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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 159-167    DOI: 10.1016/S2095-3119(20)63284-1
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In-vitro assessment for the control of Fusarium species using a lactic acid bacterium isolated from yellow pitahaya (Selenicereus megalanthus (K. Schum. Ex Vaupel Moran))
1 Universidad Nacional de Colombia, Palmira Campus, Valle 763533, Colombia
2 Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo Campus, Coahuila 25280, Mexico
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The fungistatic activity of a lactic acid bacterium, which had been isolated from yellow pitahaya cultures, against fungi associated with basal rot (Fusarium oxysporum and Fusarium fujikuroi) was measured in the present study.  Its activity was assessed in three fractions: fermented (S1), metabolic products (S2), and biomass (S3), using two fermentation substrates: Man Rogosa Sharpe agar (MRS) and potato dextrose agar (PDA).  The bacterium was molecularly identified as Lactobacillus plantarum.  S3 reduced F. fujikuroi growth by 100% over 48 h of fermentation, which occurred during the stationary phase of bacterial growth.  The three fractions’ fungistatic activity against F. fujikuroi depended on the substrate employed.  The fermentation kinetic parameters for L. plantarum indicated that its specific growth rate was 0.46 h–1, with 93.63% substrate consumption, 0.045 kg kg–1 cell yield, and 0.54 kg kg–1 product yield.  The kinetic parameters calculated will allow for bacteria production scaling.  These in-vitro trials reveal L. plantarum’s possible application as a biocontrol agent for diseases associated with Fusarium.  However, further ex-vivo and in-vivo researches are required to demonstrate its behavior in crops. 
Keywords:  lactic acid bacteria        Fusarium        fungistatic activity        biomass        yellow pitahaya  
Received: 19 January 2020   Accepted:
Fund: The authors would like to thank the Administrative Department of Science, Technology, and Innovation, Colciencias, Asoppitaya, and the Inter-American Development Bank IDB for funding.
Corresponding Authors:  Correspondence Cristobal N. AGUILAR, E-mail:   
About author:  Leidy J. VALENCIA-HERNáNDEZ, E-mail:;

Cite this article: 

Leidy J. VALENCIA-HERNÁNDEZ, Karina LÓPEZ-LÓPEZ, Eyder D. GÓMEZ-LÓPEZ, Liliana SERNA-COCK, Cristobal N. AGUILAR . 2021. In-vitro assessment for the control of Fusarium species using a lactic acid bacterium isolated from yellow pitahaya (Selenicereus megalanthus (K. Schum. Ex Vaupel Moran)). Journal of Integrative Agriculture, 20(1): 159-167.

