Please wait a minute...
Journal of Integrative Agriculture  2011, Vol. 10 Issue (9): 1346-1353    DOI: 10.1016/S1671-2927(11)60127-2
GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS Advanced Online Publication | Current Issue | Archive | Adv Search |
A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers
ZHANGGuang-qing, QIJian-min, ZHANGXiao-chen, FNAGPing-ping, SUJian-guang, TAOAi-fen, LANTao, WUWei-ren, LIUAi-min
1. Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops
2. Crop Sciences Institute, Fujian Academy of Agricultural Sciences
3. College of Life Science, Fujian Agriculture and Forestry University
4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences
5. Department of Chemistry and Center for Metals in Biocatalysis, University of Minnesota
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The
objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwild
variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.
The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified
polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)
markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between
the adjacent markers.Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. Theobjective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwildvariety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplifiedpolymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM betweenthe adjacent markers.

Abstract  Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The
objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwild
variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.
The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified
polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)
markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between
the adjacent markers.Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. Theobjective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwildvariety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplifiedpolymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM betweenthe adjacent markers.
Keywords:  kenaf      genetic map      SRAP      ISSR      RAPD  
Received: 02 September 2010   Accepted:
Fund: 

This research was supported by the National NaturalScience Fundation of China (30571188 and 31000734),the National Agri-Industry Technology Research Systemfor Crops of Bast and Leaf Fiber, China (nycytx-19-E05), and the Construction of Germplasm ResourcesPlatform for Bast Fiber Crops in Fujian, China(2010N2002).

Corresponding Authors:  Correspondence QI Jian-min, Professor, Tel: +86-591-87644898, E-mail: qijm863@163.com     E-mail:  qijm863@163.com
About author:  ZHANG Guang-qing, MSc, E-mail: zgq314@163.com

Cite this article: 

ZHANGGuang-qing , QIJian-min , ZHANGXiao-chen , FNAGPing-ping , SUJian-guang , TAOAi-fen , LANTao , WUWei-ren , LIUAi-min . 2011. A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers. Journal of Integrative Agriculture, 10(9): 1346-1353.

[1]American Kenaf Society (AKS). 2000. Proceedings of the 3rdAnnual American Kenaf Society Conference. Corpus ChristiTexas, USA. pp. 23-25.

[2]Becker J, Vos P, Kuiper M, Salamini F, Heun M. 1995. Combinedmapping of AFLP and RFLP in barley. Molecular and GeneralGenetics, 249, 65-73.

[3]Chen M X, Zhang G Q, Qi J M, Zhang X C, Lin L H, Lan T, WuW R, Wu J M, Tao A F, Fang P P. 2008. Preliminary studyon construction of a genetic linkage map of kenaf using SRAPand ISSR markers. Plant Fiber Sciences in China, 30, 121-127. (in Chinese)

[4]Cheng Z, Lu B R, Baldwin B S, Sameshima K, Chen J K. 2002.Comparative studies of genetic diversity in kenaf (Hibiscuscannabinus L.) varieties based on analysis of agronomic andRAPD data. Hereditas, 136, 231-239.

[5]Coetzee R. 2004. Characterization of kenaf (Hibiscus cannabinusL.) cultivars in South Africa. MSc thesis, University of theFree State, Bloemfontein, South Africa.Dempsey J M. 1975. Fiber Crops. Rose Printing Company,Tallahassee, FL. pp. 203-233.

[6]Eujayl I, Baum M, Erskine W, Pehu E, Muehl-bauer F J. 1997.Theuse of RAPD markers for lentil genetic mapping and theevaluation of distorted F2 segregation. Euphytica, 96, 405-412.

[7]FAO. 1998. FAO Production Yearbook. vol. 32. Food andAgriculture Organization, Rome.FAO. 2003. The production and consumption of kenaf in China.ESC-Fibres Consultation no. 03/6, FAO, Rome.FAO Headquarters. 2007. Consultation on natural fibers. ESCFibresConsultation no. 07/1, FAO, Rome.Ferriol M, Picó B, Nuez F. 2003. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers.Theoretical and Applied Genetics, 107, 271-282.

[8]Japan Kenaf Association (JKA). 2000. Proceeding of theInternational Kenaf Symposium. Hiroshima, Japan. pp. 13-14.

[9]Jeuken M, van Wijk R, Peleman J, Lindhout P. 2001. An integratedinterspecific AFLP map of lettuce (Lactuca) based on two L.sativa×L. saligna F2 populations. Theoretical and AppliedGenetics, 103, 638-647.

[10]Kojima T, Nagaoka T, Noda K, Ogihara Y. 1998. Genetic linkagemap of ISSR and RAPD markers in Einkorn wheat in relationto that of RFLP markers. Theoretical and Applied Genetics,96, 37-45.

