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| Functional Characterization of an Aldehyde Dehydrogenase Homologue in Rice |
| YANG Sheng-hui, NIU Xiang-li, LUO Di, CHEN Chang-dong, YU Xu, TANG Wei, LU Bao-rong, LIU Yong-sheng |
1.Key Laboratory for Bio-Resource and Eco-Environment, Minstry of Education/College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China
2.College of Life Sciences, Chongqing University, Chongqing 400044, P.R.China
3.Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education/Institute of Biodiversity Science, Fudan University, Shanghai 200433, P.R.China |
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摘要 The aldehyde dehydrogenase (ALDH) superfamily of NAD(P)+-dependent enzymes, in general, oxidize a wide range of endogenous and exogenous aliphatic and aromatic aldehydes to their corresponding carboxylic acids and play an essential role in detoxification of reactive oxygen species (ROS) accumulated under the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Oryza sativa, a homologue of ALDH gene (OsALDH22) was isolated and characterized. OsALDH22 is conserved in eukaryotes, shares high homology with the orthologs from aldehyde dehydrogenase subfamily ALDH22. The OsALDH22 encodes a protein of 597 amino acids that in plants exhibit high identity with the orthologs from Zea mays, Sorghum bicolor, Hordeum vulgare and Arabidopsis thaliana, respectively, and the conserved amino acid characteristics for ALDHs are present, including the possible NAD+ binding site (F-V-G-SP- G-V-G), the catalytic site (V-T-L-E-L-G-G-K) and the Cys active site. Semi-quantitative PCR and real-time PCR analysis indicates that OsALDH22 is expressed differentially in different tissues. Various elevated levels of OsALDH22 expression have been detected when the seedlings exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Transgenic rice plants overexpressing OsALDH22 show elevated stresses tolerance. On the contrary, downregulation of OsALDH22 in the RNA interference (RNAi) repression transgenic lines manifests declined stresses tolerance.
Abstract The aldehyde dehydrogenase (ALDH) superfamily of NAD(P)+-dependent enzymes, in general, oxidize a wide range of endogenous and exogenous aliphatic and aromatic aldehydes to their corresponding carboxylic acids and play an essential role in detoxification of reactive oxygen species (ROS) accumulated under the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Oryza sativa, a homologue of ALDH gene (OsALDH22) was isolated and characterized. OsALDH22 is conserved in eukaryotes, shares high homology with the orthologs from aldehyde dehydrogenase subfamily ALDH22. The OsALDH22 encodes a protein of 597 amino acids that in plants exhibit high identity with the orthologs from Zea mays, Sorghum bicolor, Hordeum vulgare and Arabidopsis thaliana, respectively, and the conserved amino acid characteristics for ALDHs are present, including the possible NAD+ binding site (F-V-G-SP- G-V-G), the catalytic site (V-T-L-E-L-G-G-K) and the Cys active site. Semi-quantitative PCR and real-time PCR analysis indicates that OsALDH22 is expressed differentially in different tissues. Various elevated levels of OsALDH22 expression have been detected when the seedlings exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Transgenic rice plants overexpressing OsALDH22 show elevated stresses tolerance. On the contrary, downregulation of OsALDH22 in the RNA interference (RNAi) repression transgenic lines manifests declined stresses tolerance.
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Received: 28 April 2011
Accepted:
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| Fund: This work was supported by the National 973 Program of China (2011CB100401), the Chongqing Municipal Science and Technology Research Project of China (2010AA1019), the National Science and Technology Key Projects of China (2009ZX08001-011B and 2009ZX08009-072B) and the National Science Fund for Distinguished Young Scholars of China (30825030). |
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Corresponding Authors:
Correspondence LIU Yong-sheng, Tel/Fax: +86-28-85460570, E-mail:liuyongsheng1122@yahoo.com.cn
E-mail: liuyongsheng1122@yahoo.com.cn
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| About author: YANG Sheng-hui, Mobile: 18280872768, E-mail: yangshenghui_1013@163.com |
Cite this article:
YANG Sheng-hui, NIU Xiang-li, LUO Di, CHEN Chang-dong, YU Xu, TANG Wei, LU Bao-rong, LIU Yong-sheng.
2012.
Functional Characterization of an Aldehyde Dehydrogenase Homologue in Rice. Journal of Integrative Agriculture, 12(9): 1434-1444.
