|
|
|
|
|
| Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L. |
| LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin |
| Key Laboratory of Plant Biotechnology in Universities of Shandong Province/College of Life Science, Qingdao Agricultural University,Qingdao 266109, P.R.China |
|
|
|
摘要 Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.
Abstract Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.
|
|
Received: 21 October 2011
Accepted:
|
| Fund: This study was supported by the National Natural Science Foundation of China (30970288 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001). |
|
Corresponding Authors:
Correspondence LIU Xin, Tel: +86-532-88030224, E-mail: liuxin6080@yahoo.com.cn
E-mail: liuxin6080@yahoo.com.cn
|
Cite this article:
LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin.
2012.
Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.. Journal of Integrative Agriculture, 12(10): 1644-1653.
|
| [1]Calvert J W, Coetzee WA, Lefer D J. 2010. Novel insightsinto hydrogen sulfide-mediated cytoprotection.Antioxidants and Redox Signaling, 12, 1203-1217.[2]Chen J,Wu FH,WangWH, Zheng C J, Lin GH, Dong X J, HeJX, Pei ZM, ZhengH L. 2011.Hydrogen sulphide enhancesphotosynthesis through promoting chloroplastbiogenesis, photosynthetic enzyme expression, and thiolredox modification in Spinacia oleracea seedlings.Journal of Experimental Botany, 62, 4481-4493.[3]Chen W, Xie X H. 2008. Research development of novelgaseous signal molecule hydrogen sulfide. MedicalRecapitulate, 14, 3704-3709. (in Chinese)[4]Desikan R, Last K, Harrett-williams R, Tagliavia C, HarterK, Hooley R, Hancock J T, Neill S J. 2006. Ethyleneinducedstomatal closure in Arabidopsis occurs viaAtrbohF-mediated hydrogen peroxide synthesis. ThePlant Journal, 47, 907-916.[5]García-Mata C, Lamattina L. 2010. Hydrogen sulph0069de, anovel gasotransmitter involved in guard cell signalling.The New Phytologist, 188, 977-984.[6]Gonugunta V K, Srivastava N, Puli M R, Roghavendra A S.2008. Nitric oxide production occurs after cytosolicalkalinization during stomatal closure induced byabscisic acid. Plant, Cell and Environment, 31, 1717-1724.[7]Hosoki R, Matsuki A N, Kimura H. 1997. The possible roleof hydrogen sulfide as an endogenous smooth musclerelaxant in synergy with nitric oxide. Biochemical andBiophysical Research Communications, 237, 527-531.[8]Kendrick M D, Chang C. 2008. Ethylene signaling: newlevels of complexity and regulation. Current Opinionin Plant Biology, 11, 479-485.[9]Kimura H. 2002. Hydrogen sulfide as a neuromodulator.Molecular Neurobiology, 26, 13-19.[10]Liu G H, Hou L X, Liu J, Liu X. 2009a. Nitric oxide induced by hydrogen peroxide mediates ethylene-inducedstomatal closure of Arabidopsis thaliana. Progress inNatural Science, 19, 8-18. (in Chinese)[11]Liu G H, Liu J, Hou L X, Tang J, Liu X. 2009b. NO mayfunction in the downstream of Ca2+ in ethylene inducedstomatal closure in Vicia faba L. Journal of MolecularCell Biology, 42, 145-155. (in Chinese)[12]Liu J, Liu G H, Hou L X, Liu X. 2010. Ethylene-inducednitric oxide production and stomatal closure inArabidopsis thaliana depending on changes incytosolic pH. Chinese Science Bulletin, 55, 2003-2009.[13]Liu X, Zhang S Q. 2000. Relationships between salicylicacid, jasmonic acid and ethylene in the regulation ofs tomatal movement s of Vic ia faba L. ActaPhytophysiologica Sinica, 26, 487-491.[14]Lowicka E, Betowski J. 2007. Hydrogen sulfide (H2S) - thethi rd gas of interes t for pharmacologis t s .Pharmacological Reports, 59, 4-24.[15]Papenbrock J, Riemenschneider A, Kamp A, Schulz-Vogt HN, Schmidt A. 2007. Characterization of cysteinedegradingand H2S-releasing enzymes of higher plantsfromthe field to the test tube and back. Plant Biology,9, 582-588.[16]Rennenberg H. 1983. Role of O-acetylserine in hydrogensulfide emission from pumpkin leaves in response tosulfate. Plant Physiology, 73, 560-565.[17]Rennenberg H. 1984. The fate excess of sulfur in higher plants.Annual Review of Plant Physiology, 35, 121-153.[18]Riemenschneider A, Nikiforova V, Hoefgen R, de Kok L J,Papenbrock J. 2005. Impact of elevated H2S onmetabolite levels, activity of enzymes and expressionof genes involved in cysteine metabolism. PlantPhysiology and Biochemistry, 43, 473-483.[19]Sekiya J, Schmid TA,Wilson LG, Filer P. 1982. Emission ofhydrogen sulfide by leaf tissue in response to Lcysteine.Plant Physiology, 70, 430-436.[20]Tang Z C. 1999. The Modern Plant Physiology ExperimentalGuide. Science Press, Beijing. pp. 152-154. (in Chinese)[21]Wang B L, Shi L, Li Y X, Zhang W H. 2010. Boron toxicityis alleviated by hydrogen sulfide in cucumber (Cucumissativus L.) seedlings. Planta, 231, 1301-1309.[22]Wang Y Q, Li L, Cui W T, Xu S, Shen W B, Wang R. 2011.Hydrogen sulfide enhances alfalfa (Medicago sativa)tolerance against salinity during seed germination bynitric oxide pathway. Plant and Soil, 351, 107-119.[23]Yong Q C, Choo C H, Tan B H, Low C M, Bian J S. 2010.Effect of hydrogen sulfide on intracellular calciumhomeostasis in neuronal cells. NeurochemistryInternational, 56, 508-515.[24]Zhang H, Hu LY, Hu K D, He YD, Wang S H, Luo J P. 2008.Hydrogen sulfide promotes wheat seed germinationand alleviates the oxidative damage against copperstress. Journal of Integrative Plant Biology, 50, 1518-1529.[25]Zhang H, Hu S L, Zhang Z J, Hu LY, Jiang C X, Wei Z J, LiuJ, Wang H L, Jiang S T. 2011. Hydrogen sulfide acts asa regulator of flower senescence in plants. PostharvestBiology and Technology, 60, 251-257.[26]Zhang H, Jiao H, Jiang C X, Wang S H, Wei Z J, Luo J P,Jones R L. 2010. Hydrogen sulfide protects soybeanseedlings against drought-induced oxidative stress.Acta Physiologiae Plantarum, 32, 849-857.[27]Zhang H, Tang J, Liu X P, Wang Y, Yu W, Peng WY, FangF, Ma D F, Wei Z J, Hu L Y. 2009. Hydrogen sulfidepromotes root organogenesis in Ipomoea batatas, Salixmatsudana and Glycine max. Journal of IntegrativePlant Biology, 51, 1086-1094. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