[11]Lander E S, Green P, Abrahamson J, Barlow M J, Daly M J,Lincoln S E, Newburg L. 1987. MAP-MAKER: aninteractive computer package for constructing primarygenetics linkage maps of experimental and naturalpopulations. Genomics, 1, 174-181.

[12]Li A Q. 1990. Report of the germplasm collecting for jute andkenaf in Kenya. China’s Fiber Crops, 1, 16-21. (in Chinese)

[13]Li G, Quiros C F. 2001. Sequence-related amplifiedpolymorphism (SRAP), a new marker system based on asimple PCR reaction: its application to mapping and genetagging in Brassica. Theoretical and Applied Genetics, 103,455-461.

[14]Lin Z G, Zhang X L, Nie Y C, He D H, Wu M Q. 2003.Construction of a genetic linkage map for cotton based onSRAP. Chinese Science Bulletin, 48, 2063-2067.

[15]Liu A M. 2000. World production and potential utilization ofjute, kenaf and allied fibers. In: Proceedings of the 2000International Kenaf Symposium. Hiroshima, Japan. pp. 30-35.

[16]Liu R H, Meng J L. 2003. Mapdraw: A microsoft excel macro fordrawing genetic linkage maps based on given genetic linkagedata. Hereditas, 25, 317-321.

[17]Ma H B, Qi J M, Li Y K, Liang J X, Wang T, Lan T, Chen S H,Tao A, Lin L H, Wu J M. 2008. Construction of a moleculargenetic linkage map of tobacco based on SRAP and ISSRmarkers. Acta Agronomica Sinica, 34, 1958-1963. (in Chinese)

[18]Murray M, Thompson W F. 1980. Rapid isolation of highmolecular weight plant DNA. Nucleic Acids Research, 8,4321-4325.

[19]Patterson A H, Tanksley S D, Sorrells M E. 1991. DNA markersin plant improvement. Advances in Agronomy, 46, 39-90.

[20]Sellers T, Reichert N A. 1999. Kenaf Properties, Processing andProducts. Mississippi State University, USA.Sharma R, Aggarwal R A K, Kumar R, Mohapatra T, Sharma RP. 2002. Construction of an RAPD linkage map andlocalization of QTLs for oleic acid level using recombinantinbreds in mustard (Brassica juncea). Genome, 45, 467-472.

[21]Sondur S N, Manshardt R M, Stiles J I. 1996. A genetic linkagemap of papaya based on random amplified polymorphicDNA markers. Theoretical and Applied Genetics, 93, 547-553.

[22]Staub J E, Serquen F C, Gupta M. 1996. Genetic markers, mapconstruction, and their application in plant breeding.HortScience, 31, 729-741.

[23]Tao A F, Qi J M, Li A Q, Fang P P, Lin L H, Wu J M, Wu W R.2005. The analysis of genetic diversity and relationship ofelite kenaf germplasm based on inter-simple sequence repeats.Acta Agronomica Sinica, 31, 1668-1671. (in Chinese)

[24]Venkateswarlu M, Urs R S, Nath S B, Shashidhar H E,Maheswaran M, Veeraiah T M, Sabitha M G. 2006. A firstgenetic linkage map of mulberry (Morus spp.) using RAPD,ISSR, and SSR markers and pseudotestcross mappingstrategy. Tree Genetics & Genomes, 3, 15-24.

[25]Webber C L, Bledsoe V K. 2002. Kenaf yield components andplant composition. In: Janick J, Whipkey A, eds., Trends inNew Crops and New Uses. ASHS Press, Alexandria, VA. pp.348-357.

[26]Wilson F D, Menzel M Y. 1964. Kenaf (Hibiscus cannabinus),roselle (Hibiscus sabdariffa). Economic Botany, 18, 80-91.

[27]Xu J T, Qi J M , Fang P P, Li A Q, Lin L H ,Wu J M, Tao A F.2007. Optimized CTAB protocol for extr acting genomicDNA from kenaf and improved PCR amplifications of ISSRand SRAP. Plant Fiber Sciences in China, 29, 179-183. (inChinese)

[28]Yeboah M A, Chen X H, Chen R F, Liang G H, Gu M H. 2007.A genetic linkage map of cucumber (Cucumis sativus L.)combining SRAP and ISSR markers. African Journal ofBiotechnology, 6, 2784-2791.