Arasu M V, Kim D H, Kim P Il, Jung M W, Ilavenil S, Jane M, Lee K D, Al-Dhabi N A, Choi K C. 2014. In vitro antifungal, probiotic and antioxidant properties of novel Lactobacillus plantarum K46 isolated from fermented sesame leaf. Annals of Microbiology, 64, 1333–1346.
Barrios-Roblero C, Rosas-Quijano R, Salvador-Figueroa M, Gálvez-López D, Vázquez-Ovando A. 2019. Antifungal lactic acid bacteria isolated from fermented beverages with activity against Colletotrichum gloeosporioides. Food Bioscience, 29, 47–54.
Corsetti A, Settanni L. 2007. Lactobacilli in sourdough fermentation. Food Research International, 40, 539–558.
Daranas N, Roselló G, Cabrefiga J, Donati I, Francés J, Badosa E, Spinelli F, Montesinos E, Bonaterra A. 2018. Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad-spectrum activity: Biological control of bacterial plant diseases with Lactobacillus plantarum. Annals of Applied Biology, 174, 92–105.
Delavenne E, Ismail R, Pawtowski A, Mounier J, Barbier G, Le Blay G. 2012. Assessment of lactobacilli strains as yogurt bioprotective cultures. Food Control, 30, 206–213.
Dimki? I, ?ivkovi? S, Beri? T, Ivanovi? ?, Gavrilovi? V, Stankovi? S, Fira D. 2013. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. Biological Control, 65, 312–321.
Gajbhiye M H, Kapadnis B P. 2016. Antifungal-activity-producing lactic acid bacteria as biocontrol agents in plants. Biocontrol Science and Technology, 26, 1451–1470.
Hernández-Cruz A, Saldivia-Tejeda A, Silva-Rojas H V, Fuentes-Aragón D, Nava-Díaz C, Martínez-Bolaños L, Rebollar-Alviter A. 2020. Evaluation of full-season programs for the management of Fusarium wilt of blackberry caused by a new lineage of the Fusarium oxysporum species complex. Crop Protection, 134, 1–11.
Janisiewicz W J, Tworkoski T J, Sharer C. 2000. Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients. Phytopathology, 90, 1196–1200.
Konappa N M, Maria M, Uzma F, Krishnamurthy S, Nayaka S C, Niranjana S R, Chowdappa S. 2016. Lactic acid bacteria mediated induction of defense enzymes to enhance the resistance in tomato against Ralstonia solanacearum causing bacterial wilt. Scientia Horticulturae, 207, 183–192.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.
Laitila A, Alakomi H L, Raaska L, Mattila-Sandholm T, Haikara, A. 2002. Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley. Journal of Applied Microbiology, 93, 566–576.
Lan W T, Chen Y, Sheng Y, Wu H C, Yanagida F. 2012. Bio-protective potential of lactic acid bacteria isolated from fermented wax gourd. Folia Microbiologica, 57, 99–105.
León K, Mery D, Pedreschi F, León J. 2006. Color measurement in L*a*b* units from RGB digital images. Food Research International, 39, 1084–1091.
Licandro H, Ho H, Kim T, Nguyen C, Petchkongkaew A, Van Nguyen H, Chu-Ky S, Viet T, Nguyen A, Lorn D, Waché Y. 2020. How fermentation by lactic acid bacteria can address safety issues in legumes food products? Food Control, 110, 1–8.
Magnusson J, Ström K, Roos S, Sjögren J, Schnürer J. 2003. Broad and complex antifungal activity among environmental isolates of lactic acid bacteria. FEMS Microbiology Letters, 219, 129–135.
Miller G L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426–428.
Papenfort K, Bassler B L. 2016. Quorum sensing signal-response systems in Gram-negative bacteria. Natural Reviews Microbiology, 14, 576.
Salazar-González C, Serna-Cock L, Gomez-López E. 2016. Molecular characterization of Fusarium associated with basal rot of the fruit of pitahaya (Selenicereus megalanthus). Mesoamerican Agronomy, 27, 277–285. (in Spanish)
Sathe S J, Nawani N N, Dhakephalkar P K, Kapadnis B P. 2007. Antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables. Journal of Applied Microbiology, 103, 2622–2628.
Shehata M G, Badr A N, EI Sohaimy S A, Asker D, Awad T S. 2019. Characterization of antifungal metabolites produced by novel lactic acid bacterium and their potential application as food biopreservatives. Annals of Agricultural Sciences, 64, 71–78.
Soler J J, Martín-Vivaldi M, Ruiz-Rodríguez M, Valdivia E, Martín-Platero A M, Martínez-Bueno M, Peralta-Sánchez J M, Méndez M. 2008. Symbiotic association between hoopoes and antibiotic-producing bacteria that live in their uropygial gland. Functional Ecology, 22, 864–871.
Stephens K, Bentley W E, Bentley W E. 2020. Synthetic biology for manipulating quorum sensing in microbial consortia. Trends in Microbiology, 28, doi: 10.1016/j.tim.2020.03.009.
Štětina J, Chumchalova J, Halász A, Élelmiszer-Tudományi Kutatóintézet K. 2010. Production of organic acids by Lactobacillus strains in three different media. European Food Research & Technology, 230, 395–404.
Ström K, Sjögren J, Broberg A, Schnürer J. 2002. Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo (L-Phe-L-Pro) and cyclo (L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Applied and Environmental Microbiology, 68, 4322–4327.
Suskovic J, Kos B, Beganovic J, Lebos-Pavunc A, Habjanic K,  Matosic S. 2010. Antimicrobial activity - The most important property of probiotic and starter lactic acid bacteria. Food Technology Biotechnology, 48, 296–307.
Trias R, Bañeras L, Badosa E, Montesinos E. 2008. Bioprotection of Golden Delicious apples and Iceberg lettuce against foodborne bacterial pathogens by lactic acid bacteria. Journal of Food Microbiology, 123, 50–60.
Valencia-Hernández L J, López-López K, Serna-Cock L. 2016. Weissella cibaria fungistatic activity against Fusarium spp. affecting yellow pitahaya. American Journal of Applied Sciences, 13, 1354–1364.
Voulgari K, Hatzikamari M, Delepoglou A. 2010. Antifungal activity of non-starter lactic acid bacteria isolates from dairy products. Food Control, 21, 136–142.
Wang H, Yan H, Shin J, Huang L, Zhang H, Qi W. 2011. Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang Koumiss in China. Annals of Microbiology, 61, 879–885.
Xu W, Wang K, Wang H, Liu Z, Shi Y, Gao Z, Wang Z. 2020. Evaluation of the biocontrol potential of Bacillus sp. WB against Fusarium oxysporum f. sp. niveum. Biological Control, 147, 104288.
Zhang Z, Schwartz S, Wagner L, Miller W. 2000. A greedy algorithm for aligning DNA sequences. Journal of Computational Biology, 7, 203–214
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