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| Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae |
| LI Yi-ping, WUJun-xiang, LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan |
1.Key Laboratory of Applied Entomology; Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education/College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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摘要 With the long-term use of Bacillus thuringiensis (Bt) insecticide and expansion of Cry1A-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N (APN), in brush border membrane vesicles (BBMV) of Helicoverpa armigera were examined in both Cry1Ac-susceptible (96S) and Cry1Acresistant (LF120) strains, to compare Cry1Ac toxin oligomerization and pore formation in these two strains. Cry1Ac toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant H. armigera strains. The ability to form Cry1Ac oligomers and ion channels on BBMVs was also not significantly different between these two strains.
Abstract With the long-term use of Bacillus thuringiensis (Bt) insecticide and expansion of Cry1A-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N (APN), in brush border membrane vesicles (BBMV) of Helicoverpa armigera were examined in both Cry1Ac-susceptible (96S) and Cry1Acresistant (LF120) strains, to compare Cry1Ac toxin oligomerization and pore formation in these two strains. Cry1Ac toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant H. armigera strains. The ability to form Cry1Ac oligomers and ion channels on BBMVs was also not significantly different between these two strains.
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Received: 17 October 2011
Accepted:
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| Fund: This research was supported by the National Basic Research Program of China (31071693). |
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Corresponding Authors:
Correspondence GUO Yu-yuan, Tel/Fax: +86-10-62894786, E-mail: yuyuanguo@hotmail.com;LIANG Ge-mei, Tel: +86-10-62815929, E-mail: gmliang@ippcaas.cn
E-mail: yuyuanguo@hotmail.com
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| About author: LI Yi-ping, Mobile: 13759967722, E-mail: liyiping@nwsuaf.edu.cn |
Cite this article:
LI Yi-ping, WUJun-xiang , LIU Chen-xi, YUAN Xiang-qun, WU Kong-ming, LIANG Ge-mei, GUO Yu-yuan.
2012.
Comparative Analysis of Cry1Ac Toxin Oligomerization and Pore Formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae. Journal of Integrative Agriculture, 12(11): 1845-1851.
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