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| [1]Akira Y, Andrey R, Lily C H, Cheng C. 1998. Human aldehyde dehydrogenase gene family. European Journal of Biochemistry, 251, 549-557. [2]Ashraf M. 2010. Inducing drought tolerance in plants: Recent advances. Biotechnology Advances, 28, 169-183. [3]Barclay K D, Mckersie B D. 1994. Peroxidation reactions in plant membranes-effects of free fatty acids. Lipids, 29, 877-882. [4]Bartels D. 2001. Targeting detoxification pathways: an efficient approach to obtain plants with multiple stress tolerance? Trends in Plant Science, 6, 284-286. [5]Bouché N, Fait A, Bouchez D, Møller S G, Fromm H. 2003. Mitochondrial succinic-semialdehyde dehydrogenase of the gamma-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. Proceedings of the National Academy of Sciences of the United States of America, 100, 6843-6849. [6]Bustin S A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology, 25, 169-193. [7]Chen T H, Murata N. 2002. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology, 5, 250-257. [8]Choi H, Hong J, Ha J, Kang J, Kim S Y. 2000. ABFs, a family © 2012, CAAS. All rights reserved. Published by Elsevier Ltd. of ABA-responsive element binding factors. Journal of Biological Chemistry, 275, 1723-1730. [9]Claire G, Annaick M, Benedicte C. 2004. Real-time PCR: what relevance to plant studies? Journal of Experimental Botany, 55, 1445-1454. [10]Deuschle K, Funck D, Hellmann H, Daschner K, Binder S, Frommer W B. 2001. A nuclear gene encoding mitochondrial deltapyrroline-5-carboxylate dehydrogenase and its potential role in protection from proline toxicity. The Plant Journal, 27, 345-356. [11]Gao C X, Han B. 2009. Evolutionary and expression study of the aldehyde dehydrogenase (ALDH) gene superfamily in rice (Oryza sativa). Gene, 431, 86-94. [12]Gao Z, Loescher W H. 2000. NADPH supply and mannitol biosynthesis. Characterization, cloning, and regulation of the nonreversible glyceraldehydes-3-phosphate dehydrogenase in celery leaves. Plant Physiology, 124, 321-330. [13]Guo H S, Fei J F, Xie Q, Chua N H. 2003. A chemical-regulated inducible RNAi system in plants. The Plant Journal, 34, 383-392. [14]Hayashi H, Alia I, Mustardy L, Deshnium P, Miki I I, Murata N. 1997. Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress. The Plant Journal, 12, 133-142. [15]Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6, 271-282. [16]Higo K, Ugawa Y, Iwamoto M, Korenaga T. 1999. Plant cisacting regulatory DNA elements (PLACE) database. Nucleic Acids Research, 27, 297-300. [17]Huang W Z, Ma X R, Wang Q L, Gao Y F, Xue Y, Niu X L, Yu G R, Liu Y S. 2008. Significant improvement of stress tolerance in tobacco plants by overexpressing a stressresponsive aldehyde dehydrogenase gene from maize (Zea mays). Plant Molecular Biology, 68, 451-463. [18]Ishitani M, Nakamura T, Han S Y, Takabe T. 1995. Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. Plant Molecular Biology, 27, 307-315. [19]Jimenez-Lopez J C, Gachomo E W, Seufferheld M J, Simeon O, Kotchoni S O. 2010. The maize ALDH protein superfamily: linking structural features to functional specificities. BMC Structural Biology, 10, 43. Kaplan B, Davydov O, Knight H, Galon Y, Knight M R, Fluhr R, Frommc H. 2006. Rapid transcriptome changes induced by cytosolic Ca2+ transients reveal ABRErelated sequences as Ca2+-responsive cis elements in Arabidopsis. The Plant Cell, 18, 2733-2748. [20]Kenneth J L, Thomas D S. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. METHODS, 25, 402-408. [21]Kirch H H, Bartels D, Wei Y, Schnable P S, Wood A J. 2004. The ALDH gene superfamily of Arabidopsis. Trends in Plant Science, 9, 371-377. [22]Kirch H H, Schlingensiepen S, Kotchoni O S, Sunkar R, Bartels D. 2005. Detailed expression analysis of selected genes of the aldehyde dehydrogenase (ALDH) gene superfamily in Arabidopsis thaliana. Plant Molecular Biology, 57, 315-332. [23]Kishor P B K, Hong Z, Miao G H, Hu C A A, Verma D P S. 1995. Over-expression of delta-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiology, 108, 1387-1394. [24]Li Y, Nakazono M, Tsutsumi N, Hirai A. 2000. Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice. Gene, 249, 67-74. [25]Lindahl R. 1992. Aldehyde dehydrogenases and their role in carcinogenesis. Critical Reviews in Biochemistry and Molecular Biology, 27, 283-335. [26]Liu Z H, Zhang H M, Li G L, Guo X L, Chen S Y, Liu G B. Zhang M Y. 2011. Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase. Euphytica, 178, 363-372. [27]Mao J, Zhang Y C, Sang Y, Li Q H, Yang H Q. 2005. A role for Arabidopsis cryptochromes and COP1 in the regulation of stomatal opening. Proceedings of the National Academy of Sciences of the United States of America, 102, 12270-12275. [28]Marcotte Jr W R, Russell S H, Quatrano R S. 1989. Abscisic acidresponsive sequences from the Em gene of wheat. The Plant Cell, 1, 969-976. [29]McCue K F, Hanson A D. 1992. Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression. Plant Molecular Biology, 18, 1-11. [30]Missihoun T D, Schmitz J, Klug R, Kirch H H, Bartels D. 2011. Betaine aldehyde dehydrogenase genes from Arabidopsis with different sub-cellular localization affect stress responses. Planta, 233, 369-382. [31]Nakamura T, Yokota S, Muramoto Y, Tsutsui K, Oguri Y, Fukui K. 1997. Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes. The Plant Journal, 11, 1115-1120. [32]Nakazono M, Tsuji H, Li Y H, Saisho D, Arimura S, Tsutsumi N, Hirai A. 2000. Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions. Plant Physiology, 124, 587-598. [33]Perozich J, Nicholas H, Wang B C, Lindahl R, Hempel J. 1999. Relationships within the aldehyde dehydrogenase extended family. Protein Science, 8, 137-146. [34]Pooja B M, Vadez V, Sharma K K. 2008. Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Reports, 27, 411-424. [35]Porebskis, Baile Y G, Baumb B R. 1997. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphend components. Plant Molecular Biology Reporter, 15, 8-15. [36]Simone M R, Maxuel O A, Ana P S G, Fabio M D, Maria C B P, Elizabeth P B F. 2006. Arabidopsis and tobacco plants ectopically expressing the soybean antiquitin-like ALDH7 gene display enhanced tolerance to drought, salinity, and oxidative stress. Journal of Exprimental Batany, 57, 1909-1918. [37]Simpson S D, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2003. Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. The Plant Journal, 33, 259-270. [38]Sophos N A, Pappa A, Ziegler T L, Vasiliou V. 2001. Aldehyde dehydrogenase gene superfamily: the 2000 update. Chemico Biological Interactions, 130, 323-337. [39]Sunkar R, Bartels D, Kirch H H. 2003. Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. The Plant Journal, 35, 452-464. [40]Tsuji H, Meguro N, Suzuki Y, Tsutsumi N, Hirai A, Nakazono M. 2003. Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re-aeration in rice. FEBS Letters, 546, 369-373. [41]Vasilis V, Aglaia P, Dennis R P. 2000. Role of aldehyde dehydrogenases in endogenous and xenobiotic metabolism. Chemico-Biological Interactions, 129, 1-19. [42]Vasiliou V, Bairoch A, Tipton K F, Nebert D W. 1999. Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping. Pharmacogenetics, 9, 421-434. [43]Wei Y L, Lin M, Oliver D J, Schnable P S. 2009. The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis. BMC Biochemistry, 10, 7. Weretilnyk E A, Hanson A D. 1990. Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in aptation to salinity and drought. Proceedings of the National Academy of Sciences of the United States of America, 87, 2745-2749. [44]Wood A J, Saneoka H, Rhodes D, Joly R J, Goldsbrough P B. 1996. Betaine aldehyde dehydrogenase in sorghum. Plant Physiology, 110, 1301-1308. [45]Yoshida A. 1991. Genetic polymorphisms of alcohol metabolizing enzymes related to alcohol sensitivity and alcoholic diseases. Alcohol and Alcoholism, 29, 693-696. [46]Yoshida A, Rzhetsky A, Hsu L C, Chang C. 1998. Human aldehyde dehydrogenase gene family. European Journal of Biochemistry, 251, 549-557. [47]Zhang N, Si H J, Wen G, Du H H, Liu B L, Wang D. 2011. Enhanced drought and salinity tolerance in transgenic potato plants with a BADH gene from spinach. Plant Biotechnology Reports, 5, 71-77. [48]Zhou S F, Chen X Y, Zhang X G, Li Y X. 2008. Improved salt tolerance in tobacco plants by co-transformation of a betaine synthesis gene BADH and a vacuolar Na+/H+ antiporter gene SeNHX1. Biotechnology Letters, 30, 369-376. |
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