 Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, P.R.China
2 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China
3 College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China |
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摘要 This study explored the effects over time of lactic acid (LA) on I Bα phosphorylation and nuclear factor-kappa B (NF- B) p65 protein expression, and on tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) mRNA levels in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) stimulated by lipopolysaccharide (LPS). I B , phosphorylated I B (p-I B ) and p65 protein levels were monitored by Western blot analysis, and TNF- and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF- and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on I B phosphorylation to prevent activation of NFB was after 6 h. These results suggest that LA reduces TNF- and IL-6 mRNA levels through decreasing I B phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF- B.
Abstract This study explored the effects over time of lactic acid (LA) on I Bα phosphorylation and nuclear factor-kappa B (NF- B) p65 protein expression, and on tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) mRNA levels in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) stimulated by lipopolysaccharide (LPS). I B , phosphorylated I B (p-I B ) and p65 protein levels were monitored by Western blot analysis, and TNF- and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF- and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on I B phosphorylation to prevent activation of NFB was after 6 h. These results suggest that LA reduces TNF- and IL-6 mRNA levels through decreasing I B phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF- B.
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Received: 09 April 2012
Accepted:
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| Fund: This study was supported by the Natural Science Foundation of Beijing, China (6072007), and the Project for the Establishment and Development of Swine Disease Clinical Diagnosis, China (KM200910020002). |
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Corresponding Authors:
Correspondence REN Xiao-ming, Tel/Fax: +86-10-80795532, E-mail: xm-ren@163.com;SU Jing-liang, Tel/Fax: +86-10-62732312, E-mail: jinglsu@yahoo.com
E-mail: xm-ren@163.com
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| About author: XU Guang-yong, Mobile: 15210922015, E-mail: 15210922015@139.com |
Cite this article:
XU Guang-yong, JIANG Jin-qi, WANG Ming, LI Jie, SU Jing-liang , REN Xiao-ming.
2013.
Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation. Journal of Integrative Agriculture, 12(6): 1073-1078.
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