[29]Zhang J, Wu Y T, Guo W Z, Zhang T Z. 2000. Fast screening ofmicrosatellite markers in cotton with PAGE/silver staining.Acta Agronomica Sinica, 12, 267-269. (in Chinese)
[1] JIANG Xue-qian, ZHANG Fan, WANG Zhen, LONG Rui-cai, LI Ming-na, HE Fei, YANG Xi-jiang, YANG Chang-fu, JIANG Xu, YANG Qing-chuan, WANG Quan-zhen, KANG Jun-mei. Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.)[J]. >Journal of Integrative Agriculture, 2022, 21(3): 812-818.
[2] WANG Li-xia, WANG Jie, LUO Gao-ling, YUAN Xing-xing, GONG Dan, HU Liang-liang, WANG Su-hua, CHEN Hong-lin, CHEN Xin, CHENG Xu-zhen. Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size[J]. >Journal of Integrative Agriculture, 2021, 20(7): 1753-1761.
[3] HU Fu-chu, CHEN Zhe, WANG Xiang-he, WANG Jia-bao, FAN Hong-yan, QIN Yong-hua, ZHAO Jietang, HU Gui-bing. Construction of high-density SNP genetic maps and QTL mapping for dwarf-related traits in Litchi chinensis Sonn[J]. >Journal of Integrative Agriculture, 2021, 20(11): 2900-2913.
[4] XU Xiao-dan, FENG Jing, FAN Jie-ru, LIU Zhi-yong, LI Qiang, ZHOU Yi-lin, MA Zhan-hong. Identification of the resistance gene to powdery mildew in Chinese wheat landrace Baiyouyantiao[J]. >Journal of Integrative Agriculture, 2018, 17(01): 37-45.
[5] CHAO Wen-zheng, TANG Chuan-hong, ZHANG Jing-song, YU Ling, Honda Yoichi. Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers[J]. >Journal of Integrative Agriculture, 2018, 17(01): 130-138.
[6] SHENG Fang, CHEN Shu-ying, TIAN Jia, LI Peng, QIN Xue, WANG Lei, LUO Shu-ping, LI Jiang. Morphological and ISSR molecular markers reveal genetic diversity of wild hawthorns (Crataegus songorica K. Koch.) in Xinjiang, China[J]. >Journal of Integrative Agriculture, 2017, 16(11): 2482-2498.
[7] Ghulam Shabir, Kashif Aslam, Abdul Rehman Khan, Muhammad Shahid, Hamid Manzoor, Sibgha Noreen, Mueen Alam Khan, Muhammad Baber, Muhammad Sabar, Shahid Masood Shah, Muhammad Arif. Rice molecular markers and genetic mapping: Current status and prospects[J]. >Journal of Integrative Agriculture, 2017, 16(09): 1879-1891.
[8] WANG Hai, HE Yan, WANG Shou-cai. QTL mapping of general combining abilities of four traits in maize using a high-density genetic map[J]. >Journal of Integrative Agriculture, 2017, 16(08): 1700-1707.
[9] XIE Jing-zhong, WANG Li-li, WANG Yong, ZHANG Huai-zhi, ZHOU Sheng-hui, WU Qiu-hong, CHEN Yong-xing, WANG Zhen-zhong, WANG Guo-xin, ZHANG De-yun, ZHANG Yan, HU Tie-zhu, LIU Zhi-yong. Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics[J]. >Journal of Integrative Agriculture, 2017, 16(03): 540-550.
[10] ZHOU Yu, CHAO Gui-mei, LIU Jia-jia, ZHU Ming-qi, WANG Yang, FENG Bai-li. Genetic diversity of Ustilago hordei in Tibetan areas as revealed by RAPD and SSR[J]. >Journal of Integrative Agriculture, 2016, 15(10): 2299-2308.
[11] YU Ma, CHEN Guo-yue, ZHANG Lian-quan, LIU Ya-xi, LIU Deng-cai, WANG Ji-rui, PU Zhien, ZHANG Li, LAN Xiu-jin, WEI Yu-ming, LIU Chun-ji , ZHENG You-liang. QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii[J]. >Journal of Integrative Agriculture, 2014, 13(8): 1835-1844.
[12] HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye. Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab[J]. >Journal of Integrative Agriculture, 2014, 13(6): 1293-1302.
[13] ZHANG Kai, WU Zheng-dan, LI Yan-hua, ZHANG Han, WANG Liang-ping, ZHOU Quan-lu, TANG Dao-bin, FU Yu-fan, HE Feng-fa, JIANG Yu-chun, YANG Hang , WANG Ji-chun. ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China[J]. >Journal of Integrative Agriculture, 2014, 13(11): 2346-2361.
[14] ZHENG Fei-fei, DENG Zhi-ying, SHI Cui-lan, ZHANG Xin-ye , TIAN Ji-chun. QTL Mapping for Dough Mixing Characteristics in a Recombinant Inbred Population Derived from a Waxy×Strong Gluten Wheat (Triticum aestivum L.)[J]. >Journal of Integrative Agriculture, 2013, 12(6): 951-961.
[15] LI Feng-tao, QI Jian-min, ZHANG Gao-yang, LIN Li-hui, FANG Ping-ping, TAO Ai-fen , XU Jian-tang. Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings[J]. >Journal of Integrative Agriculture, 2013, 12(4): 610-620.
No Suggested Reading articles found!